Method for producing microalgae grease by using flue gas

A technology for microalgae oil and flue gas, which is applied in the fields of biotechnology and bioenergy, can solve problems such as unfavorable algae absorption and utilization, no oil content involved, unfavorable growth of microalgae, etc. Efficiency, the effect of promoting rapid growth

Active Publication Date: 2019-04-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
[0005] But in practical application, when the environment CO 2 When the volume fraction is greater than 5v%, the growth of most microalgae will be inhibited, and the carbon fixation efficiency will be low
At the same time, general microalgae are suitable for growth under neutral conditions, and are no

Method used

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  • Method for producing microalgae grease by using flue gas
  • Method for producing microalgae grease by using flue gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) In a 10L photobioreactor, add Pseudochlorella kescheria FSH-Y3 seed solution and microalgae medium prepared in Example 1, the amount of seed solution added is 400mL, and the pH value of the microalgae medium is adjusted 10, the addition amount is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and CO 2 The content of NO is 5v%, and the content of NO is 50×10 -6 (v / v), SO 2 Content is 60×10 -6 (v / v).

[0034] (2) After 4 days of cultivation, insert 400 mL of the Scenedesmus MH-04 seed liquid prepared in Example 1 and 400 mL of the Chlorella SF-B1 seed liquid, adjust the pH of the microalgae culture system to 8, and cultivate continuously under light. 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0035] (3) After 7 days of culture, enter the stable growth period, end the culture, harvest the mic...

Embodiment 2

[0037] (1) In a 10L photobioreactor, add Pseudochlorella kescheria FSH-Y3 seed solution and microalgae medium prepared in Example 1, the amount of seed solution added is 800mL, and the pH value of the microalgae medium is adjusted 12, the addition amount is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, CO 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v), SO 2 The content is 100×10 -6 (v / v).

[0038] (2) After 2 days of cultivation, insert 560 mL of the Scenedesmus MH-04 seed liquid prepared in Example 1 and 560 mL of the Chlorella SF-B1 seed liquid, adjust the pH of the microalgae culture system to 9, and cultivate in continuous light. 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0039] (3) After cultivating for 8 days, it enters the stationary phase, ends the culturing, harvests the m...

Embodiment 3

[0041] (1) In a 10L photobioreactor, add the pseudo-Kjeldahl FSH-Y3 seed solution prepared in Example 1 and the microalgae medium, the amount of the seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 12. The amount added is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v), SO 2 The content is 100×10 -6 (v / v).

[0042] (2) After 2 days of cultivation, insert 560 mL of the seed liquid of Monostipella SS-B1 prepared in Example 1 and 560 mL of the seed liquid of Chlorella SF-B1, adjust the pH of the microalgae culture system to 9, and cultivate under continuous light. The intensity is 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).

[0043] (3) After cultivating for 8 days,...

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Abstract

The invention relates to a method for producing microalgae grease by using flue gas. The method for the producing microalgae grease by using the flue gas comprises the following steps that firstly, amicroalgae culture medium and parachlorella kessleri FSH-Y3 or/and a scenedesmus obliquus FSH-Y2 seed solution are added into a photobioreactor, the pH value is adjusted to 10-12, the flue gas with CO2 content of 1v-5v% is introduced, and culture is carried out for a certain time; and then the pH value is adjusted to 8-10, a chlorella SF-B1 seed solution is introduced, at least one of a scenedesmus MH-04 seed solution a monoraphidium SS-B1 seed solution is introduced, mixed culture is carried out, the flue gas with CO2 content of 5v%-45v% is introduced, and the mixture is cultured to a stableperiod under the condition of continuous illumination, so that microalgae cells are obtained. The method for producing the microalgae grease by using the flue gas improves the tolerance and solubilityof a microalgae culture system to high-concentration CO2, improves the carbon fixation efficiency, obviously improves the harvest amount of the microalgae grease, simultaneously improves the tolerance to SOx and NOx in the flue gas, and can purify the flue gas.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for producing microalgae oil by using flue gas. Background technique [0002] As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a potential resource for the production of pharmaceuticals, fine chemicals and new fuels. The fatty acids obtained from microalgae can be converted into fatty acid methyl ester, that is, biodiesel. Therefore, ...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/64C12P7/6463
Inventor 师文静王鹏翔李晓姝孙启梅张霖樊亚超
Owner CHINA PETROLEUM & CHEM CORP
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