Method for high production of microorganism grease by stuffing batch culture

A microbial oil and feeding technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of reducing production costs, simple and easy feeding methods, and increasing oil content and oil output

Inactive Publication Date: 2008-04-02
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to technical and economic reasons, there were few reports of large-scale production of single-cell oils in the past, but with the development of industrial biotechnology, new progress has been made in microbial oil fermentation from raw materials to processes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] (1) Strain: Rhodotorula Glutinis (purchased from Institute of Microbiology, Chinese Academy of Sciences)

[0014] (2) culture medium

[0015] a. Solid medium

[0016] Yeast powder: 1%; peptone: 2%; glucose: 2%; agar: 2%; pH=6.

[0017] b. Seed medium

[0018] Glucose: 1.5%; (NH 4 ) 2 SO 4 : 0.2%; yeast powder: 0.1%; KH 2 PO 4 : 0.7%; Na 2 HPO 4 : 0.2%; MgSO 4 : 0.15%; pH=6.0.

[0019] c. Fermentation medium

[0020] Glucose: 5%; (NH 4 ) 2 SO 4 : 0.25%; yeast powder: 0.1%; KH 2 PO 4 : 0.7%; Na 2 HPO 4 : 0.2%; MgSO 4 : 0.2%; CaCl 2 : 0.01%; FeCl 3 : 0.007%; pH=6.0.

[0021] (3) Training method

[0022] The strains preserved in glycerol tubes were transferred to solid slant medium and activated at 30°C for 12 hours. Seed culture adopts aerobic culture, using a 500ml Erlenmeyer flask with a liquid volume of 50ml. The culture temperature is 30° C., and the shaker rotates at 180 rpm. The seeds were cultured for 28 hours with 5% inoculum amount insert...

example 2

[0026] (1) Strain: Rhodotorula Glutinis (purchased from Institute of Microbiology, Chinese Academy of Sciences)

[0027] (2) culture medium

[0028] (A) Solid medium

[0029] Same as instance 1

[0030] (B) Seed medium

[0031] Glucose: 1.5%; KNO 3 : 0.3%; yeast powder: 0.1%; KH 2 PO 4 : 0.7%; Na 2 HPO 4 : 0.2%; MgSO 4 : 0.15%; pH=6.0.

[0032] (C) Fermentation medium

[0033] Glucose: 5%; KNO 3 : 0.4%; yeast powder: 0.1%; KH 2 PO 4 : 0.7%; Na 2 HPO 4 : 0.2%; MgSO 4 : 0.2%; CaCl 2 : 0.01%; FeCl 3 : 0.007%; pH=6.0.

[0034] (3) Training method

[0035] The strains preserved in glycerol tubes were transferred to solid slant medium and activated at 30°C for 12 hours. Seed culture adopts aerobic culture, using a 500ml Erlenmeyer flask with a liquid volume of 50ml. The culture temperature is 30° C., and the shaker rotates at 180 rpm. The seeds were cultured for 28 hours with 5% inoculum amount inserted into the fermentation medium, and the fermentation culture...

example 3

[0039] (1) Strain: Rhodotorula Glutinis (purchased from Institute of Microbiology, Chinese Academy of Sciences)

[0040] (2) culture medium: with example 1

[0041] (A) Solid medium

[0042] Same as instance 1

[0043] (B) Seed medium

[0044] Same as example 2

[0045] (C) Fermentation medium

[0046] Same as example 2

[0047] (3) Culture method: Seed culture is the same as Example 1, and the fermentation culture adopts a 5L fermenter with a liquid volume of 3L, an air flow rate of 1.0vvm, and a stirring speed of 520rpm. After the fermentation was carried out for 30 hours, 60 g of glucose was added (calculated as 20 g / l), and 4.35 g of potassium nitrate was added simultaneously (calculated as C / N=40). Batch culture without feeding was used as control.

[0048] (4) Fermentation results

[0049]After 66 hours of fermentation, the biomass of the control bacteria reached 17.2g / l, the oil content was 40%, and the oil yield was 6.9g / l. The biomass of the fed-batch culture...

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Abstract

A fed-batch method for cultivating microorganism oil with high yield is provided, belonging to the biological chemical technical field. A carbon source is added to a fermentation substrate in the logarithm proliferation of the bacterium, and fed-batch of nitrogen source is carried out with the C / N ration equaling to 40. The invention is characterized in that both the content and the yield of the microorganism oil can be increased significantly. The fed-batch method is easy with convenient operation.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for cultivating high-yield microbial oil in fed batch mode. Background technique [0002] At present, the global energy shortage and environmental degradation are becoming more and more serious. From the perspective of environmental protection and resource development, people have to actively develop renewable new energy to replace fossil fuels. Biodiesel is a renewable and clean energy with great development potential. energy. At present, the research on biodiesel preparation at home and abroad mainly focuses on vegetable oil or waste catering oil, but the cost of using vegetable oil as raw material is high, and its cost accounts for 70% to 85% of the total production cost. The difficulty of collecting and transporting raw materials has seriously restricted the industrialization process of biodiesel, so finding a cheap raw material has become t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 刘宏娟张建安李建墨玉欣程可可周玉杰
Owner TSINGHUA UNIV
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