Method for preparing microalgae oil

A technology of microalgae oil and microalgae, which is applied in the fields of biotechnology and bioenergy, can solve the problems of unfavorable algae absorption and utilization, non-use, affecting the growth of algae cells, etc., so as to alleviate the greenhouse effect and exhaust gas pollution problems, and improve tolerance. and carbon sequestration efficiency, the effect of inhibiting the growth of miscellaneous bacteria

Active Publication Date: 2016-06-29
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
The above patents either cannot efficiently utilize CO 2 Production of oils or fats, or obtaining biomass with insufficient oil content
Especially in practical applications, when the environment CO 2 When the volume fraction is greater than 5%, the growth of most microalgae will be inhibited, which will affect the carbon sequestration efficiency
At the same time, general microalgae are suitable for growth under neutral conditions, and it is not conducive to the growth of microalgae under acidic or alkaline conditions, while CO 2 Low solubility in neutral environment, not conducive to algae absorption and utilization
[0005] Liu Pinghuai et al. (The Effect of Organic Carbon Sources on Cell Growth, Oil Accumulation and Photosynthesis of Monospina, Bioengineering, 2012, 33(18):224-246) introduced a production method of using organic carbon sources to cultivate Monostipola , although the biomass exceeds 10g / L at the end of the culture, this method is a heterotrophic culture method of Monostipella. During the culture process, organic carbon sources such as glucose are used to achieve cell growth. This culture method does not use CO 2 Other inorganic carbon sources are economical, and the addition of organic carbon sources is prone to bacterial contamination during the cultivation process, which affects the growth of algae cells

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  • Method for preparing microalgae oil
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  • Method for preparing microalgae oil

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Experimental program
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Effect test

Embodiment 1

[0019] The preparation of embodiment 1 microalgae seed liquid

[0020] BG11 medium was used for microalgae culture, and the medium formulations are shown in Table 1 and Table 2.

[0021] Table 1BG11 medium

[0022]

[0023] * Composition of A5+Cosolution in Table 2 and Table 1

[0024]

[0025] Scenedesmus obliquus FSH-Y2 and Monostipella SS-B1 were respectively inoculated in BG11 liquid culture solution, and the pH of the culture medium for cultivating Scenedesmus obliquus FSH-Y2 was adjusted to 10-12. Cultivate in a constant temperature light shaker, the culture temperature is 25°C, the light cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 5000Lux, 120rpm shaking culture to the logarithmic growth phase, and Scenedesmus obliquus FSH-Y2 is obtained Seed solution and Monostipella SS-B1 seed solution, the above seed solution was stored at 15°C under weak light for later use.

[0026] The above-mentioned Scenedesmus obliquus FSH-Y2 seed liquid, M...

Embodiment 2

[0027] The preparation of embodiment 2 microalgae oil

[0028] The different seed solutions prepared in Example 1 were respectively inoculated in BG11 medium to prepare microalgae oil. In a photobioreactor, the seed liquid of Scenedesmus obliquus FSH-Y2, the seed liquid of Monocystus SS-B1 and the mixed microalgae seed liquid mixed at a volume ratio of 1:1 were inoculated to BG11 according to the inoculum amount of 10v%. In the culture medium, the pH value is controlled between 9 and 11, and a mixed gas of nitrogen and carbon dioxide is introduced, the carbon dioxide content is 5v% to 45v%, the light intensity is 5000Lux, the culture temperature is 28°C, the light cycle is 24h, and the light intensity is 28°C. The dark time ratio is 14:10. After cultivating for 7 days, it is in a stable period. After cultivating, the algae liquid is collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder is measured to calculat...

Embodiment 3

[0032] Embodiment 3 Utilizes flue gas to prepare microalgae oil

[0033] The preparation conditions are the same as in Example 1, except that the 2 and CO 2 flue gas, CO in the flue gas 2 The content of SO is 10v%~30v%, SO 2 The content is 200×10 -6 ~400×10 -6 (v / v), the pH of the reaction system was maintained at 10. After 10 days, the culture was terminated, the algal cells were collected by centrifugation, and the dry weight of the algal powder was measured after vacuum freeze-drying at -60°C to a constant weight, and the biomass production was calculated, and the Normal hexane: ethyl acetate method records total lipid content, and the results are shown in table 4.

[0034] Table 4 The cultivation effect comparison of mixed algae and single algae

[0035]

[0036] It can be seen from Table 4 that in a high pH system, compared with single algae, mixed algae can not only tolerate high concentrations of CO 2 , and can tolerate a certain concentration of SO 2 , resul...

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Abstract

The invention discloses a method for preparing microalgae oil. The method comprises the following steps: adding a microalgae culture medium and a mixed microalgae seed solution to a photobioreactor, keeping the pH value of a culture system to be 8-12 and introducing gas which contains 5-45v% of CO2 by volume, wherein the mixed microalgae includes scenedesmus obliqnus FSH-Y2 and monoraphidium sp SS-B1 which are preserved in China General Microbiological Culture Collection Center on September 11, 2012 and April 15, 2013 by preservation numbers of CGMCC No.6551 and CGMCC No.7479, respectively. By virtue of the method disclosed by the invention, the solubility and the tolerance of the microalgae culture system to the high-concentration CO2 are improved, a carbon sequestration efficiency is improved and the yield of the microalgae oil is obviously improved, and the obtained microalgae oil can be used for producing biodiesel.

Description

technical field [0001] The invention belongs to the field of biotechnology and bioenergy, in particular to a 2 Method for preparing microalgae oil. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/64C12R1/89
CPCY02E50/10
Inventor 师文静姚新武廖莎孙启梅樊亚超高大成王领民李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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