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Method for producing microalgae grease by smoke

A technology of microalgae oil and flue gas, which is applied in the fields of biotechnology and bioenergy, can solve the problems of reducing carbon fixation efficiency, inhibiting microalgae growth, and insufficient oil content, so as to improve tolerance and solubility, and improve microalgae Oil content, effect of increasing oil content

Active Publication Date: 2020-05-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
But in practical application, when CO 2 When the concentration is higher than 5%, the growth of most microalgae will be inhibited
Moreover, the solubility of carbon dioxide has a certain relationship with the acidity and alkalinity of the solution. The solubility of carbon dioxide increases significantly in alkaline solutions, and most microalgae do not have the ability to tolerate high pH values, thus limiting the solubility of carbon dioxide , affecting the fixation efficiency of carbon dioxide by algal strains
[0006] The above existing technologies either cannot efficiently utilize CO 2 Production of oils or fats, or obtaining biomass with insufficient oil content
Especially in practical applications, when the environment CO 2 When the volume fraction is greater than 5v%, the growth of most microalgae will be inhibited, and the carbon fixation efficiency will be low; at the same time, general microalgae are suitable for growth under neutral conditions, and it is not conducive to the growth of microalgae under acidic or alkaline conditions , while microalgae use CO 2 Usually HCO dissolved in water 3 - It exists in the form of ions, and the solubility of carbon dioxide is low in a neutral environment, which is not conducive to the absorption and utilization of algae
Moreover, if the fossil fuel waste gas passed through contains high concentrations of SOx, NOx and other gases, it will also inhibit the growth of microalgae and reduce the efficiency of carbon fixation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) In a 20L photobioreactor, add Chlorella kessleri FSH-Y3 seed solution and microalgae medium. The amount of seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 10. It is 8L, the culture temperature is 25℃, the light-dark reaction cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 8000Lux, and CO in the flue gas is introduced. 2 The content is 5v%, and the NO content is 50×10 -6 (v / v), SO 2 The content is 60×10 -6 (v / v).

[0040] (2) After culturing for 2 days, adjust the pH of the culture system to 8, insert 400 mL of cellulite SS-B7 seed solution, and 400 mL of monocystis SS-B1 seed solution, the culture temperature is 25°C, the continuous light culture is performed, and the light intensity is 8000Lux. , into the CO in the flue gas 2 The content is 40v%, and the NO content is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0041] (3) After cultivating for 5 days, 800 mL of the seed solution of ...

Embodiment 2

[0044] (1) In a 20L photobioreactor, add Chlorella kessleri FSH-Y3 seed solution and microalgae medium. The amount of seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 12. It is 8L, the culture temperature is 30°C, the light-dark reaction cycle is 24h, the light-dark time ratio is 14:10, the light intensity is 15000Lux, and CO in the flue gas is introduced. 2 The content is 5v%, and the NO content is 50×10 -6 (v / v), SO 2 The content is 60×10 -6 (v / v).

[0045] (2) After culturing for 2 days, adjust the pH of the culture system to 9, insert 400 mL of cellulite SS-B7 seed solution, and 400 mL of monocystis SS-B1 seed solution, the cultivation temperature is 30 °C, the continuous light cultivation is performed, and the light intensity is 15000Lux. , into the CO in the flue gas 2 The content is 40v%, and the NO content is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0046] (3) After cultivating for 5 days, 800 mL of the seed ...

Embodiment 3

[0049] (1) In a 20L photobioreactor, add Chlorella kessleri FSH-Y3 seed solution and microalgae medium. The amount of seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 11. It is 8L, the culture temperature is 20°C, the light-dark reaction cycle is 24h, the light-dark time ratio is 10:14, the light intensity is 5000Lux, and CO in the flue gas is introduced. 2 The content is 5v%, and the NO content is 50×10 -6 (v / v), SO 2 The content is 60×10 -6 (v / v).

[0050] (2) After culturing for 2 days, adjust the pH of the culture system to 10, insert 400 mL of cellulite SS-B7 seed solution, and 400 mL of monocystis SS-B1 seed solution, the cultivation temperature is 20 °C, the continuous light cultivation, the light intensity is 5000Lux , into the CO in the flue gas 2 The content is 40v%, and the NO content is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0051] (3) After cultivating for 5 days, 800 mL of the seed solution of the sin...

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Abstract

The invention relates to a method for producing microalgae grease by smoke. A culture medium and a scenedesmus obliquus FSH-Y2 or / and Parachlorella kessleri FSH-Y3 seed solution are added into a photobioreactor to regulate pH to be 10-12, and light and dark alternation culture is carried out for a period of time; the pH is regulated to be 8-10, an Ankistrodesmus sp. SS-B7 seed solution inoculated, meanwhile, a Desmodesmus sp. MH-04 or / and MonorapHidium sp SS-B1 seed solution is inoculated, and illumination culture is continued for a period of time; and then, a MonorapHidium sp SHJ-02 or / andDesmodesmus sp. HCS-02 seed solution is inoculated, and light and dark alternation culture is carried out to a stable period under low illumination intensity so as to harvest microalgae cells. According to the method, the smoke is used for producing the microalgae grease, while hybrid bacterium pollution is inhibited, the tolerance and the solubleness of a microalgae culture system for high-concentration CO2 can be improved, carbon fixation efficiency is improved, and a harvest yield of the microalgae grease is obviously improved.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for producing microalgae oil by utilizing flue gas. Background technique [0002] Due to the increasing reduction of fossil energy and the increase of the greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forest biomass, crops, aquatic plants, agricultural wastes, etc. Among many biomass energy sources, microalgae are important renewable resources. They have the characteristics of wide distribution, large biomass, high photosynthesis efficiency, strong environmental adaptability, short growth cycle and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can rea...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/64Y02P20/59
Inventor 师文静张霖樊亚超李晓姝廖莎
Owner CHINA PETROLEUM & CHEM CORP
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