Method for preparing microalgae oil
A technology of microalgae oil and microalgae, which is applied in the fields of biotechnology and bioenergy, can solve the problems of unfavorable algae absorption and utilization, non-use, affecting the growth of algae cells, etc., so as to alleviate the greenhouse effect and exhaust gas pollution problems, and improve tolerance. and carbon sequestration efficiency, the effect of inhibiting the growth of miscellaneous bacteria
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Embodiment 1
[0019] The preparation of embodiment 1 microalgae seed liquid
[0020] BG11 medium was used for microalgae culture, and the medium formulations are shown in Table 1 and Table 2.
[0021] Table 1BG11 medium
[0022]
[0023] * Composition of A5+Cosolution in Table 2 and Table 1
[0024]
[0025] Scenedesmus obliquus FSH-Y2 and Monostipella SS-B1 were respectively inoculated in BG11 liquid culture solution, and the pH of the culture medium for cultivating Scenedesmus obliquus FSH-Y2 was adjusted to 10-12. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture to the logarithmic growth phase, and Scenedesmus obliquus FSH-Y2 is obtained Seed solution and Monostipella SS-B1 seed solution, the above seed solution was stored at 15°C under weak light for later use.
[0026] The above-mentioned Scenedesmus obliquus FSH-Y2 seed liquid, M...
Embodiment 2
[0027] The preparation of embodiment 2 microalgae oil
[0028] The different seed solutions prepared in Example 1 were respectively inoculated in BG11 medium to prepare microalgae oil. In a photobioreactor, the seed liquid of Scenedesmus obliquus FSH-Y2, the seed liquid of Monocystus SS-B1 and the mixed microalgae seed liquid mixed at a volume ratio of 1:1 were inoculated to BG11 according to the inoculum amount of 10v%. In the culture medium, the pH value is controlled between 9 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the culture temperature is 28°C, the light cycle is 24h, and the light intensity is 28°C. The dark time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculat...
Embodiment 3
[0032] Embodiment 3 Utilizes flue gas to prepare microalgae oil
[0033] The preparation conditions are the same as in Example 1, except that the 2 and CO 2 flue gas, CO in the flue gas 2 The content of SO is 10v%~30v%, SO 2 The content is 200×10 -6 ~400×10 -6 (v / v), the pH of the reaction system was maintained at 10. After 10 days, the culture was terminated, the algal cells were collected by centrifugation, and the dry weight of the algal powder was measured after vacuum freeze-drying at -60°C to a constant weight, and the biomass production was calculated, and the Normal hexane: ethyl acetate method records total lipid content, and the results are shown in table 4.
[0034] Table 4 The cultivation effect comparison of mixed algae and single algae
[0035]
[0036] It can be seen from Table 4 that in a high pH system, compared with single algae, mixed algae can not only tolerate high concentrations of CO 2 , and can tolerate a certain concentration of SO 2 , resul...
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