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Method for improving grease produced by microalgae

A technology for microalgae and oil, applied in the fields of biotechnology and bioenergy, can solve the problems of cultivating algae growth and reproduction, failure of cultivation, unfavorable cultivation, etc., to increase the oil content of microalgae, avoid bacterial contamination, and improve carbon sequestration efficiency. and oil content

Active Publication Date: 2020-05-05
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the open culture of microalgae is easily polluted by harmful organisms such as filamentous fungi, rotifers, and protozoa. When the number of these harmful organisms reaches a certain density, it will affect the growth and reproduction of the cultured algae. The light algae liquid is not conducive to further expansion of the culture, and the heavy one will lead to the failure of the culture

Method used

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  • Method for improving grease produced by microalgae
  • Method for improving grease produced by microalgae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) In a 20L photobioreactor, add Pseudochlorella kelvinia FSH-Y3 seed solution and microalgae medium, the amount of seed solution added is 800mL, the pH value of the microalgae medium is adjusted to 10, the amount added 8L, the culture temperature is 25°C, the continuous light intensity is 13000Lux, and the CO in the flue gas 2 The content of NO is 5v%, and the content of NO is 50×10 -6 (v / v).

[0035] (2) After 4 days of cultivation, insert 800mL of monospina SHJ-02 seed solution, adjust the pH of the microalgae culture system to 8, culture temperature at 25°C, and introduce CO2 in the flue gas. 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v); The continuous light is changed to light-dark alternating reaction, the light intensity is up to 3000Lux, the light-dark cycle is 2s, and the light-dark time ratio is 1:1.

[0036] (3) After 7 days of culture, enter the stable growth period, end the culture, harvest the microalgae cells by centrifugation...

Embodiment 2

[0039] (1) In a 20L photobioreactor, add Pseudochlorella kelvinia FSH-Y3 seed solution and microalgae medium, the amount of seed solution added is 800mL, the pH value of the microalgae medium is adjusted to 12, the amount added 8L, the culture temperature is 35°C, the continuous light intensity is 10000Lux, and the CO in the flue gas 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v).

[0040] (2) After culturing for 4 days, insert 800ml of Monostipella SHJ-02 seed solution, adjust the pH of the microalgae culture system to 9, culture temperature at 25°C, and introduce CO2 in the flue gas. 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), the continuous light is changed to light-dark alternating reaction, the light intensity reaches 2000Lux; the light-dark cycle is 3s, and the light-dark time ratio is 1:1.

[0041] (3) After cultivating for 7 days, it enters a stable period, ends the culturing, harvests the microalgae cells by centrifugati...

Embodiment 3

[0044] (1) In a 20L photobioreactor, add Pseudochlorella kelvinia FSH-Y3 seed solution and microalgae medium, the amount of seed solution added is 800mL, the pH value of microalgae medium is adjusted to 11, the amount added 8L, the culture temperature is 30°C, the continuous light intensity is 15000Lux, and the CO in the flue gas 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v).

[0045] (2) After culturing for 4 days, insert 800ml of Monostipella SHJ-02 seed liquid, adjust the pH of the microalgae culture system to 10, culture temperature at 30°C, and pass CO2 in the flue gas. 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), the continuous light is changed to light-dark alternating reaction, the light intensity reaches 4000Lux; the light-dark cycle is 4s, and the light-dark time ratio is 1:1.

[0046] (3) After cultivating for 7 days, it enters a stable period, ends the culturing, harvests the microalgae cells by centrifugation, and me...

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Abstract

The invention relates to a method for improving grease produced by microalgae. The method comprises the following steps of: firstly, adding a microalgae culture medium and a Parachlorella kessleri FSH-Y3 or / and scenedesmus obliquus FSH-Y2 seed solution into a photobioreactor, regulating pH to be 10-12, and introducing gases of which the CO2 content is 1-5 v% to culture for certain time, wherein continuous illumination intensity is 8000-20000 Lux; and then, inoculating a MonorapHidium sp SHJ-02 or / and Desmodesmus sp. HCS-02 seed solution, introducing gases of which the CO2 content is 5-45 v%,controlling a pH value to be 8-10, lowering the illumination intensity to 1000-5000 Lux, changing into light and dark alternation reaction, culturing to a stable period, and harvesting microalgae cells, wherein a light and dark period is 0.5-5s, and a light and dark time ratio is 1:1-5. According to the method, the tolerance and the solubleness of a microalgae culture system for high-concentration CO2 can be improved, and biomass and grease content are improved.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for improving oil production by microalgae. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a p...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/64Y02P20/59
Inventor 师文静樊亚超张霖廖莎李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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