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Desmodesmus sp. rich in oil, and culture and application thereof

A technology of Scenedesmus and oil, applied in the field of microbial engineering, can solve the problem of long cultivation time, and achieve the effects of rapid sedimentation, easy collection and short growth period

Active Publication Date: 2017-03-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And it needs to be cultured for 17 days, and the culture time is longer

Method used

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  • Desmodesmus sp. rich in oil, and culture and application thereof
  • Desmodesmus sp. rich in oil, and culture and application thereof
  • Desmodesmus sp. rich in oil, and culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Separation and domestication screening to obtain Scenedesmus MH-04

[0023] (1) Obtaining the starting algae strain: In October 2011, 150mL of water samples were retrieved from Daqingming Lake in Heilongjiang, filtered with gauze to remove large impurities, and 50ml of the filtered water samples were inoculated into 200mL of BG11 medium For enrichment culture, the light intensity of the culture is 5000Lux, the temperature is 25°C, the light-dark cycle is 24h, and the light-dark time ratio is 14:10. After about half a month of culture, the culture medium turns green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000Lux and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cultivation, the cultivation temperature i...

Embodiment 2

[0028] Example 2 Identification of algae strains

[0029] The DNA of the obtained MH-04 algae cells was extracted using the CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Desmodesmus sp. There is the greatest similarity, its BLASTn value is 3145, the Max index value is 99%, it can be determined that MH-04 is Scenedesmus ( Desmodesmus sp. ).

Embodiment 3

[0030] Example 3 Application of Scenedesmus MH-04

[0031] The algae liquid of MH-04 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formula of BG11 medium is shown in Table 1 and Table 2. The cultivation was carried out in a laboratory-made column reactor (with an inner diameter of 40mm and a height of 500mm), the OD of the culture medium after inoculation 690 is 0.2. CO was introduced from the bottom of the reactor 2 Flue gas with a content of 30v%, of which SO 2 The content is 400×10 -6 (v / v). During the cultivation process, the light intensity was 5000Lux, the cultivation temperature was 28°C, the pH value was controlled between 7-9, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 8 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder was measured ...

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Abstract

The invention discloses Desmodesmus sp. rich in oil, and culture and application thereof. The Desmodesmus sp. is MH-04 preserved in China General Microbiological Culture Collection Center on April 24, 2015, with an accession number of CGMCC No. 10764. The Desmodesmus sp. can tolerate high-concentration CO2 and SO2, is capable of realizing photoautotrophic growth in virtue of exhaust gas or flue gas containing CO2 and SO2 to acquire biomass and has a high carbon sequestration rate; and the obtained biomass is rich in oil and can be used for production of biodiesel.

Description

technical field [0001] The invention belongs to the field of microbial engineering, and in particular relates to an oil-rich Scenedesmus and its cultivation application. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is a pote...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCY02E50/10
Inventor 师文静孙启梅王鹏翔廖莎李晓姝张霖
Owner CHINA PETROLEUM & CHEM CORP
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