Method for improving oil content of oil-producing microalgae based on fulvic acid

A technology for oil content, oil-producing microalgae, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of small molecular weight, growth promotion and oil accumulation, and high functional group content

Inactive Publication Date: 2016-02-03
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few reports on the effects of fulv

Method used

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  • Method for improving oil content of oil-producing microalgae based on fulvic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Fulvic acid improves biomass and oil production experiment in BG-11 medium, comprises the following steps:

[0027] (1) Select the BG-11 medium (China Freshwater Algae Species Bank) as the basic medium of Monostifles, put it in a 500mL triangular flask, add glucose in an amount of 10g / L as a carbon source, and add fulvic acid Dissolved in ultrapure water and configured as a mother solution of 1000mg / L, the concentration of fulvic acid in the medium is 20mg / L, and the pH value is adjusted to 6.8.

[0028] (2) High-pressure high-temperature sterilization for 20 minutes, inserting Monopodella oleaginum, and controlling the cell density of the algae at 10 6 cells / mL, cultured in shake flasks, the culture temperature was 23°C, the shaker speed was 130r / min, and cultured in the dark.

[0029] (3) The final culture medium of each group was enriched by centrifugation (3000r / min, 6min), washed twice with distilled water, freeze-dried and weighed. Add 2 times the mass of freeze...

Embodiment 2

[0031] Fulvic acid improves biomass and oil production experiment in BG-11 medium, comprises the following steps:

[0032] (1) Select the BG-11 medium (China Freshwater Algae Species Bank) as the basic medium of Monostifles, put it in a 500mL triangular flask, add glucose in an amount of 10g / L as a carbon source, and add fulvic acid Dissolved in ultrapure water and configured as a mother solution of 1000mg / L, the concentration of fulvic acid in the medium is 40mg / L, and the pH value is adjusted to 6.9.

[0033] (2) High-pressure high-temperature sterilization for 20 minutes, inserting Monopodella oleaginum, and controlling the cell density of the algae at 10 6 cells / mL, cultured in shake flasks, the culture temperature was 24°C, the shaker speed was 140r / min, and cultured in the dark.

[0034] (3) The final culture medium of each group was enriched by centrifugation (3000r / min, 6min), washed twice with distilled water, freeze-dried and weighed. Add 2 times the mass of freeze...

Embodiment 3

[0036] Fulvic acid improves biomass and oil production experiment in BG-11 medium, comprises the following steps:

[0037] (1) Select the BG-11 medium (China Freshwater Algae Species Bank) as the basic medium of Monostifles, put it in a 500mL triangular flask, add glucose in an amount of 10g / L as a carbon source, and add fulvic acid Dissolved in ultrapure water and configured as a mother solution of 1000mg / L, the concentration of fulvic acid in the medium is 60mg / L, and the pH value is adjusted to 7.

[0038] (2) High-pressure high-temperature sterilization for 20 minutes, inserting Monopodella oleaginum, and controlling the cell density of the algae at 10 6 cells / mL, cultured in shake flasks, the culture temperature was 25°C, the shaker speed was 150r / min, and cultured in the dark.

[0039] (3) The final culture medium of each group was enriched by centrifugation (3000r / min, 6min), washed twice with distilled water, freeze-dried and weighed. Add 2 times the mass of freeze-d...

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Abstract

The invention discloses a method for improving oil content of oil-producing microalgae based on fulvic acid. The method comprises the steps of preparing a culture medium based on fulvic acid; placing oil-producing monoraphidium in the culture medium to perform shaking culture; and preparing biodiesel. The fulvic acid improves microalgae growth rate and oil content, and the method has the advantages of being low in investment, simple to operate and high in efficiency. The method is favorable for urging the microalgae to absorb more nutrient more quickly and promoting development and growth of the microalgae. The activity of multiple synthetase is improved, the culture time of the microalgae is shortened, the oil content is greatly improved, and the method is of great significance to microalgae biodiesel production industrialization.

Description

technical field [0001] The invention belongs to the technical field of biodiesel, and in particular relates to a method for increasing the oil content of oil-producing microalgae based on fulvic acid. Background technique [0002] Biodiesel refers to methyl ester or ethyl ester fuel made from vegetable oil, animal oil, microbial oil, waste cooking oil, etc. through transesterification process. Compared with conventional diesel, it has the characteristics of renewable, easy to degrade, low pollutant emission after combustion, and low greenhouse gas emission. [0003] Microalgae have many advantages as raw materials for biodiesel production, such as simple cell structure; high photosynthetic efficiency, high biological output, fast growth and reproduction, and short growth cycle; and high oil content; low requirements for growth conditions, can be used in Survive under extreme conditions; and microalgae do not contain sulfur, non-toxic and degradable. Therefore, the use of m...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCY02E50/10
Inventor 余旭亚丁柯张和平赵永腾尚敏敏车绕琼
Owner KUNMING UNIV OF SCI & TECH
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