Method for producing lipid through mixed culture of microalgae

A mixed culture and microalgae technology, which is applied in the field of biotechnology and bioenergy, can solve the problems of unfavorable algae absorption and utilization, low solubility, and reduced carbon sequestration efficiency, so as to alleviate the greenhouse effect and exhaust pollution problems, and the multi-biomass and oil Content, the effect of improving the fixation efficiency

Active Publication Date: 2017-12-12
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
[0005] The above patents either cannot efficiently utilize CO 2 Production of oils or fats, or obtaining biomass with insufficient oil content
Especially in practical applications, when the environment CO 2 When the volume fraction is greater than 5v%, the growth of most microalgae will be inhibited, and the carbon fixation efficiency will be low
At the same time, general microalgae are suitable for growth under neutral conditions, and it is not conducive to the growth of microalgae under acidic or alkaline conditions, and microalgae use CO 2 Usually HCO dissolved in water 3 - It exists in the form of ions, and the solubility of carbon dioxide is low in a neutral environment, which is not conducive to the absorption and utilization of algae
Moreover, high concentrations of SOx, NOx and other gases in the fossil fuel exhaust gas will also inhibit the growth of microalgae and reduce the efficiency of carbon fixation

Method used

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  • Method for producing lipid through mixed culture of microalgae
  • Method for producing lipid through mixed culture of microalgae
  • Method for producing lipid through mixed culture of microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Preparation of Microalgae Seed Solution

[0024] BG11 medium was used for microalgae culture, and the medium formulations are shown in Table 1 and Table 2.

[0025] Table 1 BG11 medium

[0026]

[0027] *Table 2 Composition of A5+Co solution in Table 1

[0028]

[0029] First, prepare BG11 liquid medium according to Table 1 and Table 2, adjust the pH of the medium for cultivating Pseudochlorella keseizi FSH-Y3 and Scenedesmus obliquus FSH-Y2 to 10, and culture Scenedesmus MH-04 and single The pH of the culture medium of Spirulina SS-B1 was adjusted to 8.0, and then inoculated in the above medium respectively, and cultured in a constant temperature light shaker, the culture temperature was 25°C, the light cycle was 24h, and the light-dark time ratio was 14:10 , the light intensity is 5000Lux, 120rpm shaking culture to the logarithmic growth phase, to obtain Pseudochlorella kesperi FSH-Y3 seed liquid, Scenedesmus obliquus FSH-Y2 seed liquid, Scenedesmus MH...

Embodiment 2

[0030] Example 2 Preparation of microalgae oil

[0031] (1) In the photobioreactor, add Pseudochlorella kescheria FSH-Y3 seed solution and microalgae medium prepared in Example 1, the volume ratio of seed solution to medium is 1:10, and the medium adopts BG11 Culture medium, the pH value of the medium is controlled at 10, the light intensity of the culture is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and a mixed gas of nitrogen and carbon dioxide is introduced, and the carbon dioxide content is 5v%.

[0032] (2) After 2 days of cultivation, insert the Scenedesmus MH-04 seed solution prepared in Example 1, the volume ratio of the seed solution to the medium is 1:10, the pH of the medium is controlled at 8, and a mixture of nitrogen and carbon dioxide is introduced gas, of which CO 2 The content is 30v%.

[0033] (3) After cultivating for 7 days, it enters the stable period, ends the culturing, harvests microalgae cells by centrifugation, and vacuum ...

Embodiment 3

[0034] Example 3 Preparation of microalgae oil

[0035] (1) In the photobioreactor, add Pseudochlorella kescheria FSH-Y3 seed solution and microalgae medium prepared in Example 1, the volume ratio of seed solution to medium is 1:10, and the medium adopts BG11 Culture medium, the PH value of the medium is controlled at 12, the light intensity of the culture is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the mixed gas of nitrogen and carbon dioxide is introduced, and the carbon dioxide content is 3v%.

[0036] (2) After culturing for 3 days, insert the seed solution of Monostipella SS-B1 prepared in Example 1, the volume ratio of the seed solution to the medium is 1:10, the pH of the medium is controlled at 10, and nitrogen and carbon dioxide are introduced into the solution. Mixed gas, where CO 2 The content is 35v%.

[0037] (3) After cultivating for 8 days, it enters the stationary phase, ends the culturing, harvests the microalgae cells by cent...

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Abstract

The invention discloses a method for producing lipid through mixed culture of microalgae. The method comprises the following steps: (1) adding a microalgae medium and a parachlorella kessleri FSH-Y3 seed solution to a photobioreactor, regulating pH value of a culture system to 10-12, supplying gas that a CO2 content is 1-5v%, and conducting culture for 2-5 days; (2) regulating the pH value of the culture system to 7-10, inoculating the culture system with desmodesmus sp. MH-04 and/or monoraphidium sp. SS-B1 seed solutions/seed solution and implementing mixed culture, and supplying gas that a CO2 content is 5-45v%; and (3) conducting the culture until the end of a stationary growth phase, and collecting microalgae cells. According to the method provided by the invention, the tolerance and the solubility of the microalgae culture system to high-concentration CO2 are improved, a carbon fixation efficiency is improved and the yield of the microalgae lipid is obviously improved; and the method is applicable to the production of biodiesel.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for producing oil by mixed culture of microalgae. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64B01D53/84C12R1/89
CPCB01D53/84B01D2257/302B01D2257/504C12P7/6463Y02A50/20Y02E50/10Y02P20/59
Inventor 师文静孙启梅廖莎王鹏翔李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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