A method for producing microalgae oil by using flue gas

A technology for microalgae oil and flue gas, applied in the fields of biotechnology and bioenergy, can solve problems such as low efficiency, and achieve the effects of increasing growth rate, improving fixation efficiency, and high carbon fixation efficiency

Active Publication Date: 2019-06-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For existing algal species that cannot tolerate and absorb high concentrations of CO 2 , fixed CO 2 The problem of low efficiency, the present invention provides a method of using flue gas to produce microalgae oil

Method used

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  • A method for producing microalgae oil by using flue gas
  • A method for producing microalgae oil by using flue gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) In a 10L photobioreactor, add Scenedesmus obliquus FSH-Y2 seed solution and microalgae culture medium prepared in Example 1, the amount of FSH-Y2 seed solution added is 400mL, the pH value of the microalgae culture medium Adjusted to 10, the addition amount is 8L, the cultivation light intensity is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced 2 The content is 3v%, NO and NO 2 Content is 50×10 -6 (v / v), SO 2 Content is 60×10 -6 (v / v).

[0028] (2) After cultivating for 4 days, add the seed liquid of Monopodella SS-B1 and seed liquid of Cellulosa SS-B7 prepared in Example 1, and the addition amount of the two algae seed liquids is 200mL, and adjust the pH of the microalgae culture system 8, continuous light culture, the light intensity is 5000Lux; CO2 in the flue gas 2 The content is 30v%, NO and NO 2 The content is 500×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0029] (3) After 7 days...

Embodiment 2

[0031] (1) In a 10L photobioreactor, add Scenedesmus obliquus FSH-Y2 seed solution and microalgae medium prepared in Example 1, the amount of FSH-Y2 seed solution added is 800mL, the pH value of the microalgae medium Adjusted to 12, the addition amount is 8L, the light intensity of cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v), SO 2 The content is 100×10 -6 (v / v).

[0032] (2) After culturing for 2 days, insert the seed liquid of Monopodella SS-B1 and seed liquid of Cellulosa SS-B7 prepared in Example 1, and the addition amount of the two algae seed liquids is 200mL, and adjust the pH of the microalgae culture system 9, continuous light culture, the light intensity is 5000Lux; CO2 in the flue gas 2 The content is 30v%, NO and NO 2 The content is 500×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0033] (3) After culturing...

Embodiment 3

[0035] (1) In a 10L photobioreactor, add Scenedesmus obliquus FSH-Y2 seed solution and microalgae medium prepared in Example 1, the amount of FSH-Y2 seed solution added is 600mL, and the pH value of the microalgae medium Adjusted to 11, the addition amount is 8L, the light intensity of cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced 2 The content is 4v%, NO and NO 2 Content is 50×10 -6 (v / v), SO 2 Content is 60×10 -6 (v / v).

[0036] (2) After cultivating for 3 days, add the seed liquid of Monopodella SS-B1 and seed liquid of Cellulosa SS-B7 prepared in Example 1. The amount of the seed liquid of the two algae is 300mL, and adjust the microalgae culture system. The pH is 9, continuous light culture, the light intensity is 5000Lux; 2 The content is 40v%, NO and NO 2 The content is 500×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0037] (3) After culturing for 8 days, enter the stable gr...

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Abstract

The invention discloses a method for producing microalgal oil by using flue gas, wherein the method includes the steps: (1) adding a microalgae culture medium and a scenedesmus obliquus FSH-Y2 seed liquid into a photobioreactor, regulating the pH of the culture system to 10-12, introducing flue gas with the CO2 volume content of 1 v%-5 v%, and culturing for 2-5 days; and (2) regulating the pH value of the culture system to 8-10, inoculating with monoraphidium sp. SS-B1 and ankistrodesmus sp. SS-B7 seed liquids, carrying out mixed culture, introducing flue gas with the CO2 volume content of 5 v%-45 v%, culturing to a stable stage under continuous illumination, and harvesting microalgae cells, wherein the scenedesmus obliquus FSH-Y2, the monoraphidium sp. SS-B1 and the ankistrodesmus sp. SS-B7 are respectively preserved in China General Microbiological Culture Collection Center on September 11, 2012 and on April 15, 2013 respectively and have the preservation numbers of CGMCC No.6551, CGMCC No.7479 and CGMCC No.7478 respectively. The method improves the tolerance and the solubility of the microalgae culture system on high-concentration CO2, improves the carbon sequestration efficiency, obviously increases the yield of the microalgal oil, and can realize purification of the flue gas.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for producing microalgae oil by using flue gas. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12P39/00C12P7/64C12R1/89
Inventor 师文静廖莎高大成孙启梅李晓姝姚新武樊亚超王领民
Owner CHINA PETROLEUM & CHEM CORP
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