Method based on mixotroph microalgae oil accumulation

A technology of microalgae oil and basal medium is applied in the field of autotrophic induction of microalgae oil accumulation in molasses alcohol waste mash, which can solve the problems of oil accumulation, low biomass yield and high-quality yield, etc. Promotes the effect of increasing, improving the utilization and conversion of sugar

Inactive Publication Date: 2018-09-28
KUNMING UNIV OF SCI & TECH
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  • Description
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AI Technical Summary

Problems solved by technology

However, the biomass yield and high-quality yield of admixture are low, so admixture is generally not suitable for accumulating oil

Method used

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  • Method based on mixotroph microalgae oil accumulation

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Embodiment 1

[0022] A method based on the oil accumulation of concurrently supported microalgae, specifically comprising the following steps:

[0023] (1) BG-11 basal medium with glucose as carbon source and culture of oleaginous microalgae Monoraphidium sp. FXY-10, collect the algae cells after the growth of Monopodella to the late logarithmic phase; the amount of glucose added is 9g / L, and the temperature for culturing Monopodella is 26°C.

[0024] (2) Preparation of induction medium: the waste mash was diluted 3200 times and used as the basic medium of Monostipella, and then MgSO was added to the basic medium 4 ·7H 2 O, the addition amount is 287.8mg / L; adjust the pH value to 6.8-7.1, and prepare it as a culture medium.

[0025] (3) Cultivation of oleaginous microalgae: The culture medium obtained in step (2) was subjected to high-pressure high-temperature sterilization, and then inserted into the monospora oleaginum in step (1), the algae cell concentration was 0.9g / L, and then adde...

Embodiment 2

[0030] A method based on the oil accumulation of concurrently supported microalgae, specifically comprising the following steps:

[0031] (1) BG-11 basal medium with glucose as carbon source and culture of oleaginous microalgae Monoraphidium sp. FXY-10, collect the algae cells after the growth of Monostipella to the late logarithmic phase; the amount of glucose added is 11g / L, and the temperature for cultivating Monotipella is 23°C.

[0032] (2) Preparation of induction medium: Dilute the waste mash 3250 times and use it as the basic medium of Monostipella, and then add MgSO to the basic medium 4 ·7H 2 O, the addition amount is 293mg / L; adjust the pH value to 6.8-7.1, and prepare it as a culture medium.

[0033] (3) Cultivation of oleaginous microalgae: The culture medium obtained in step (2) was subjected to high-pressure high-temperature sterilization, and then inserted into the monospora oleaginum in step (1), the algae cell concentration was 1g / L, and then added with an...

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Abstract

The invention discloses a method based on mixotroph microalgae oil accumulation, and belongs to the technical field of biodiesel. The method comprises the steps that a BG-11 basal culture medium adopting glucose as a carbon source is used for cultivating single algae, and algae cells are collected when the single algae grows to the rear logarithmic growth period; waste fermented liquid is dilutedto be adopted as the basal culture medium for single algae, and then, MgSO4 7H2O is added into the basal culture medium; the PH value is regulated to 6.8-7.1, and the culture medium is prepared; the prepared culture medium is subjected to high-pressure and high-temperature sterilization, oil-producing single algae is inoculated, the algae cell concentration is 0.9-1 g/L, then, absolute ethyl alcohol is added for dissolving a melatonin mother solution, the final concentration of melatonin in the culture medium is 10<-1>-10<-4> mol/L, and the algae solution is placed into strong light to be cultured; microalgae cultured to the stable stage is enriched, oil is extracted, and biodiesel is prepared. The method is simple and easy to implement, the production cost can be greatly lowered, the oilyield is remarkably increased, and therefore the biodiesel production efficiency of microalgae is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biodiesel, and in particular relates to a method for cultivating microalgae by using a concurrently cultured BG-11 medium, and then using molasses alcohol waste mash to self-support to induce oil accumulation in the microalgae. Background technique [0002] Biodiesel, commonly known as sunshine fuel, is a long-chain fatty acid monoalkyl ester, which is prepared by transesterification with short-chain alcohols (such as methanol and ethanol) from vegetable oils, animal oils, various waste oils, and microbial oils. An oxygen-containing regenerative clean fuel that can replace petrochemical diesel is obtained. Compared with conventional diesel, it has the characteristics of environmental protection, renewable, easy to degrade, low pollutant emission after combustion, and low greenhouse gas emission. [0003] In recent years, microalgae have attracted much attention as a raw material for biodiesel production....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/38C12P7/64C12R1/89
CPCC12N1/12C12N1/38C12P7/6463Y02E50/10
Inventor 余旭亚宋雪婷董训赞
Owner KUNMING UNIV OF SCI & TECH
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