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Schizochytrium limacinum strain for producing docosahexaenoic acid

A technology of docosahexaenoic acid and Schizochytrium, applied in the field of bioengineering, can solve problems such as yield gap and achieve the effect of less by-products

Inactive Publication Date: 2013-01-09
广东丝恩食品工业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, domestic research institutions and universities have also begun to study Schizochytrium (Schizochytrium) Research on the production of DHA by fermentation, but there is still a big gap in yield and quality compared with developed countries

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Analysis of Fatty Acid Composition of Schizochytrium

[0032] Schizochytrium Schizochytrium limacinum JN168 adopts liquid fermentation, and the medium composition is: glucose 20g, peptone 10g, yeast extract 5g, sea crystal 20g, distilled water 1000mL, pH7.0. The fermentation temperature was 25°C, and the fermentation ended after 5 days. The mycelium was collected by suction filtration, freeze-dried to constant weight, and the mycelium was ground with a mortar. Take about 0.1g of dried mycelium and add it to a test tube with a cover. Add 5mL of 0.4mol / L potassium hydroxide-methanol solution to it, keep it in a water bath at 50°C for 1h, then add 5mL of 14% boron trifluoride-methanol solution, keep it in a water bath at 50°C for 1h, add 5mL of n-hexane Extract with alkane, and finally add 2 mL of saturated sodium chloride solution to wash with water, pipette the upper layer solution and use GC-MS for detection.

[0033] Chromatographic conditions: Finnigan Trace MS ...

Embodiment 2

[0038] Determination of the best carbon source

[0039] Transfer the strains stored at -70°C to a plate containing an appropriate amount of seawater, add an appropriate amount of UV-sterilized pine pollen, and culture at 25°C for 4 days. Take 3mL of the activated bacterial solution, insert it into a 250mL shaker flask containing 50mL of seed medium, and culture it on a shaking table at 25°C and 200r / min for 2 days. The seed solution was inoculated into the fermentation medium with a 4% inoculum amount, and cultured on a shaker at 25°C and 200r / min for 72 hours. Using a single factor to investigate the best carbon source for docosahexaenoic acid accumulation, use fructose, maltose, sucrose, lactose, glycerol, and soluble starch to replace glucose in the basic fermentation medium for liquid fermentation, and measure the dry weight and 20 Dicosahexaenoic acid production. Basic fermentation medium composition (w / v): glucose 90, yeast powder 10, sea crystal 20, pH 7.0, sterilized...

Embodiment 3

[0043] Determination of the best nitrogen source

[0044] Transfer the strains stored at -70°C to a plate containing an appropriate amount of seawater, add an appropriate amount of UV-sterilized pine pollen, and culture at 25°C for 4 days. Take 3mL of the activated bacterial solution, insert it into a 250mL shaker flask containing 50mL of seed medium, and culture it on a shaking table at 25°C and 200r / min for 2 days. The seed solution was inoculated into the fermentation medium with a 4% inoculum amount, and cultured on a shaker at 25°C and 200r / min for 72 hours. Using a single factor to investigate the best nitrogen source for docosahexaenoic acid accumulation, use ammonium sulfate, ammonium nitrate, yeast extract, peptone, and corn steep liquor to replace the yeast powder in the basic fermentation medium for liquid fermentation, and measure the dry weight and Docosahexaenoic acid production. Basic fermentation medium composition (w / v): glucose 90, yeast powder 10, sea crys...

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Abstract

The invention relates to a schizochytrium limacinum strain for producing docosahexaenoic acid and belongs to the technical field of bioengineering. The strain is named as slug type schizochytrium limacinum JN168 and preserved at China Center for Type Culture Collection, and the preserving number is CCTCCNO: M2012074. By performing separation screening on water samples of the Yellow Sea water areaof Qingdao, Shandong Province, the schizochytrium limacinum strain which is used for producing docosahexaenoic acid (DHA) and is stable in characteristics is obtained, and high yield of docosahexaenoic acid can be achieved by liquid state or solid state fermentation at 25-30 DEG C.

Description

technical field [0001] A Schizochytrium strain producing docosahexaenoic acid belongs to the technical field of bioengineering. The invention relates to a Schizochytrium strain with high yield of docosahexaenoic acid. The strain has fast growth speed and high yield, and is of great significance for the industrial production of docosahexaenoic acid. Background technique [0002] Docosahexaenoic acid, referred to as DHA in English, is an important member of the n-3 series of polyunsaturated fatty acids, and the system name is all-cis △-4,7,10,13,16,19-docosahex Enenoic acid. Due to the special structure containing five unsaturated bonds, DHA has special functions and important influences on human health. DHA mainly exists in the human cerebral cortex and retina. Studies have shown that DHA plays an important role in preventing cerebral thrombosis, promoting brain synthesis in infants, intellectual development of infants, anti-inflammation, enhancing human immunity, and anti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/64C12R1/01
Inventor 蔡宇杰丁彦蕊周立树金大勇朱建航廖祥儒张大兵李枝玲罗军侠
Owner 广东丝恩食品工业有限公司
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