Method for inducing fresh water chlorella to fast accumulate grease through plant hormone jasmonic acid

Abstract

The invention provides a method for inducing fresh water chlorella to fast accumulate grease through plant hormone jasmonic acid. The method is characterized by comprising the following steps of: (1) preparation of chlorella solution preparation: under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio is 12h / 12h, culturing fresh water chlorella to logarithmic phase to obtain chlorella solution for conducting hormone induction, wherein the fresh water chlorella adopts NO.9 fresh water chlorella or NO.31 fresh water chlorella which both come from Freshwater Algae Culture Collection at the Institute of Hydrobiology, FACHB-collection; (2) acquisition of high-grease-content chlorella: adding jasmonic acid into 200ml of chlorella solution obtained in step (1) until the final concentration is 0.49-0.51mg / l; and (3) acquisition of high-grease-content chlorella: culturing the chlorella solution subjected to hormone induction in step (2) for 15 days under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio of 12h / 12h, and centrifugally collecting the frond with high grease content. The method is simple and easy, low in cost, and the grease content of the target product is high, and can be used as a raw material of industrialized biological produced oil.

Description

technical field

[0001] The invention provides a method for the plant hormone jasmonic acid to induce freshwater chlorella to rapidly accumulate oil, and belongs to the field of biotechnology. Background technique

[0002] Chlorella (Chlorella) is a spherical single-celled freshwater algae, which is an important genus in the Chlorella family of green algae. It has a wide ecological distribution and is distributed in both seawater and freshwater. Currently, it includes about 10 species. Chlorella cells are rich in protein, essential amino acids, polysaccharides, lipids, chlorophyll, carotene and vitamins, etc., and are widely used in health food, aquaculture, cosmetics, medicine and other fields. Due to the advantages of fast reproduction and high oil content, chlorella has become a biodiesel raw material with application and development prospects. At present, mastering certain induction conditions that promote oil accumulation is one of the effective ways to reduce the cost ...

Examples

Embodiment 1

[0013] Step 1. Preparation of algae liquid: Under the conditions of temperature 22°C, light intensity 1800lx, and light-to-dark ratio 12h / 12h, cultivate No. 9 freshwater Chlorella cells from the freshwater algae bank of the Institute of Hydrobiology, Chinese Academy of Sciences to logarithmic growth During the period, the algae liquid that will be induced by hormones is obtained;

[0014] Step 2. Hormone induction: Add jasmonic acid (J2500-100MG) produced by SIGMA Company of the United States to 200ml of algae liquid obtained in step 1 until the final concentration is 0.49mg / l;

[0015] Step 3. Obtaining algae with high oil content: After culturing the algae fluid induced by the hormone in step 2 for 15 days at a temperature of 22°C, a light intensity of 1800lx, and a light-to-dark ratio of 12h / 12h, the algae with high oil content were collected by centrifugation body.

[0016] Test data: the fat content of the algal body finally obtained in Step 3 of the present invention is...

Embodiment 2

[0018] Step 1. Preparation of algae liquid: Under the conditions of temperature 22°C, light intensity 1800lx, and light-dark ratio 12h / 12h, cultivate No. 31 freshwater Chlorella cells from the freshwater algae stock of the Institute of Hydrobiology, Chinese Academy of Sciences to logarithmic growth During the period, the algae liquid that will be induced by hormones is obtained;

[0019] Step 2. Hormone induction: Add jasmonic acid (J2500-100MG) produced by SIGMA Company of the United States to 200ml of algae liquid obtained in step 1 until the final concentration is 0.49mg / l;

[0020] Step 3. Obtaining algae with high oil content: After culturing the algae fluid induced by the hormone in step 2 for 15 days at a temperature of 22°C, a light intensity of 1800lx, and a light-to-dark ratio of 12h / 12h, the algae with high oil content were collected by centrifugation body.

[0021] Test data: the fat content of the algal body finally obtained in step 3 of the present invention is ...

Embodiment 3

[0023] Step 1. Preparation of algae liquid: Under the conditions of temperature 23°C, light intensity 1900lx, and light-to-dark ratio 12h / 12h, cultivate No. 9 freshwater Chlorella cells from the freshwater algae stock of the Institute of Hydrobiology, Chinese Academy of Sciences to logarithmic growth During the period, the algae liquid that will be induced by hormones is obtained;

[0024] Step 2. Hormone induction: Add jasmonic acid (J2500-100MG) produced by SIGMA Company of the United States to 200ml of algae liquid obtained in step 1 until the final concentration is 0.5mg / l;

[0025] Step 3. Obtaining algae with high oil content: After culturing the algae fluid induced by the hormone in step 2 for 15 days at a temperature of 23°C, a light intensity of 1900lx, and a light-to-dark ratio of 12h / 12h, the algae with high oil content were collected by centrifugation body.

[0026] Test data: the fat content of the algal body finally obtained in step 3 of the present invention is...

technical field

[0001] The invention provides a method for the plant hormone jasmonic acid to induce freshwater chlorella to rapidly accumulate oil, and belongs to the field of biotechnology. Background technique

[0002] Chlorella (Chlorella) is a spherical single-celled freshwater algae, which is an important genus in the Chlorella family of green algae. It has a wide ecological distribution and is distributed in both seawater and freshwater. Currently, it includes about 10 species. Chlorella cells are rich in protein, essential amino acids, polysaccharides, lipids, chlorophyll, carotene and vitamins, etc., and are widely used in health food, aquaculture, cosmetics, medicine and other fields. Due to the advantages of fast reproduction and high oil content, chlorella has become a biodiesel raw material with application and development prospects. At present, mastering certain induction conditions that promote oil accumulation is one of the effective ways to reduce the cost ...

Examples

Embodiment 1

[0013] Step 1. Preparation of algae liquid: Under the conditions of temperature 22°C, light intensity 1800lx, and light-to-dark ratio 12h / 12h, cultivate No. 9 freshwater Chlorella cells from the freshwater algae bank of the Institute of Hydrobiology, Chinese Academy of Sciences to logarithmic growth During the period, the algae liquid that will be induced by hormones is obtained;

[0014] Step 2. Hormone induction: Add jasmonic acid (J2500-100MG) produced by SIGMA Company of the United States to 200ml of algae liquid obtained in step 1 until the final concentration is 0.49mg / l;

[0015] Step 3. Obtaining algae with high oil content: After culturing the algae fluid induced by the hormone in step 2 for 15 days at a temperature of 22°C, a light intensity of 1800lx, and a light-to-dark ratio of 12h / 12h, the algae with high oil content were collected by centrifugation body.

[0016] Test data: the fat content of the algal body finally obtained in Step 3 of the present invention is...

Embodiment 2

[0018] Step 1. Preparation of algae liquid: Under the conditions of temperature 22°C, light intensity 1800lx, and light-dark ratio 12h / 12h, cultivate No. 31 freshwater Chlorella cells from the freshwater algae stock of the Institute of Hydrobiology, Chinese Academy of Sciences to logarithmic growth During the period, the algae liquid that will be induced by hormones is obtained;

[0019] Step 2. Hormone induction: Add jasmonic acid (J2500-100MG) produced by SIGMA Company of the United States to 200ml of algae liquid obtained in step 1 until the final concentration is 0.49mg / l;

[0020] Step 3. Obtaining algae with high oil content: After culturing the algae fluid induced by the hormone in step 2 for 15 days at a temperature of 22°C, a light intensity of 1800lx, and a light-to-dark ratio of 12h / 12h, the algae with high oil content were collected by centrifugation body.

[0021] Test data: the fat content of the algal body finally obtained in step 3 of the present invention is ...

Embodiment 3

[0023] Step 1. Preparation of algae liquid: Under the conditions of temperature 23°C, light intensity 1900lx, and light-to-dark ratio 12h / 12h, cultivate No. 9 freshwater Chlorella cells from the freshwater algae stock of the Institute of Hydrobiology, Chinese Academy of Sciences to logarithmic growth During the period, the algae liquid that will be induced by hormones is obtained;

[0024] Step 2. Hormone induction: Add jasmonic acid (J2500-100MG) produced by SIGMA Company of the United States to 200ml of algae liquid obtained in step 1 until the final concentration is 0.5mg / l;

[0025] Step 3. Obtaining algae with high oil content: After culturing the algae fluid induced by the hormone in step 2 for 15 days at a temperature of 23°C, a light intensity of 1900lx, and a light-to-dark ratio of 12h / 12h, the algae with high oil content were collected by centrifugation body.

[0026] Test data: the fat content of the algal body finally obtained in step 3 of the present invention is...