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A kind of cultivation method of single needle algae

A method for culturing and a technique for monocystis algae, applied in the field of cultivating monocythium algae, can solve the problems of high oil content and low cultivation cost of monocythium algae, and achieve the effects of high oil content, low cultivation cost and easy quality control.

Active Publication Date: 2020-03-24
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned defects in the prior art, and to provide a method for cultivating Monospina, the method can realize the continuous cultivation of Monospina, the operation of the cultivation process is stable, the quality control is easy, and the oil content of Monospina is low. High, and all the culture fluid can be recycled during the cultivation process, the cultivation cost is low and there will be no problems of waste liquid discharge and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] This example is used to illustrate the method of cultivating Monospora using a closed photobioreactor.

[0060] In this example, the cultivation of Monostipella biomass growth stage and the cultivation of oil accumulation stage were all carried out in a closed photobioreactor. Among them, there is one closed photobioreactor for cultivation in the biomass growth stage, one closed photobioreactor for cultivation in the oil accumulation stage, and one separation and storage device.

[0061] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium. The content of sodium nitrate in each liter of culture solution is 0.02mol, and the content of sodium bicarbonate is 0.1mol.

[0062] (1) The primary culture of Monospina

[0063] Inoculate the single needle algae species into a 2000mL Erlenmeyer flask filled with 1000mL culture fluid, and the OD of the culture medium after inoculating the single needle algae species 680 The value is 0.015. Cultiv...

Embodiment 2

[0069] This example is used to illustrate the method of cultivating Monospora using an open photobioreactor.

[0070] In this example, the cultivation of Monostipella biomass growth stage and the cultivation of oil accumulation stage were all carried out in the photobioreactor of the open racetrack pool. Among them, there is one open raceway pond photobioreactor cultivated in the biomass growth stage, one open racetrack pond photobioreactor cultivated in the oil accumulation stage, and one separation and storage device.

[0071] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium. The content of sodium nitrate in each liter of culture solution is 0.02mol, and the content of sodium bicarbonate is 0.1mol.

[0072] (1) The primary culture of Monospina

[0073] Inoculate the single needle algae species into a 2000mL Erlenmeyer flask filled with 1000mL culture fluid, and the OD of the culture medium after inoculating the single needle algae speci...

Embodiment 3

[0079] This example is used to illustrate the method of cultivating Monostipella using a composite photobioreactor.

[0080] In this example, the cultivation of Monostipella biomass growth stage was carried out in a closed photobioreactor, and the cultivation of oil accumulation stage was carried out in an open raceway pool photobioreactor. Among them, there are 10 closed photobioreactors cultivated in the biomass growth stage, 1 open track pool photobioreactor cultivated in the oil accumulation stage, and 1 separation and storage device.

[0081] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium. The content of sodium nitrate in each liter of culture solution is 0.02mol, and the content of sodium bicarbonate is 0.1mol.

[0082] (1) The primary culture of Monospina

[0083] Inoculate the single needle algae species into a 2000mL Erlenmeyer flask filled with 1000mL culture fluid, and the OD of the culture medium after inoculating the single...

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Abstract

The invention relates to the field of microalgae biology, and discloses a culture method for monoraphidium. The method includes (1) subjecting the monoraphidium to culture of a biomass increasing stage; (2) when the OD680 value of an alga solution is 0.01-10, separating the alga solution to obtain a separated clear liquid I and monoraphidium mud, and returning the separated clear liquid I to the step (1) for reuse; (3) subjecting the monoraphidium mud obtained in the step (2) to culture of an oil and grease accumulating stage, with the culture manner being nitrogen starvation stress culture; (4) separating after 5-10 days of stress culture to obtain a separated clear liquid II and monoraphidium with accumulated oil and grease, and returning the separated clear liquid II to the step (3) for reuse; and (5) drying the monoraphidium with accumulated oil and grease to obtain a monoraphidium product. According to the method, operation of a culture process is stable, quality control is easy, and all culture liquids in the culture process can be recovered and reused so that the culture cost is low and no environment pollution is generated.

Description

technical field [0001] The invention relates to the field of microalgae biology, in particular to a method for cultivating Monopodella. Background technique [0002] Monoraphidium is a single-celled green alga with an irregular spindle shape. The cells are 12-26 μm long and 4-8 μm wide. They are easy to reproduce in fresh water and consume a certain amount of biomass while accumulating biomass. Carbon dioxide is beneficial to the purification and protection of the environment. In addition, the biomass in Monopodella is rich in oil, and the oil content can reach about 30% of the dry cell weight, which can be used as a potential source of bioenergy. Simultaneously, Monopodella is also rich in β-carotene, vitamins and carbohydrates, which has high added value. Therefore, the large-scale cultivation of Monospora is of great significance. However, the research on large-scale culture and production technology of Monospora is still very little. [0003] Among them, patent appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 刘伟孙春燕周旭华
Owner CHINA PETROLEUM & CHEM CORP
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