Method for wall breaking of oil-containing microalgae and assistant assisted extraction of oil

An auxiliary extraction and microalgae technology, which is applied in the direction of fat oil/fat production and fat production, can solve the problems of destroying the original liquid components in cells, large amount of organic solvents, and low oil extraction rate, so as to improve the effect of wall breaking and oil extraction The effect of high rate and reduced dosage

Active Publication Date: 2015-12-23
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the low oil extraction rate in existing oil-containing microalgae wall breaking and oil extraction, easy to destroy the original liquid components in cells, harsh reaction conditions, large amount of organic solvents, serious environmental pollution, high energy consumption or narrow application range, etc. Disadvantages, provide a new method for breaking the wall of oil-containing microalgae and extracting its oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and aiding the extraction of oil with additives of the present invention.

[0057] (1) Put 5 g of Nannochloropsis dry powder into a conical flask, add water to prepare a 25% by weight suspension, adjust the pH value to 8.5, and stir at 80° C. for 4 hours;

[0058] (2) Regulate the pH value to 5. In terms of Nannochloropsis dry powder, cellulase (Beijing Hongrun Baoshun Technology Co., Ltd., 2000IU / g, the same below), pectinase ( Beijing Hongrun Baoshun Technology Co., Ltd., 20000IU / g, the same below), acid protease (Beijing Hongrun Baoshun Technology Co., Ltd., 50000IU / g, the same below) and lysozyme (Sinopharm Chemical Reagent Co., Ltd., 20000IU / g , the same below), reacted at 50°C for 5h;

[0059] (3) Add 2mL DMSO, stir at 25°C for 45min;

[0060] (4) Add 10mL ethanol, 20ml n-hexane, stir for 30min, centrifuge at 5000rpm for 10min, take the organic solvent layer containing oi...

Embodiment 2

[0062] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and aiding the extraction of oil with additives of the present invention.

[0063] (1) Put 5g of chlorella dry powder into a conical flask, add water to prepare a 15% by weight suspension, adjust the pH value to 10, and stir at 60°C for 6h;

[0064] (2) Adjust the pH value to 4, add cellulase, pectinase, acid protease and lysozyme in an amount of 200 IU / g, and react at 40°C for 6 hours;

[0065] (3) Add 1mL DMSO and stir at 20°C for 30min;

[0066] (4) Add 5mL of methanol and 10mL of chloroform, stir for 40min, centrifuge at 4000rpm for 15min, take the organic solvent layer containing oil, then distill under reduced pressure at 100-200Pa at 60°C, and dry at constant weight to obtain oil, oil The extraction rate is shown in Table 1.

Embodiment 3

[0068] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and aiding the extraction of oil with additives of the present invention.

[0069] (1) Put 5 g of Scenedesmus dry powder into a conical flask, add water to prepare a 30% by weight suspension, adjust the pH to 12, and stir at 100°C for 2 hours;

[0070] (2) Adjust the pH value to 5.5, add cellulase, pectinase, acid protease and lysozyme in an amount of 300 IU / g based on Scenedesmus dry powder, and react at 60°C for 4 hours;

[0071] (3) Add 3 mL of DMSO and stir at 30°C for 60 min;

[0072] (4) Add 15mL of ethanol, 30mL of petroleum ether (Beijing Chemical Plant, analytically pure), stir for 50min, centrifuge at 6000rpm for 5min, take the organic solvent layer containing grease, and then distill under reduced pressure at 100-200Pa at 50°C for constant Heavy drying, to obtain oil, the extraction rate of oil is shown in Table 1.

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Abstract

The invention discloses a method for wall breaking of oil-containing microalgae and assistant assisted extraction of oil. The method comprises the following steps: 1, adjusting the pH value of a suspension of the oil-containing microalgae to 7.5-14, and carrying out hydrothermal treatment; 2, carrying out an enzymatic hydrolysis reaction in the presence of an enzyme; 3, adding an assistant for promoting precipitation of oil in the oil-containing microalgae, and reacting; and 4, adding an organic solvent to extract, and separating the oil. The method has the advantages of high oil extraction rate, mild operation conditions, effective maintenance of nutrition components of the microalgae, small dosage of the organic solvent, environmental protection and low energy consumption, is suitable for Nannochloropsis oceanica, chlorella, diatom, scenedesmus, Monoraphidium sp. and other microalgae, and has a wide application range.

Description

technical field [0001] The invention relates to the technical field of microalgae bioengineering, in particular to a method for breaking the wall of oily microalgae and extracting oil with the aid of additives. Background technique [0002] Microalgae refer to tiny algae groups whose shape can only be distinguished under a microscope, and are the most important primary productivity in the biosphere. Algae has the advantages of large biomass, short production cycle, various types, high oil content, and strong environmental adaptability. It is a good raw material for biodiesel. In addition, microalgae also contains a variety of vitamins, carotene, protein, fatty acids and other components, which are the source of medicinal active substances. [0003] However, microalgae oils are wrapped in cells, and most of the bioactive substances of microalgae such as proteins, carotenoids, and microalgae polysaccharides are present in biological cells. Because microalgae cells are small ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 吴冲冲荣峻峰林伟国宗明生
Owner CHINA PETROLEUM & CHEM CORP
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