Method for producing biodiesel from lignocellulose

A lignocellulose and biodiesel technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of competition with people for food and high cost of biodiesel, and achieve the effect of reducing production costs

Inactive Publication Date: 2013-01-09
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The existing production of biodiesel has many probl

Method used

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  • Method for producing biodiesel from lignocellulose
  • Method for producing biodiesel from lignocellulose
  • Method for producing biodiesel from lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: straw pretreatment experiment

[0031] 1. Roll the straw into small pieces and crush it into 40-mesh powder with a pulverizer.

[0032] 2. Weigh 1.5g straw powder into the sealed tube, add NaOH and Ca(OH) in proportion (mass ratio) 2 , and add water according to the solid-to-liquid ratio.

[0033] 3. Treat at 100°C for 2 hours.

[0034] 4. After cooling, adjust the pH to neutral with sulfuric acid, and filter it with a filter dry pan. After suction filtration, wash with water several times and discard the filtrate. Dry the pan and dry the treated straw powder at 80°C until constant weight.

[0035] 5. Weigh 100 mg of the treated straw powder into the enzymatic hydrolysis bottle, and add 2 mL of 0.05mol / L pH=5.47 Citric acid buffer. Add 25 U ctec (cellulase from Novozymes) to 1 g of solid, and enzymatically hydrolyze at 50°C for 24 hours. The blank control is 2mL 0.05mol / L pH=5.47 Citric acid buffer without adding substrate, only adding an equal amoun...

Embodiment 2

[0042] Embodiment 2: 5L fermentor fermentation Clostridium thermocellum

[0043] 1. According to the recipe "KH 2 PO 4 1.0g / L, K 2 HPO 4 (anhydrous) 6g / L, urea 17.8g / L, MgCl 2 ·6H 2 O 2.49g / L, CaCl 2 2H 2 O 135mg / L, FeSO 4 ·6H 2 O 5mg / L, Cysteine ​​hydrochloride 2.0g / L, Resazurin 2.0mg / L, cellobiose 30.0g / L, Yeast extract 12.0g / L, Sodium citrate 2H 2 O 0.752g / L" to prepare fermentation medium (calcium, magnesium, iron salt mother solution added after sterilization), 115 ℃, 15min moist heat sterilization.

[0044] 2. After the sterilization is completed, nitrogen gas is continuously fed into the fermenter to remove the oxygen in the medium and the fermenter. After the medium changes from light blue or pink (the color of resazurin) to its own color, adjust the pH of the medium to 7.0, keep the tank temperature at 60°C, and add the required volume of JYT01 primary seed solution at the same time , stop aeration, stir and ferment at 80rpm.

[0045] 3. After about 48 ho...

Embodiment 3

[0046] Example 3: CBP combined with Schizochytrium heterotrophic fermentation to produce biodiesel

[0047] Adopt above embodiment gained Clostridium thermocellum to produce sugar liquid (carbon source content 10%), add yeast extract 3% additionally, vitamin B1, 40mg / L; Vitamin B6, 20mg / L; Vitamin B12, 10mg / L; Sodium (vitamin H) 10mg / L. Fermentation was carried out under the following conditions: temperature 25°C, ventilation volume 1.0L min-1 L-1 (VVM), tank pressure 0.06MPa, stirring speed 200rpm. The pH is controlled to be 6.0, and the fermentation time is 96h.

[0048] After the fermentation, the yield of Schizochytrium was 44.15g / L obtained from the 5L fermenter, the bio-oil was 23.8g / L, and the DHA content was 9g / L (Table 2, 3).

[0049] Table 2 Component analysis results of Schizochytrium sp.

[0050]

[0051] Table 3 Fatty acid analysis results of Schizochytrium fermented by CBP

[0052]

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Abstract

The invention belongs to the field of microbial fermentation engineering, and particularly relates to a technology of consolidated bioprocessing (consolidated bioprocessing, CBP). Cellulose is fermented and degraded by a cellulose degradation strain (such as clostridium thermal fiber) to generate saccharides and organic acids, such as glucose, lactic acid and acetic acid; and biodiesel and co-produced products with high additional values, such as timnodonic acid, docosahexenoic acid and protein feed, are produced by serial fermentation combined with large-scale heterotrophism or raising cultivation of microalgae (such as schizochytrium limacinum, chlorella and nannochloropsis oculata). The method has the characteristics that anaerobic cellulose degradation bacteria are utilized to degrade the preprocessed lignocellulose material, so that the cellulose and hemicelluloses are converted into carbon sources which can be utilized by the microalgae such as soluble saccharide and organic acids; the carbon sources are inoculated into a microalgae seed liquid to be fermented after a certain nitrogen source and other nutrient elements are added, and oil is accumulated to generate the biodiesel and other products with high additional values.

Description

technical field [0001] The present invention relates to the field of microbial fermentation engineering, specifically utilizing integrated bioprocessing (Consolidated Bioprocessing, CBP) technology to establish a cellulose-degrading strain (such as Clostridium thermocellum) to ferment and degrade cellulose to produce sugars and organic acids such as glucose and lactic acid , acetic acid, etc., combined with large-scale heterotrophic or combined culture of microalgae (such as Schizochytrium and Nannochloropsis), fermented to produce biodiesel and products with high added value such as eicosapentaenoic acid (Eicosapentaenoic Acid, EPA) and docosahexaenoic acid (Docosahexaenoic acid, DHA) in series fermentation process. Background technique [0002] With the intensification of the global energy and environmental crisis, seeking alternative energy has become a research hotspot in many countries, among which clean and renewable bio-energy is an important aspect of alternative ene...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P19/00C12P7/40C12R1/145C12R1/89
CPCY02E50/13Y02E50/10
Inventor 崔球宋晓金朱新术高莽刘亚君
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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