Obtaining and application of oil-producing monoraphidium LB59

A technology of monopods and green algae, which is applied in the field of screening and cultivation of oleaginous microalgae, can solve problems such as the shortage of good algae strains, and achieve the effects of reducing costs and preventing damage

Active Publication Date: 2014-01-29
INST OF AQUATIC LIFE ACAD SINICA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem of insufficient fine algae strains used to produce microalgae oil in the prior art, and to provide a kind of Obtaining and its application, that is, collecting water samples in the wild waters of Inner Mongolia, separating and purifying a kind of oil-producing microalgae with stress resistance and easy open culture, and establishing methods for cultivating and collecting

Method used

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  • Obtaining and application of oil-producing monoraphidium LB59
  • Obtaining and application of oil-producing monoraphidium LB59
  • Obtaining and application of oil-producing monoraphidium LB59

Examples

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example 1

[0036] Example 1. Semi-continuous cultivation in a circular photoreactor

[0037] Prepare the culture medium according to the medium formula described above, at 0.1 m 2 Inoculate and culture in a circular photoreactor with a culture medium volume of 20 L. Initial OD 680 The value is 0.05, the temperature is controlled at 30-35°C, and the light intensity is 200 μE / m 2 s continuous light, pass CO2 into the culture medium for 1 hour every day 2 , and sample OD every day 680 value, OD 680 When the value reaches 6.0, collect 1 / 2 of the algae liquid and supplement 1 / 2 of fresh medium for semi-continuous culture, and then collect the algae liquid every 5 days to feed the same volume of medium. In the collection container, the pH of Monostipella LB59 was adjusted to 6 with dilute hydrochloric acid, chitosan was added to 5.0 mg / L, and the flocculation and sedimentation efficiency reached over 90% in 30 minutes; the supernatant was removed from the flocculated algae liquid to obtai...

example 2

[0039] Example 2. Outdoor track pool cultivation

[0040] According to the medium formula of the algae strain, the local groundwater in Inner Mongolia was used to prepare the culture medium. 2 Runway pools for open culture (see Figure 4 ), the volume of culture medium was 750 L. The flow rate of the runway pool is controlled by a wheel-shaped agitator to 0.04 m / s; the water temperature is about 30°C during the day and about 24°C at night; the light intensity varies with the weather, and the average light intensity during the day is 600 μE / m2 s, in a dark state at night; sample and measure OD every day 680 value, when cultured to OD 680 When the value reaches 2.0, start to collect 1 / 5 of the volume of algae cell liquid, then add 1 / 5 of the fresh culture liquid for semi-continuous culture, and then collect feed once every 3 days. The collected algae liquid is observed under a microscope and maintains more than 99% pure species, with little contamination by bacteria and other...

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Abstract

The invention discloses an obtaining and an application of an oil-producing monoraphidium LB59, and relates to a screening and culturing technology of an oil-producing micro-alga. Through subjecting alga seeds separated from a field environment to 18SrDNA sequence analysis and morphological identification, the alga is determined to be a member of Monoraphidium, and the sequence is SEQ ID No.1. The oil-producing monoraphidium LB59 disclosed by the invention can tolerate high-concentrate sodium bicarbonate, is suitable for open type culture, is rich in grease, the grease content can reach 30.2% of dry cell weight under the condition that a growth and collection rate of 23.6g/m<2>d is held, and the fatty acid contains more than 20% of C-18 3-olefine acid and C-18 4-olefine acid.

Description

technical field [0001] The invention relates to the screening and cultivation technology of oleaginous microalgae, and in particular to the acquisition and application of a single needle algae oleaginous LB59. Specifically, the present invention collects water samples from the field, and obtains an oleaginous algal strain that can be used as a raw material for oil production through separation and purification. Background technique [0002] There are more than 40,000 species of algae identified on Earth, some of which are rich in oil. The oil extracted from oil algae can be converted into biodiesel through reaction with methanol, which is considered the most likely to replace the depleted petroleum source of diesel. The 16-carbon and 18-carbon fatty acids it contains are most beneficial for biodiesel production. Algal oils also contain unsaturated fatty acids that can be added to animal feed to promote growth. Screening and obtaining algal species that are easy to cultiva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C11B1/10A23K1/16C12R1/89
Inventor 孔任秋徐旭东席玮芳高宏方仙桃
Owner INST OF AQUATIC LIFE ACAD SINICA
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