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Oil-producing monoraphidium sp. as well as culture and application thereof

A single needle algae and culture medium technology, applied in the fields of biotechnology and bioenergy, can solve the problems of slow growth, poor low temperature resistance, death, etc., and achieve the effects of strong low temperature resistance, high oil content, and fast growth rate

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, some of the microalgae isolated and screened out above cannot utilize CO 2 Carry out autotrophic growth, some have poor low temperature resistance, under low temperature conditions (below 15°C), the growth rate is slow, and some even die
In the cold weather in winter, it is necessary to heat and cultivate, and the energy consumption is high

Method used

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  • Oil-producing monoraphidium sp. as well as culture and application thereof
  • Oil-producing monoraphidium sp. as well as culture and application thereof
  • Oil-producing monoraphidium sp. as well as culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Separation and screening to obtain Monostipella SHJ-02

[0027] (1) Enrichment culture: Water samples were collected from the Songhua River in Heilongjiang in October 2011, filtered through gauze to remove large solids; 80mL of filtered water samples were inoculated into 200mL of BG11 medium for enrichment culture, and the cultured The light intensity was 8000Lux, the temperature was 25°C, the light-dark cycle was 24h, and the light-dark time ratio was 14:10. After half a month of cultivation, the culture medium was obviously green, and a large number of algae were observed under the microscope. The formulation of the BG11 medium is shown in Table 1 and Table 2.

[0028] Table 1 BG11 medium

[0029]

[0030] *Table 2 Composition of A5+Co solution in Table 1

[0031]

[0032] (2) Obtaining pure algae strains: Dilute the enriched water sample to 10 -5 times, under sterile conditions, spread it on the BG11 solid plate and cultivate it. The light intensit...

Embodiment 2

[0034] Example 2 Identification of algae strains

[0035] The DNA of the obtained SHJ-02 algal cells was extracted by CTAB method, and the 18S rDNA gene was cloned, and the three positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Monoraphidium sp. It has the greatest similarity, its BLASTn value is 2619, and its Max index value is 99%, so it can be determined that SHJ-02 is Monospirulina ( Monoraphidium sp. ).

Embodiment 3

[0036] Example 3 Cultivation and Application of Monostipella SHJ-02

[0037](1) Prepare BG11 freshwater microalgae culture medium according to Table 1 and Table 2, pack in 250mL Erlenmeyer flasks, the volume of each bottle is 50mL, seal with air-permeable film, wrap with kraft paper for sterilization, and sterilize at a temperature of 121°C , Sterilize at a pressure of 0.1MPa for 20-30min.

[0038] (2) Select a single algae colony SHJ-02 from the solid plate and inoculate it into the above medium, and carry out in a constant temperature light reaction shaker at 5-33°C, the light intensity is 8000Lux, the light-dark reaction cycle is 24h, and the light-dark time The ratio was 14:10, the shaker speed was 100rpm, and cultured to the exponential growth phase.

[0039] (3) Transfer the algae liquid cultured in step (2) to a tubular photoreactor filled with BG11 medium, and culture in aeration with an aeration rate of 1.0vvm, CO 2 The content is 2% (v / v), the temperature is 5-30°C...

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Abstract

The invention relates to monoraphidium sp. SHJ-02, with the classification name of Monoraphidium sp. preserved in CGMCC (China General Microbiological Culture Collection Center) of CCCCM (China Committee for Culture Collection of Microorganisms) on April 24, 2015, with the preservation number being CGMCC N0.10763. The strain can grow efficiently under the autotrophy condition to obtain oil-rich biomass; besides, the strain has high environment adaptability, can still grow more quickly at the low temperature, has high oil content, has the cell total lipid content being 40% of the dry cell weight of cells or above and can be applied to production of biodiesel.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a single needle algae producing oil and its cultivation application. Background technique [0002] With the rapid development of society, fossil energy is increasingly scarce, and environmental pollution is becoming more and more serious. Energy issues have become the key to restricting the development of the entire human society. Finding a clean, economical, efficient, and renewable new energy has become an urgent problem to be solved. , the world is gradually moving towards the "post-oil era". [0003] Biomass is a sustainable, renewable, low-cost new energy that can replace fossil fuels. Microalgae, as an aquatic biomass resource, has the advantages of high photosynthetic efficiency, fast growth rate, high biomass yield, and strong environmental adaptability. It has comprehensive utilization value and can be used to produce biomass energy, including bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/10
Inventor 孙启梅师文静廖莎王鹏翔王领民王崇辉高大成
Owner CHINA PETROLEUM & CHEM CORP
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