Preparation method and application of skin stem cell activity hormone

A technology of skin stem cells and mesenchymal stem cells, applied in the biological field, can solve problems such as the degradation of bioactive components, achieve the effects of promoting proliferation, promoting iron transportation, and improving skin aging

Active Publication Date: 2017-11-28
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems in the prior art that the crushing of skin stem cells leads to the degradation of bioactive components, the present invention provides a preparation method and application of skin stem cell active ingredients

Method used

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  • Preparation method and application of skin stem cell activity hormone
  • Preparation method and application of skin stem cell activity hormone
  • Preparation method and application of skin stem cell activity hormone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 A kind of preparation method of skin stem cell activating element

[0029] S1. Isolation of placental mesenchymal stem cells: take fresh placental tissue from clinically normal cesarean section, separate the middle inner layer tissue of the placenta under sterile conditions, cut it into a size of 1cm×1cm×1cm, soak it in 400u / ml penicillin for 10min, Rinse 3 times with D-Hank's solution at 4°C, digest with trypsin 0.1g / mL and collagenase 0.05g / mL at 37°C for 30min, filter through a 200-mesh filter to collect tissue cells, red blood cell lysate in water bath at 4°C for 3min to remove red blood cells instant;

[0030] S2. Inoculation, culture and induced differentiation of placental mesenchymal stem cells: inoculate the placental mesenchymal stem cells obtained in step S1 in skin stem cell culture medium, at 37°C, 5% CO 2 1. Incubate in the incubator for 15 hours, discard the culture medium, and re-add fresh skin stem cell culture medium, and when the skin st...

Embodiment 2

[0033] Embodiment 2 A kind of preparation method of skin stem cell activating element

[0034] S1. Isolation of placental mesenchymal stem cells: take fresh placental tissue from clinically normal cesarean section, separate the middle inner layer tissue of the placenta under sterile conditions, cut it into a size of 1cm×1cm×1cm, soak it in 500u / ml penicillin for 20min, Rinse 3 times with D-Hank's solution at 4°C, digest with trypsin 0.15g / mL and collagenase 0.065g / mL at 37°C for 45min, filter through a 200-mesh filter to collect tissue cells, red blood cell lysate in water bath at 4°C for 4min to remove red blood cells instant;

[0035] S2. Inoculation, culture and induced differentiation of placental mesenchymal stem cells: inoculate the placental mesenchymal stem cells obtained in step S1 in skin stem cell culture medium, at 37°C, 5% CO 2 1. Incubate in the incubator for 20 hours, discard the medium, and re-add fresh skin stem cell culture medium, and when the skin stem cel...

Embodiment 3

[0038] Embodiment 3 A kind of preparation method of skin stem cell active element

[0039] S1. Isolation of placental mesenchymal stem cells: take fresh placental tissue from clinically normal cesarean section, separate the middle inner layer tissue of the placenta under sterile conditions, cut it into a size of 1cm×1cm×1cm, soak it in 600u / ml penicillin for 30min, Rinse 3 times with D-Hank's solution at 4°C, digest with trypsin 0.2g / mL and collagenase 0.08g / mL at 37°C for 60min, filter through a 200-mesh filter to collect tissue cells, red blood cell lysate in water bath at 4°C for 5min to remove red blood cells instant;

[0040] S2. Inoculation, culture and induced differentiation of placental mesenchymal stem cells: inoculate the placental mesenchymal stem cells obtained in step S1 in skin stem cell culture medium, at 37°C, 5% CO 2 1. Incubate in the incubator for 25 hours, discard the culture medium, and re-add fresh skin stem cell culture medium, and when the skin stem c...

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Abstract

The invention belongs to the technical field of biology and particularly relates to a preparation method and application of a skin stem cell activity hormone. The preparation method of the skin stem cell activity hormone is obtained by taking placenta mesenchymal stem cells as raw materials, carrying out inoculated culture, carrying out induced differentiation and preparing and collecting the skin stem cell activity hormone. The preparation method of the skin stem cell activity hormone has the technical advantages that trypsin and collagenase are compounded for carrying out enzymolysis on placenta tissue so as to disperse tissue cells; a skin stem cell culture medium is prepared by adding a series of functional components into a basic culture medium; induced differentiation of the placenta mesenchymal stem cells is facilitated to obtain skin stem cells; subsequent multiplication culture of the skin stem cells is facilitated. After the prepared skin stem cell activity hormone is applied to cream cosmetics, the aging of the skin can be obviously improved; the occurrence of adverse effects is reduced; the water content and the oil content of cuticle can be increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method and application of skin stem cell active factor. Background technique [0002] Skin aging is a skin aging phenomenon caused by natural or unnatural factors. Skin aging is closely related to cell aging, especially to the aging of fibroblasts and keratinocytes. Stem cells are undifferentiated cells that have unlimited self-renewal and replication capabilities and can differentiate into specific tissues. Studies have shown that the self-replication and multipotential differentiation of stem cells also depend on a variety of growth factors, cytokines, active peptides, proteins, Substances such as growth hormone and trace elements promote self-amplification and proliferation of differentiated daughter cells. [0003] Chinese patent CN103202794B discloses a preparation method of skin stem cell active factor and its application. One of the main inventions i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/362A61K8/64A61K8/66A61K8/73A61K8/92A61K8/99A61Q19/08C12N5/0775
CPCA61K8/362A61K8/64A61K8/66A61K8/73A61K8/736A61K8/927A61K8/988A61K8/99A61Q19/08C12N5/0668C12N2500/24C12N2500/30C12N2500/32C12N2501/11C12N2501/115C12N2501/13C12N2501/30C12N2501/33
Inventor 车七石刘少辉
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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