Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of cultivation method of monospora

A breeding method, the technology of Monospina, which is applied in the cultivation field of Monospina, can solve the problems of high oil content and low breeding cost of Monospina, and achieve the effects of high oil content, low breeding cost and easy quality control

Active Publication Date: 2020-05-19
CHINA PETROLEUM & CHEM CORP +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned defects in the prior art, and to provide a method for cultivating Monospina, the method can realize the continuous cultivation of Monospina, the operation of the cultivation process is stable, the quality control is easy, and the oil content of Monospina is low. High, and all the culture fluid can be recycled during the cultivation process, the cultivation cost is low and there will be no problems of waste liquid discharge and environmental pollution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] This example is used to illustrate the method of cultivating Monospora using a closed photobioreactor.

[0059] In this example, the cultivation of Monostipella biomass growth stage and the cultivation of oil accumulation stage were all carried out in a closed photobioreactor. Among them, there is one closed photobioreactor for cultivation in the biomass growth stage, one closed photobioreactor for cultivation in the oil accumulation stage, and one separation and storage device.

[0060] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium. The content of sodium nitrate in each liter of culture solution is 0.02mol, and the content of sodium bicarbonate is 0.1mol.

[0061] (1) The primary culture of Monospina

[0062] Inoculate the single needle algae species into a 2000mL Erlenmeyer flask filled with 1000mL culture fluid, and the OD of the culture medium after inoculating the single needle algae species 680 The value is 0.015. Cultiv...

Embodiment 2

[0069] This example is used to illustrate the method of cultivating Monospora using an open photobioreactor.

[0070] In this example, the cultivation of Monostipella biomass growth stage and the cultivation of oil accumulation stage were all carried out in the photobioreactor of the open racetrack pool. Among them, there is one open raceway pond photobioreactor cultivated in the biomass growth stage, one open racetrack pond photobioreactor cultivated in the oil accumulation stage, and one separation and storage device.

[0071] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium. The content of sodium nitrate in each liter of culture solution is 0.02mol, and the content of sodium bicarbonate is 0.1mol.

[0072] (1) The primary culture of Monospina

[0073] Inoculate the single needle algae species into a 2000mL Erlenmeyer flask filled with 1000mL culture fluid, and the OD of the culture medium after inoculating the single needle algae speci...

Embodiment 3

[0080] This example is used to illustrate the method of cultivating Monostipella using a composite photobioreactor.

[0081] In this example, the cultivation of Monostipella biomass growth stage was carried out in a closed photobioreactor, and the cultivation of oil accumulation stage was carried out in an open raceway pool photobioreactor. Among them, there are 10 closed photobioreactors cultivated in the biomass growth stage, 1 open track pool photobioreactor cultivated in the oil accumulation stage, and 1 separation and storage device.

[0082] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium. The content of sodium nitrate in each liter of culture solution is 0.02mol, and the content of sodium bicarbonate is 0.1mol.

[0083] (1) The primary culture of Monospina

[0084] Inoculate the single needle algae species into a 2000mL Erlenmeyer flask filled with 1000mL culture fluid, and the OD of the culture medium after inoculating the single...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to the field of microalgae organisms, and discloses a monoraphidium breeding method, which comprises: (1) carrying out biomass increasing stage culture on monoraphidium; (2) separating the monoraphidium liquid to obtain a separated clear liquid I and the monoraphidium mud when the OD680 value of the cultured monoraphidium liquid is 0.01-10, and returning the separated clear liquid to the step (1) so as to be reused; (3) carrying out grease accumulation stage stress culture on the monoraphidium mud obtained in the step (2); (4) separating after carrying out the stress culture for 5-10 days to obtain a separated clear liquid II and the grease-accumulating monoraphidium, and returning the separated clear liquid II to the step (3) so as to be reused; and (5) drying the grease-accumulating monoraphidium to obtain the monoraphidium product. According to the present invention, the operations during the breeding process are stable, the quality control is easy, the grease content of the monoraphidium is high, all the culture liquid during the breeding process can be recycled, the breeding cost is low, and the problems of waste liquid emission and environmental pollution cannot be produced.

Description

technical field [0001] The invention relates to the field of microalgae biology, in particular to a method for cultivating Monopodella. Background technique [0002] Monoraphidium is a single-celled green alga with an irregular spindle shape. The cells are 12-26 μm long and 4-8 μm wide. They are easy to reproduce in fresh water and consume a certain amount of biomass while accumulating biomass. Carbon dioxide is beneficial to the purification and protection of the environment. In addition, the biomass in Monopodella is rich in oil, and the oil content can reach about 30% of the dry cell weight, which can be used as a potential source of bioenergy. Simultaneously, Monopodella is also rich in β-carotene, vitamins and carbohydrates, which has high added value. Therefore, the large-scale cultivation of Monospora is of great significance. However, the research on large-scale culture and production technology of Monospora is still very little. [0003] Among them, patent appli...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 刘伟孙春燕周旭华
Owner CHINA PETROLEUM & CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com