Microalgae culture method and photo-bioreactor system thereof

A technology for photobioreactor and microalgae cultivation, which is applied in the field of new photobioreactor system, can solve the problems of many reactors, cumbersome liquid-liquid transportation, and no supplementary light system, so as to achieve rapid biomass accumulation and prevent light intensity Insufficient, simple and convenient operation

Active Publication Date: 2012-03-07
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the technical solution of this invention does not have a supplementary light system, so it is difficult to ensure the rapid growth of microalgae in the first-stage reactor, and there are many reactors, the liquid-liquid transportation between the reactors is cumbersome, and the energy consumption is large

Method used

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  • Microalgae culture method and photo-bioreactor system thereof
  • Microalgae culture method and photo-bioreactor system thereof
  • Microalgae culture method and photo-bioreactor system thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0049] Example 1 Application of three-step culture method and nitrogen stress in the cultivation of Chaetoceros mouroii.

[0050] The culture fluid formula used in the present invention:

[0051] Modified f / 2 medium formula:

[0052] Nutrients

Concentration (mg / L)

NaNO 3

37.5

urea

15

Na 2 SiO 3 .9H 2 o

30

NaH 2 PO 4 .2H 2 o

5.66

Na 2 HPO 4

5.15

FeCl 3 .6H 2 o

3.162

Na 2 .EDTA

3.419

CuSO 4 .5H 2 o

0.01

ZnSO 4 .7H 2 o

0.023

CoCL 2 .6H 2 o

0.012

MnCL 2 .4H 2 o

0.18

Na 2 MoO 4 .2H 2 o

0.07

VB1

0.1

VB12

0.5×10 -3

Biotin

0.5×10 -3

MgCl 2 .7H 2 o

7.5

Sea crystal (is a substance commonly used to simulate seawater, purchased

From Tianjin China Salt Marine Biological Science Co., Ltd.

30

[...

Embodiment 2 3

[0063] Example 2 Application of three-step culture method and nitrogen stress in the cultivation of Scenedesmus desertum.

[0064] Scenedesmus deserticola was purchased from Jinan University. Insert the Scenedesmus desertus into the primary reactor, use BG11 medium to inoculate the algae liquid in the logarithmic growth phase (the ratio of inoculation is 5:1), and the initial OD 750 is 0.583. Cultivate under continuous light, the light is the light beam designed by the present invention, the light intensity is 126±10umol / (m2.s), and the culture temperature is 25±5°C. Air and CO are introduced from the bottom of the primary reactor through the aeration head 2 The mixed gas, the ventilation volume is 2.5L / min, CO 2 The percentage content is 5%, and the continuous culture time is 10 days. For details on growth, see Figure 7 . In the primary reactor, semi-continuous culture is adopted. By monitoring the OD value at 750nm, it can be seen that the biomass of algae cells can...

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PUM

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Abstract

The invention provides a novel photo-bioreactor system for culturing microalgae, and a method for culturing the microalgae by using the photo-bioreactor system. The method adopts different photo-bioreactors at different stages for culturing the microalgae, and the design of the photo-bioreactor of each stage reasonably takes the optimal culture condition of the microalgae at the stage into consideration so as to achieve the high-density culture and the product rapid accumulation of the microalgae, so a novel process and novel devices for the scale cultivation of the microalgae are provided.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to a method for cultivating microalgae with a novel photobioreactor system and the novel photobioreactor system used in the method. Background technique [0002] In the current situation where resources and the environment are facing a double crisis, it is necessary to find an efficient renewable resource while achieving CO 2 Emission reduction becomes even more important. Microalgae is one of the original plants with the highest photosynthetic efficiency, and the yield per unit area can be dozens of times higher than that of crops. Moreover, microalgae are rich in active substances such as proteins, polysaccharides, and pigments, and can be widely used in feed, food, medical and health products, cosmetics, renewable fuels, and other fields. After entering the 21st century, the price of oil soared rapidly, and the research on microalgae quickly became a hot spot, and the tech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12M1/00C12R1/89
CPCA01H4/001C12M23/34A01G33/00C12N1/12C12M23/58C12M21/02C12M31/10C12M23/00C12M35/08
Inventor 韩春梅李静张蕊刘敏胜
Owner ENN SCI & TECH DEV
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