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Monoraphidium sp. strain with high yield of starch and fat as well as culture and applications of monoraphidium sp.

A single needle algae and starch technology, which is applied in the fields of biotechnology and bioenergy, can solve the problems of single needle algae product and narrow application range, and achieve the effect of good application potential, high economic value and high yield.

Active Publication Date: 2017-03-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the shortcomings of the existing single needle algae products and narrow application range, the present invention provides a high starch and oil yield single needle algae and its cultivation application

Method used

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  • Monoraphidium sp. strain with high yield of starch and fat as well as culture and applications of monoraphidium sp.
  • Monoraphidium sp. strain with high yield of starch and fat as well as culture and applications of monoraphidium sp.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Separation and screening to obtain Monostipella SS-06

[0022] (1) Enrichment culture: In October 2011, water samples were collected from Longhu Lake in Shoushan Leisure Resort, Dubolte Mongolian Autonomous County, Heilongjiang. After filtering with gauze, 100mL of water samples were inoculated into 200mL of BG11 medium for enrichment For cultivation, the light intensity was 7000lux, the temperature was 25°C, the light-dark cycle was 24h, and the light-dark time ratio was 14:10. After about half a month of cultivation, the culture medium was obviously green, and a large number of algae were observed under the microscope. The formulation of the BG11 medium is shown in Table 1 and Table 2.

[0023] Table 1 BG11 medium

[0024]

[0025] *Table 2 Composition of A5+Co solution in Table 1

[0026]

[0027] (2) Obtaining pure algae strains: Dilute the enriched water sample to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultiva...

Embodiment 2

[0029] Example 2 Identification of algae strains

[0030] The DNA of the obtained SS-06 algae cells was extracted by CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Monoraphidium sp. It has the greatest similarity, its BLASTn value is 2591, and its Max index value is 99%, so it can be determined that SS-06 is Monospirulina ( Monoraphidium sp. ).

Embodiment 3

[0031] Example 3 Cultivation and application of Monospina SS-06

[0032] Single needle algae SS-06 was cultivated in a tubular light reactor at a culture temperature of 15°C, pH 6.0, light intensity of 5000lux, ventilation rate of 0.25vvm, CO 2 The content is 3v%. After 7 days of continuous light culture, the algae liquid is collected by centrifugation, and after freeze-drying, the biomass of microalgae is 2.0g / L, the protein content is 20%, the oil content is 22%, and the starch content is 11%.

[0033] The protein detection method was determined by Bradford method. The oil detection method is to weigh the dried algae powder, put it into a porcelain mortar, add appropriate amount of quartz sand and liquid nitrogen, grind and break the wall after the liquid nitrogen volatilizes; add 10mL ethyl acetate:n-hexane = 1:1 mixed organic solvent, treated with ultrasonic cleaner for 20 minutes, extracted in 50-60°C constant temperature water bath for 45 minutes; centrifuged for 15 minu...

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PUM

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Abstract

The invention discloses a monoraphidium sp. strain SS-06 with high yield of starch and fat, wherein the classification name of the monoraphidium sp. strain SS-06 is Monoraphidium sp., and the monoraphidium sp. strain SS-06 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 24, 2015, and is assigned with the accession number of CGMCC No.10765. The bred monoraphidium sp. strain SS-06 has good application potential, is wide in environmental adaptation, and can produce starch and fat with high yield under the suitable condition, therefore, the different demands of the downstream production can be satisfied, and the application range of the monoraphidium sp. strain SS-06 is expanded.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a high-yield starch and oil-yielding monospora and its cultivation application. Background technique [0002] Due to the depletion of world oil resources and gradually rising prices, increasingly severe environmental protection issues, and a series of impacts caused by global climate change, since the 1970s, many countries have paid more and more attention to the development of bio-energy and bio-based chemicals. Among the many biomass energy and biomass chemicals, microalgae has received more and more attention due to its advantages of "not competing with others for food and land". [0003] Algae is one of the most primitive organisms with a wide distribution. Most algae live in water. They have a simple structure and have the characteristics of high photosynthetic efficiency, short growth cycle and fast speed. Algae include microalgae (single-celled euka...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P19/04C12P7/64C12R1/89
Inventor 廖莎王鹏翔孙启梅师文静李晓姝樊亚超张霖高大成王领民乔凯
Owner CHINA PETROLEUM & CHEM CORP
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