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Microalgae culture method and photo-bioreactor system thereof

A technology for photobioreactor and microalgae cultivation, applied in the field of new photobioreactor system, can solve the problems of many reactors, complicated liquid and liquid transportation, no supplementary light system, etc., achieve rapid biomass accumulation, prevent light intensity Insufficient, simple and convenient operation

Active Publication Date: 2010-04-21
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical solution of this invention does not have a supplementary light system, so it is difficult to ensure the rapid growth of microalgae in the first-stage reactor, and there are many reactors, the liquid-liquid transportation between the reactors is cumbersome, and the energy consumption is large

Method used

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  • Microalgae culture method and photo-bioreactor system thereof
  • Microalgae culture method and photo-bioreactor system thereof
  • Microalgae culture method and photo-bioreactor system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0049] Example 1 The three-step culture method and the application of nitrogen stress in the culture of Chaetoceros Mounerii.

[0050] The formula of the culture solution used in the present invention:

[0051] Improved f / 2 medium formula:

[0052] Nutrients

Concentration (mg / L)

NaNO 3

37.5

Urea

15

Na 2 SiO 3 .9H 2 O

30

NaH 2 PO 4 .2H 2 O

5.66

[0053] Nutrients

Concentration (mg / L)

Na 2 HPO 4

5.15

FeCl 3 .6H 2 O

3.162

Na 2 .EDTA

3.419

CuSO 4 .5H 2 O

0.01

ZnSO 4 .7H 2 O

0.023

CoCL 2 .6H 2 O

0.012

MnCL 2 .4H 2 O

0.18

Na 2 MoO 4 .2H 2 O

0.07

VB1

0.1

VB12

0.5×10 -3

Biotin

0.5×10 -3

MgCl 2 .7H 2 O

7.5

Sea crystal (is a substance commonly used to simulate sea water, purchase

From Tianjin Zhongyan Marine Biological Science Co., Ltd.

30

[0054] Formula of Nitrogen Deficiency Culture Solution: NaH 2 PO 4 .2H 2 O 5.66mg / L, Na 2 HPO 4 5.15mg / L, FeCl 3 .6H 2 O 3.162mg / L, Na 2 .EDTA 3.419mg / L, sea crystal is 30mg / ...

Embodiment 2 3

[0064] Example 2 Application of the three-step culture method and nitrogen stress in the culture of Scenedesmus desert.

[0065] Scenedesmus deserticola (Scenedesmus deserticola) was purchased from Jinan University. The Scenedesmus desert was connected to the first-stage reactor, and BG11 medium was used to inoculate the algae liquid in the logarithmic growth phase (the inoculation ratio was 5:1), and the initial OD 750 Is 0.583. Cultivation is conducted under continuous light, the light is the light beam designed by the present invention, the light intensity is 126±10umol / (m2.s), and the culture temperature is 25±5°C. Air and CO are introduced from the bottom of the first-stage reactor through the aeration head 2 Mixed gas, ventilation rate is 2.5L / min, CO 2 The percentage content of 5% is 5%, and the continuous cultivation time is 10 days. See the specific growth situation Figure 7 . In the first-stage reactor, using semi-continuous culture, by monitoring the OD value at 7...

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Abstract

The invention provides a novel photo-bioreactor system for culturing microalgae, and a method for culturing the microalgae by using the photo-bioreactor system. The method adopts different photo-bioreactors at different stages for culturing the microalgae, and the design of the photo-bioreactor of each stage reasonably takes the optimal culture condition of the microalgae at the stage into consideration so as to achieve the high-density culture and the product rapid accumulation of the microalgae, so a novel process and novel devices for the scale cultivation of the microalgae are provided.

Description

Technical field [0001] The present invention relates to the field of microalgae cultivation, in particular to a method for cultivating microalgae with a novel photobioreactor system and a novel photobioreactor system used in the method. Background technique [0002] In the current situation that resources and the environment are facing a double crisis, find an efficient renewable resource while achieving CO 2 Emission reduction becomes particularly important. Microalgae is one of the primitive plants with the highest photosynthetic efficiency. Compared with crops, the yield per unit area can be dozens of times higher. In addition, microalgae are rich in active substances such as proteins, polysaccharides, pigments, etc., which can be widely used in feed, food, medicines and health products, cosmetics, renewable fuels and other fields. After entering the 21st century, oil prices have soared rapidly, and the research on microalgae has quickly become a hot spot, and the technology ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12M1/00C12R1/89
CPCA01H4/001C12M23/34A01G33/00C12N1/12C12M23/58C12M21/02C12M31/10C12M23/00C12M35/08
Inventor 韩春梅李静张蕊刘敏胜
Owner ENN SCI & TECH DEV
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