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Obtaining and application of oil-producing monoraphidium LB59

A technology of monopods and algae strains, which is applied in the field of screening and cultivation of oleaginous microalgae to achieve the effect of preventing damage and reducing costs

Inactive Publication Date: 2015-01-07
INST OF AQUATIC LIFE ACAD SINICA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of insufficient fine algae strains used to produce microalgae oil in the prior art, and to provide a kind of Obtaining and its application, that is, collecting water samples in the wild waters of Inner Mongolia, separating and purifying a kind of oil-producing microalgae with stress resistance and easy open culture, and establishing methods for cultivating and collecting

Method used

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  • Obtaining and application of oil-producing monoraphidium LB59
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  • Obtaining and application of oil-producing monoraphidium LB59

Examples

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example 1

[0040] Example 1. Semi-continuous cultivation in a circular photoreactor

[0041] Prepare the culture medium according to the medium formula described above, at 0.1m 2 Inoculate and cultivate in a circular photoreactor, and the volume of the culture medium is 20L. Initial OD 680 The value is 0.05, the temperature is controlled at 30-35°C, and the light intensity is 200μE / m 2 s continuous light, pass CO2 into the culture medium for 1 hour every day 2 , and sample OD every day 680 value, OD 680 When the value reaches 6.0, collect 1 / 2 of the algae liquid and supplement 1 / 2 of fresh medium for semi-continuous culture, and then collect the algae liquid every 5 days to feed the same volume of medium. Adjust the pH to 6 with dilute hydrochloric acid in the collection container, add chitosan to 5.0 mg / L, and the flocculation and sedimentation efficiency reaches over 90% in 30 minutes; remove the supernatant from the flocculated algae liquid to obtain concentrated algae Cells, fr...

example 2

[0044] Example 2. Outdoor track pool cultivation

[0045] According to the medium formula of the algae strain, the local groundwater in Inner Mongolia was used to prepare the culture medium, and the outdoor area of ​​Ordos, Inner Mongolia was 5m 2 Runway pools for open culture (see Figure 4), the volume of culture medium is 750L. The flow rate of the runway pool is controlled by a wheel-shaped agitator to 0.04m / s; the water temperature is about 30°C during the day and about 24°C at night; the light intensity varies with the weather, and the average light intensity during the day is 600μE / m 2 s, in a dark state at night; sample and measure OD every day 680 value, when cultured to OD 680 When the value reaches 2.0, start to collect 1 / 5 of the volume of algae cell liquid, then add 1 / 5 of the fresh culture liquid for semi-continuous culture, and then collect feed once every 3 days. The collected algae liquid is observed under a microscope and maintains more than 99% pure spec...

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Abstract

The present invention disclosed the obtaining and application of a single -needle algae LB59, which involves the screening and cultivation technology of oil -producing microalgae; the algae species obtained from the separation of the field environment are analyzed and identified by 18srdna sequences, and the algae is determined that the algae is a single needle.Members of Monoraphidium, the sequence is seqno.1.Monopolysis LB59 can tolerate high -concentration of sodium bicarbonate, easy to carry out open culture, rich in oil. In the case of maintaining the growth and collection rate of 23.6g / m2D%, Fatty acids contain more than 20%18 carbon 3 ole acid and 18 carbon 4 ole acid.

Description

technical field [0001] The invention relates to the screening and cultivation technology of oleaginous microalgae, and in particular to the acquisition and application of a single needle algae oleaginous LB59. Specifically, the present invention collects water samples from the field, and obtains an oleaginous algal strain that can be used as a raw material for oil production through separation and purification. Background technique [0002] There are more than 40,000 species of algae identified on Earth, some of which are rich in oil. The oil extracted from oil algae can be converted into biodiesel through reaction with methanol, which is considered the most likely to replace the depleted petroleum source of diesel. The 16-carbon and 18-carbon fatty acids it contains are most beneficial for biodiesel production. Algal oils also contain unsaturated fatty acids that can be added to animal feed to promote growth. Screening and obtaining algal species that are easy to cultiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C11B1/10A23K1/16C12R1/89
Inventor 孔任秋徐旭东席玮芳高宏方仙桃
Owner INST OF AQUATIC LIFE ACAD SINICA
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