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Ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp

A fiber and oil technology, applied in the field of cellulosic algae and their cultivation applications, can solve the problems of not involving oil content, affecting the growth of algae cells, and bacterial contamination, so as to solve the oil source, avoid the inhibition of photosynthesis, and achieve a fast growth rate. Effect

Active Publication Date: 2016-06-29
CHINA PETROLEUM & CHEM CORP +1
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  • Description
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Problems solved by technology

CN102703326A discloses a high CO 2 Microalgae with tolerance and fixation rate and their breeding methods, but the algae strain provided by this patent does not involve the oil content of the algae strain
[0005] Liu Pinghuai et al. (The Effect of Organic Carbon Sources on Cell Growth, Oil Accumulation and Photosynthesis of Monospina, Bioengineering, 2012, 33(18):224-246) introduced a production method of using organic carbon sources to cultivate Monostipola , although the biomass exceeds 10g / L at the end of the culture, this method is a heterotrophic culture method of Monostipella. During the culture process, organic carbon sources such as glucose are used to achieve cell growth. This culture method does not use CO 2 Other inorganic carbon sources are economical, and the addition of organic carbon sources is prone to bacterial contamination during the cultivation process, which affects the growth of algae cells

Method used

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  • Ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp
  • Ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp
  • Ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp

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Experimental program
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Effect test

Embodiment 1

[0021] Example 1 Separation and acclimatization screening to obtain Monostipella SS-B7

[0022] (1) Obtaining the starting algae strain: In October 2011, 50 mL of water samples were retrieved from Longhu Lake in Shoushan Leisure Resort, Dubolte Mongolian Autonomous County, Heilongjiang, and inoculated into 200 mL of BG11 medium for enrichment culture. The light intensity for cultivation is 5000Lux, the temperature is 25°C, the light-dark cycle is 24h, and the light-dark time ratio is 14:10. After about half a month of cultivation, the culture medium turns green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000Lux and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cultivation, the cultivation temperature is 25°C, and the light...

Embodiment 2

[0027] Identification of embodiment 2 algal strains

[0028] The DNA of the obtained SS-B7 algal cells was extracted by CTAB method, and the 18SrDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18SrDNA gene sequencing analysis are shown in the sequence list. The 18SrDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Ankistrodes mussp. There is the greatest similarity, its BLASTn value is 2702, Maxindex value is 99%, it can be determined that SS-B7 is a cellulosic algae ( Ankistrodes mussp. ).

Embodiment 3

[0029] The cultivation application of embodiment 3 cellulosus SS-B7

[0030] The algae liquid of SS-B7 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formulation of BG11 medium is shown in Table 1 and Table 2. The cultivation was carried out in a photobioreactor. The OD of the culture solution after inoculation was 690 is 0.2. CO was introduced from the bottom of the reactor 2 Flue gas with a content of 30v%, of which NO 2 The content is 300×10 -6 (v / v). During the cultivation process, the light intensity was 5000Lux, the cultivation temperature was 28°C, the pH value was controlled between 7-9, the light cycle was 24h, the light-dark time ratio was 14:10, and the cultivation time was 8 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, vacuum freeze-dried to constant weight at -60°C, and then the dry weight of the algae powder was measured to calculate the biomass production, and th...

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Abstract

The invention discloses ankistrodesmus sp containing lipid as well as culture and applications of ankistrodesmus sp, wherein the ankistrodesmus strain is SS-B7, the classification name of SS-B7 is Ankistrodesmus sp, and the ankistrodesmus sp SS-B7 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 15, 2013, and has the preservation number CGMCC No.7478. The ankistrodesmus sp SS-B7 has tolerance to CO2 and NOx with high concentrations, and has high carbon sequestration efficiency, the content of total lipids in the cells of the obtained biomass accounts for 40% or above of the dry cell weight, and the ankistrodesmus sp SS-B7 can be used for producing biodiesel.

Description

technical field [0001] The invention belongs to the field of biotechnology and bioenergy, in particular to a strain tolerant to high concentration CO 2 and NO X Lipid-rich cellulosic algae and their cultural applications. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion ef...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64B01D53/84B01D53/62B01D53/56C12R1/89
CPCY02A50/20Y02P20/59
Inventor 师文静廖莎姚新武樊亚超孙启梅王领民高大成李晓姝
Owner CHINA PETROLEUM & CHEM CORP
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