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A culture method for monoraphidium

A method for culturing and a technique for monocystis algae, applied in the field of cultivating monocythium algae, can solve the problems of high oil content and low cultivation cost of monocythium algae, and achieve the effects of high oil content, low cultivation cost and easy quality control.

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the above-mentioned defects in the prior art, and to provide a method for cultivating Monospina, the method can realize the continuous cultivation of Monospina, the operation of the cultivation process is stable, the quality control is easy, and the oil content of Monospina is low. High, and all the culture fluid can be recycled during the cultivation process, the cultivation cost is low and there will be no problems of waste liquid discharge and environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] This embodiment is used to illustrate the method of cultivating monophyta in a closed photobioreactor.

[0060] In this embodiment, the cultivation of the biomass growth stage and the culturing of the oil accumulation stage of the monophyllum are both performed in a closed photobioreactor. Among them, there is one closed photobioreactor cultivated in the biomass growth stage, one closed photobioreactor cultivated in the fat accumulation stage, and one separation, storage and transportation device.

[0061] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium, and the content of sodium nitrate per liter of culture solution is 0.02 mol, and the content of sodium bicarbonate is 0.1 mol.

[0062] (1) Primary culture of Monophyta

[0063] Inoculate the mononeedle species into a 2000mL Erlenmeyer flask containing 1000mL of culture solution, and the OD of the culture solution after inoculation of the mononeedle species 680 The value is 0.015. Cultiva...

Embodiment 2

[0069] This embodiment is used to illustrate the method of cultivating monophytes using an open photobioreactor.

[0070] In the present embodiment, the cultivation of the biomass growth stage and the culturing of the oil accumulation stage of the monophyllum are both carried out in the open track pond photobioreactor. Among them, there is one open track pond photobioreactor cultivated in the biomass growth stage, one open track pond photobioreactor cultivated in the oil accumulation stage, and one separation, storage and transportation device.

[0071] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium, and the content of sodium nitrate per liter of culture solution is 0.02 mol, and the content of sodium bicarbonate is 0.1 mol.

[0072] (1) Primary culture of Monophyta

[0073] Inoculate the mononeedle species into a 2000mL Erlenmeyer flask containing 1000mL of culture solution, and the OD of the culture solution after inoculation of the mononeedle...

Embodiment 3

[0079] This embodiment is used to illustrate the method of cultivating mononeedle algae using a composite photobioreactor.

[0080] In this embodiment, the cultivation of the growth stage of the monophytaceae biomass is carried out in a closed photobioreactor, and the cultivation of the oil accumulation stage is carried out in an open raceway pond photobioreactor. Among them, there are 10 closed photobioreactors cultivated in the biomass growth stage, one open track pond photobioreactor cultivated in the oil accumulation stage, and one separation, storage and transportation device.

[0081] The culture solution is composed of sodium nitrate, sodium bicarbonate and BG11 medium, and the content of sodium nitrate per liter of culture solution is 0.02 mol, and the content of sodium bicarbonate is 0.1 mol.

[0082] (1) Primary culture of Monophyta

[0083] Inoculate the mononeedle species into a 2000mL Erlenmeyer flask containing 1000mL of culture solution, and the OD of the culture soluti...

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Abstract

The invention relates to the field of microalgae biology, and discloses a culture method for monoraphidium. The method includes (1) subjecting the monoraphidium to culture of a biomass increasing stage; (2) when the OD680 value of an alga solution is 0.01-10, separating the alga solution to obtain a separated clear liquid I and monoraphidium mud, and returning the separated clear liquid I to the step (1) for reuse; (3) subjecting the monoraphidium mud obtained in the step (2) to culture of an oil and grease accumulating stage, with the culture manner being nitrogen starvation stress culture; (4) separating after 5-10 days of stress culture to obtain a separated clear liquid II and monoraphidium with accumulated oil and grease, and returning the separated clear liquid II to the step (3) for reuse; and (5) drying the monoraphidium with accumulated oil and grease to obtain a monoraphidium product. According to the method, operation of a culture process is stable, quality control is easy, and all culture liquids in the culture process can be recovered and reused so that the culture cost is low and no environment pollution is generated.

Description

Technical field [0001] The present invention relates to the field of microalgae biology, in particular, to a method for cultivating single needle algae. Background technique [0002] Monoraphidium is a single-celled green algae with an irregular spindle shape. The cells are 12-26μm long and 4-8μm wide. They are easy to multiply in fresh water and consume a certain amount of biomass while accumulating biomass. Carbon dioxide is beneficial to the purification and protection of the environment. In addition, the biomass in the monophyllum is rich in oil, and the oil content can reach about 30% of the dry cell weight, which can be used as a potential source of bioenergy. At the same time, the single needle algae is also rich in β-carotene, vitamins and carbohydrates, which has a high added value. Therefore, the large-scale cultivation of monophyta is of great significance. However, the research on the large-scale cultivation and production technology of Monophyta is still very few....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 刘伟孙春燕周旭华
Owner CHINA PETROLEUM & CHEM CORP
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