Highly oil-containing monoraphidium and culture and application thereof

A single needle algae and oil technology, which is applied in the fields of biotechnology and bioenergy, can solve the problems of not involving oil content, affecting the growth of algae cells, and bacterial contamination, so as to avoid photosynthesis inhibition, high total lipid content, and high growth rate. fast effect

Active Publication Date: 2015-05-13
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

CN102703326A discloses a microalgae with high CO2 tolerance and fixation rate and its breeding method, but the algae strain provided by the patent does not involve the oil content of the strain
[0005]Liu Pinghuai et al. (Effects of organic carbon sources on cell growth, lipid accumulation and photosynthesis of Monospora, Bioengineering, 2012, 33(18): 224-246) Introduced a production method of using organic carbon sources to cultivate Monostipella. Although the biomass exceeded 10g / L at the end of the cultivation, this method is a heterotrophic culture method of Monostipene. During the cultivation process, organic carbon sources such as glucose were used to To achieve cell growth, this culture method does not use inorganic carbon sources such as CO2 to be economical, and the addition of organic carbon sources is prone to bacterial contamination during the culture process, which affects the growth of algae cells

Method used

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  • Highly oil-containing monoraphidium and culture and application thereof
  • Highly oil-containing monoraphidium and culture and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1 Separation and acclimatization screening to obtain Monostipella SS-B1

[0026] (1) Obtaining the starting algae strain: In October 2011, 50 mL of water samples were retrieved from Longhu Lake in Shoushan Leisure Resort, Dubolte Mongolian Autonomous County, Heilongjiang, and inoculated into 200 mL of BG11 medium for enrichment culture. The light intensity for cultivation is 5000Lux, the temperature is 25°C, the light-dark cycle is 24h, and the light-dark time ratio is 14:10. After about half a month of cultivation, the culture medium turns green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000Lux and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cultivation, the cultivation temperature is 25°C, and the light...

Embodiment 2

[0031] Example 2 Identification of algae strains

[0032] The DNA of the obtained SS-B1 algae cells was extracted using the CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Monoraphidium sp. It has the greatest similarity, its BLASTn value is 2586, and its Max index value is 99%, so it can be determined that SS-B1 is Monospirulina ( Monoraphidium sp. ).

[0033] Single needle algae SS-B1 is a kind of freshwater green algae, the algae cells are long leaf-shaped, green, the length of the algae is 10-20 μm, the width is 2-4 μm, and it contains pigment. The flat algae colonies are S-shaped and dark green.

Embodiment 3

[0034] Example 3 Application of Monostipella SS-B1

[0035] The algae liquid of SS-B1 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formula of BG11 medium is shown in Table 2 and Table 3. The cultivation was carried out in a laboratory self-made column reactor (with an inner diameter of 40mm and a height of 500mm), the density OD after inoculation 690 is 0.2. CO was introduced from the bottom of the reactor 2 Flue gas with a content of 30v%, of which SO 2 The content is 400×10 -6 (v / v). During the cultivation process, the light intensity was 5000Lux, the cultivation temperature was 28°C, the pH value was controlled between 7-9, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 7 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, and the dry weight was measured after freeze-drying, the biomass production was calculated, and the total lipid co...

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Abstract

The invention provides a highly oil-containing monoraphidium and culture and application thereof, the monoraphidium strain is SS-B1, the classification and nomenclature of the monoraphidium is monoraphidium sp, the monoraphidium is preserved in CGMCC (China General Microbiological Culture Collection Center) on April 15, 2013, and the preservation number is CGMCC No.7479. The monoraphidium strain is able to tolerate high concentrations of CO2 and SO2, highly oil-containing biological matters can be obtained by light autotrophic growth by use of CO2 and SO2 containing waste gas or flue gas, the carbon fixation efficiency is high, cell total lipid content accounts for more than 40% of dry cell weight, and biodiesel can be produced.

Description

technical field [0001] The invention belongs to the field of biotechnology and bioenergy, in particular to a CO tolerant 2 and SO 2 Lipid-rich monospina and its cultural application. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64B01D53/84B01D53/62C12R1/89
CPCB01D53/62B01D53/84B01D2257/504C12P7/64C12N1/125C12R2001/89Y02E50/10Y02P20/59Y02A50/20
Inventor 师文静高大成廖莎金平王领民李晓姝张霖王崇辉姚新武
Owner CHINA PETROLEUM & CHEM CORP
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