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Wall-breaking and surfactant-assisted method used for extracting grease from microalgae containing oil

A surfactant and microalgae technology, applied in the direction of fat oil/fat production, fat production, etc., can solve the problems of destroying the original liquid components in cells, large amount of organic solvents, and low oil extraction rate, so as to improve the wall breaking effect and oil The effect of high extraction rate and reduced dosage

Active Publication Date: 2016-01-27
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the low oil extraction rate in existing oil-containing microalgae wall breaking and oil extraction, easy to destroy the original liquid components in cells, harsh reaction conditions, large amount of organic solvents, serious environmental pollution, high energy consumption or narrow application range, etc. Disadvantages, provide a new method for breaking the wall of oil-containing microalgae and extracting its oil

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and extracting oil with the aid of surfactant of the present invention.

[0055] (1) Put 5 g of Nannochloropsis dry powder into a conical flask, add water to prepare a 25% by weight suspension, adjust the pH value to 8.5, and stir at 80° C. for 4 hours;

[0056] (2) Regulate the pH value to 5. In terms of Nannochloropsis dry powder, cellulase (Beijing Hongrun Baoshun Technology Co., Ltd., 2000IU / g, the same below), pectinase ( Beijing Hongrun Baoshun Technology Co., Ltd., 20000IU / g, the same below), acid protease (Beijing Hongrun Baoshun Technology Co., Ltd., 50000IU / g, the same below) and lysozyme (Sinopharm Chemical Reagent Co., Ltd., 20000IU / g , the same below), reacted at 50°C for 5h;

[0057] (3) With respect to the suspension of Nannochloropsis, add according to the amount of 200mg / L and and The molar ratio is 1:5, stir and react at 50°C for 2h, then add 20ml of n-...

Embodiment 2

[0059] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and extracting oil with the aid of surfactant of the present invention.

[0060] (1) Put 5g of chlorella dry powder into a conical flask, add water to prepare a 15% by weight suspension, adjust the pH value to 10, and stir at 60°C for 6h;

[0061] (2) Adjust the pH value to 4, add cellulase, pectinase, acid protease and lysozyme in an amount of 200 IU / g, and react at 40°C for 6 hours;

[0062] (3) With respect to the suspension of chlorella, add according to the amount of 100mg / L and and and The molar ratio is 1:4, stirred and reacted at 40°C for 2.5h, then added 10ml of chloroform, stirred for 25min, centrifuged at 4000rpm for 15min, took the organic solvent layer containing oil on it, and then 100-200Pa, 60°C Distilled under reduced pressure, dried at constant weight to obtain oil, the extraction rate of oil is shown in Table 1.

Embodiment 3

[0064] This example is used to illustrate the method of breaking the wall of oleaginous microalgae and extracting oil with the aid of surfactant of the present invention.

[0065] (1) Put 5 g of Scenedesmus dry powder into a conical flask, add water to prepare a 30% by weight suspension, adjust the pH to 12, and stir at 100°C for 2 hours;

[0066] (2) Adjust the pH value to 5.5, add cellulase, pectinase, acid protease and lysozyme in an amount of 300 IU / g based on Scenedesmus dry powder, and react at 60°C for 4 hours;

[0067] (3) With respect to the suspension of Scenedesmus, add according to the amount of 400mg / L and and The molar ratio is 1:6, stirred and reacted at 60°C for 1.5h, then added 50ml of petroleum ether (Beijing Chemical Plant, analytically pure), stirred for 35min, centrifuged at 6000rpm for 5min, took the organic solvent layer containing oil above, and then 100-200Pa, distilled under reduced pressure at 50°C, and dried at constant weight to obtain oil....

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Abstract

The invention discloses a wall-breaking and surfactant-assisted method used for extracting grease from microalgae containing oil. The wall-breaking and surfactant-assisted method comprises following steps: (1) pH value of a suspension liquid of microalgae containing oil is adjusted to be 7.5 to 14, and is subjected to hydro-thermal treatment; (2) after hydro-thermal treatment, enzymatic hydrolysis is carried out in the presence of enzymes; and (3) after enzymatic hydrolysis, a surfactant is added for reaction, and an organic solvent is added for extraction and separation of grease. Grease extraction rate of the wall-breaking and surfactant-assisted method is high; operation conditions are mild; nutritional ingredients of microalgae can be maintained effectively; using amount of the organic solvent is small; the wall-breaking and surfactant-assisted method is friendly to the environment, is low in energy consumption, and is suitable for nannochloropsis oculata, chlorella, diatom, scenedesmus, and monoraphidium komarkova-legnerova; and application range is wide.

Description

technical field [0001] The invention relates to the technical field of microalgae bioengineering, in particular to a method for breaking the wall of oil-containing microalgae and extracting oil with the aid of a surfactant. Background technique [0002] Microalgae refer to tiny algae groups whose shape can only be distinguished under a microscope, and are the most important primary productivity in the biosphere. Algae has the advantages of large biomass, short production cycle, various types, high oil content, and strong environmental adaptability. It is a good raw material for biodiesel. In addition, microalgae also contains a variety of vitamins, carotene, protein, fatty acids and other components, which are the source of medicinal active substances. [0003] However, microalgae oils are wrapped in cells, and most of the bioactive substances of microalgae such as proteins, carotenoids, and microalgae polysaccharides are present in biological cells. Because microalgae cel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 吴冲冲荣峻峰林伟国侯研博
Owner CHINA PETROLEUM & CHEM CORP
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