Method for producing microalgae grease by using flue gas
A technology for microalgae oil and flue gas, which is applied in the fields of biotechnology and bioenergy, can solve problems such as unfavorable algae absorption and utilization, no oil content involved, unfavorable growth of microalgae, etc. Efficiency, the effect of promoting rapid growth
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Embodiment 1
[0033] (1) In a 10L photobioreactor, add Pseudochlorella kescheria FSH-Y3 seed solution and microalgae medium prepared in Example 1, the amount of seed solution added is 400mL, and the pH value of the microalgae medium is adjusted 10, the addition amount is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and CO 2 The content of NO is 5v%, and the content of NO is 50×10 -6 (v / v), SO 2 Content is 60×10 -6 (v / v).
[0034] (2) After 4 days of cultivation, insert 400 mL of the Scenedesmus MH-04 seed liquid prepared in Example 1 and 400 mL of the Chlorella SF-B1 seed liquid, adjust the pH of the microalgae culture system to 8, and cultivate continuously under light. 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).
[0035] (3) After 7 days of culture, enter the stable growth period, end the culture, harvest the mic...
Embodiment 2
[0037] (1) In a 10L photobioreactor, add Pseudochlorella kescheria FSH-Y3 seed solution and microalgae medium prepared in Example 1, the amount of seed solution added is 800mL, and the pH value of the microalgae medium is adjusted 12, the addition amount is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, CO 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v), SO 2 The content is 100×10 -6 (v / v).
[0038] (2) After 2 days of cultivation, insert 560 mL of the Scenedesmus MH-04 seed liquid prepared in Example 1 and 560 mL of the Chlorella SF-B1 seed liquid, adjust the pH of the microalgae culture system to 9, and cultivate in continuous light. 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).
[0039] (3) After cultivating for 8 days, it enters the stationary phase, ends the culturing, harvests the m...
Embodiment 3
[0041] (1) In a 10L photobioreactor, add the pseudo-Kjeldahl FSH-Y3 seed solution prepared in Example 1 and the microalgae medium, the amount of the seed solution added is 800mL, and the pH value of the microalgae medium is adjusted to 12. The amount added is 8L, the light intensity for cultivation is 5000Lux, the light cycle is 24h, the light-dark time ratio is 14:10, and the CO in the flue gas is introduced 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (v / v), SO 2 The content is 100×10 -6 (v / v).
[0042] (2) After 2 days of cultivation, insert 560 mL of the seed liquid of Monostipella SS-B1 prepared in Example 1 and 560 mL of the seed liquid of Chlorella SF-B1, adjust the pH of the microalgae culture system to 9, and cultivate under continuous light. The intensity is 5000Lux; through the CO in the flue gas 2 The content of NO is 40v%, and the content of NO is 800×10 -6 (v / v), SO 2 The content is 600×10 -6 (v / v).
[0043] (3) After cultivating for 8 days,...
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