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Method for promoting synthesis of DHA in schizochytrium limacinum grease

A technology of Schizochytrium and oil, which is applied in the field of promoting the synthesis of docosahexaenoic acid in Schizochytrium oil, which can solve the problem of increased DHA unsaturated fatty acid content, space limitation of DHA content increase, saturated fatty acid Increased DHA content and other problems, to achieve the effect of increasing DHA content, promoting a large amount of synthesis, and improving quality

Active Publication Date: 2015-03-25
WUHAN HUASHITE IND BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a certain improvement on the technology of producing DHA by Schizochytrium fermentation. However, the addition of acetic acid, citric acid and other substances not only leads to the increase of unsaturated fatty acid content including DHA, but also leads to the increase of saturated fatty acid content. As a result, the space for increasing DHA content is limited

Method used

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  • Method for promoting synthesis of DHA in schizochytrium limacinum grease
  • Method for promoting synthesis of DHA in schizochytrium limacinum grease
  • Method for promoting synthesis of DHA in schizochytrium limacinum grease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Add 50 mL of seed medium to a 250 mL Erlenmeyer flask. Insert the low-temperature frozen schizochytrium strains, cultivate them for 2 days at a culture temperature of 25°C and a shaker rotation speed of 200rpm, and activate the low-temperature frozen schizochytrium strains into Schizochytrium seed liquid;

[0029] Seed medium components (g / L): glucose 15.0, yeast powder 6.0, beef extract 3.0, peptone 2.0, corn steep liquor 4.0, MgSO 4 ·7H 2 O 0.2, KH 2 PO 4 2.0, Sea Crystal 15.0.

[0030] (2) Add 50 mL of fermentation medium to a 250 mL Erlenmeyer flask, insert Schizochytrium into the fermentation medium with a 10% inoculum size, and culture at a constant temperature of 26° C. for 3 days at a rotating speed of 200 rpm;

[0031] Fermentation medium components (g / L): glucose 60.0, yeast extract 6.0, MgSO 4 ·7H 2 O0.6, KH 2 PO 4 0.5, NaNO 3 1.0, Na 2 SO 4 5.0;

[0032] After the fermentation medium is sterilized, the exogenous regulatory factor sesamol is a...

Embodiment 2

[0047] (1) Add 50 mL of seed medium to a 250 mL Erlenmeyer flask. Insert the cryopreserved Schizochytrium strains, culture them for 2 days at a culture temperature of 25°C and a shaker rotation speed of 200rpm, and activate the cryopreserved Schizochytrium strains into Schizochytrium seed liquid;

[0048] Seed medium components (g / L): glucose 25.0, yeast powder 8.0, peptone 5.0, corn steep liquor 2.0, MgSO 4 ·7H 2 O 0.6, KH 2 PO 4 1.5, sea crystal 10.0.

[0049] (2) Add 50 mL of fermentation medium to a 250 mL Erlenmeyer flask, insert Schizochytrium into the fermentation medium with an inoculum size of 8%, and culture at a constant temperature of 26° C. for 3 days at a rotating speed of 200 rpm;

[0050] Fermentation medium components (g / L): glucose 50.0, yeast extract 10.0, sodium glutamate 5.0, MgSO 4 ·7H 2 O 0.2, KH 2 PO 4 2.0, (NH 4 ) 2 SO 4 3.0, NaNO 3 2.0, Na 2 SO 4 8.0;

[0051] After the fermentation medium is sterilized, the exogenous regulatory factor ...

Embodiment 3

[0061] (1) Add 50 mL of seed medium to a 250 mL Erlenmeyer flask. Insert the cryopreserved Schizochytrium strains, culture them for 2 days at a culture temperature of 25°C and a shaker rotation speed of 200rpm, and activate the cryopreserved Schizochytrium strains into Schizochytrium seed liquid;

[0062] Seed medium components (g / L): glucose 30.0, yeast powder 4.0, beef extract 5.0, corn steep liquor 6.0, MgSO 4 ·7H 2 O 1.0, KH 2 PO 4 0.5, sea crystal 20.0.

[0063] (2) Add 50 mL of fermentation medium to a 250 mL Erlenmeyer flask, insert Schizochytrium into the fermentation medium with an inoculum size of 4%, and culture at a constant temperature of 26° C. for 3 days, with a rotation speed of 200 rpm;

[0064] Fermentation medium components (g / L): glucose 40.0, yeast extract 15.0, sodium glutamate 10.0, MgSO 4 ·7H 2 O 1.0, KH 2 PO 4 1.5, (NH 4 ) 2 SO 4 5.0, NaNO 3 3.0, Na 2 SO 4 12.0;

[0065] After the fermentation medium was sterilized, the exogenous regulat...

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Abstract

The invention relates to a method for promoting synthesis of DHA in schizochytrium limacinum grease, and particularly relates to a method for prompting synthesis of docosahexaenoic acid (DHA) in schizochytrium limacinum grease. The method is characterized in that the activity of malic enzyme in a tricarboxylic acid transport system is inhibited by adding an exogenous control factor, so that the content of the NADPH in the schizochytrium limacinum can be greatly reduced, the synthesis of a great amount of DHA can be promoted, and the exogenous control factor is sesamol. According to the method, a fermentable carbon source also can be selected to reduce metabolic flux of a pentose phosphate pathway in the bacteria, so that the content of NADPH in the schizochytrium limacinum can be greatly reduced, the synthesis of a great amount of DHA can be promoted, and the largest content of the DHA in the grease of final bacteria can reach 55.3 percent. The method is simple, easy, obvious in effect, capable of greatly increasing the content of DHA in the bacteria grease and is likely to realize the industrialized application.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for promoting the synthesis of docosahexaenoic acid (DHA) in Schizochytrium oil. Background technique [0002] Docosahexaenoic acid (DHA) belongs to the series of omega-3 polyunsaturated fatty acids. It has very important physiological functions in the human body. It is an important functional nutrient that the human body cannot synthesize by itself but is indispensable. The research found that DHA exists in large quantities in the nervous tissue of the human cerebral cortex and the retina. It is an important fatty acid component in the brain and visual cells. It can promote brain development, improve intelligence and improve memory. In addition, it can also prevent the deposition of cholesterol on the blood vessel wall, prevent or reduce the occurrence of atherosclerosis and coronary heart disease; there are also research evidences that DHA can effectively induce t...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427
Inventor 余龙江陈伟朱圆敏金文闻余金龙
Owner WUHAN HUASHITE IND BIOTECH DEV
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