27 results about "Pentose phosphate pathway" patented technology

The invention discloses a method for promoting synthesis of microbial oil based on a light-operated gene and a photocatalyst, and belongs to the technical field of microbial oil. According to the invention, the oleaginous yeast Po1f-OptoZWF1 containing a light-operated gene expression system is constructed by adopting a molecular biological technology, and meanwhile, the oleaginous yeast is cultured by adopting a two-stage method (darkness and illumination). In the dark stage, sufficient nutrition is provided, and rapid proliferation of the oleaginous yeast is promoted; in the grease accumulation stage, illumination culture is converted, a photocatalyst is added, a photocatalyst-oleaginous yeast hybrid system and a light-operated gene expression system are constructed to inhibit the ZWF1 gene and a corresponding pentose phosphate pathway, and a photocatalytic material provides photo-induced electrons to promote regeneration of reducing power NADPH, so that grease synthesis is efficiently promoted. According to the method, through gene modification and a two-stage culture strategy, the yield of oil synthesis is remarkably increased, and the problems that existing oil-producing microorganisms are slow in proliferation and low in oil yield are solved.

The invention discloses a method for efficiently synthesizing D-psicose from cane sugar by escherichia coli through double PTS transfer. E.coliJM109 (DE3) is used as a chassis host bacterium, and a D-psicose synthesis path based on a sucrose PTS transport system is established through co-expression of alsE, a6PP, scrA and scrB genes; by introducing the glpX [K29A] gene, a carbon source is driven to flow from a D-fructose PTS transport system to a D-psicose synthesis path; through knockout of pfkA and rpiA genes and inhibition of expression of fbaA genes, an endogenous glycolysis pathway and a phosphopentose pathway are adjusted, and the carbon flow entering tricarboxylic acid circulation is adjusted, and finally, the purpose that the D-psicose is efficiently synthesized from sucrose by escherichia coli through double PTS transport is finally achieved. Genetic engineering modification is carried out on wild escherichia coli, D-psicose is efficiently synthesized from sucrose by using a sucrose PTS and fructose PTS system, and a new scheme is provided for synthesis of D-psicose.

The invention belongs to the technical field of molecular biology and genetic engineering, and discloses a CRISPR / dCas9 vector for improving the yield of lactyl-N-tetrasaccharide of bacillus subtilis as well as a construction method and application of the CRISPR / dCas9 vector, and a CRISPR / dCas9 system is applied to production of lactyl-N-tetrasaccharide from bacillus subtilis 168 for the first time. According to the invention, pW-dcas9, pW-sgRNA and pW-sgRNA-zwf recombinant vectors are constructed, the transcriptional level of the zwf gene in a phosphopentose pathway is significantly reduced by 11.32 times by using a CRISPR / dCas9 system induced by xylose, and the metabolic flux of a competitive pathway is weakened. The yield of the lactyl-N-tetrasaccharide of the engineering strain obtained by the invention is greatly improved by 31% and reaches 7.25 g / l. Meanwhile, an effective method is provided for lowering metabolic flows of a cell wall competitive pathway and a glycolysis competitive pathway in a later period.

The invention relates to the technical field of synthetic biology, in particular to a squalene high-yield saccharomyces cerevisiae engineering bacterium and a construction method and application thereof.Saccharomyces cerevisiae CEN.PK.2-1D is used as an original strain, pentose phosphate pathway genes ZWF1, GND1, TAL1 and TKL1 and IDP1 genes of TCA circulation are integrated in a genome of the original strain through homologous recombination, and the squalene high-yield saccharomyces cerevisiae engineering bacterium is obtained. Constructing a dual-channel cofactor supply system to improve the intracellular NADPH level and the NADPH / NADP + ratio; according to the saccharomyces cerevisiae genetic engineering strain Sc-Sq04 constructed by the method disclosed by the invention, the yield of squalene obtained by shake flask fermentation reaches 741.08 + / -28.9 mg / L by further integrating all genes of an MVA way, ERG20 and ERG9 genes, and a new thought is provided for transforming saccharomyces cerevisiae to synthesize terpene compounds and promoting industrial application.

The invention provides a genetically engineered bacterium for efficiently producing pseudouridine and application of the genetically engineered bacterium. The genetically engineered bacterium is based on a high-yield uridylic acid strain E. coli MB219 (the patent application number is 202410153359.6), and the high-efficiency pseudouridine engineering bacterium is constructed through systemic gene modification. The genetically engineered bacterium is characterized in that (1) gene knockout: 10 genes such as pbs1, psuK and the like are knocked out to block a metabolic pathway of byproducts; (2) metabolism regulation: replacing a J23110 strong promoter, and overexpressing zwf / gnd to enhance a phosphopentose pathway; (3) heterologous gene integration: integrating a specific dephosphorylase gene HDHD1, and introducing RjpsuG, NmygdH and thyA to construct a co-expression plasmid pRL06; the fermentation yield of the finally obtained engineering bacterium CY20 (pRL06) reaches 45.3 g / L and is increased by 6 times compared with that of a traditional method, the production efficiency is greatly improved, the product purity is high, and by-products are few.

The invention provides an application of lurasidone or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and / or treating diabetes individual bone injury healing disorder. The hyperglycemia environment causes excessive secretion of lipocalin-2 (LCN2) by activating a phosphopentose pathway of neutrophil, thereby inhibiting osteogenic differentiation of skeletal stem cells. According to the application of the lurasidone or the pharmaceutically acceptable salt thereof in the preparation of the medicine for preventing and / or treating the diabetic individual bone injury healing disorder, in the application provided by the invention, the lurasidone can specifically inhibit the metabolic pathway and the secretion of LCN2, remodel an immune microenvironment beneficial to repair, and promote the healing of the diabetic individual bone injury. The formation of callus under the condition of diabetes mellitus is obviously accelerated and the mineral density of bone is improved.

The present invention belongs to the field of microbial technology, and relates to a genetically engineered bacterium for preparing ethanol based on hydrolyzed sugar from wood fiber and its application. The bacterium is a high xylose utilization and inhibitor tolerance engineered bacterium. The engineered bacterium is based on Saccharomyces cerevisiae S288C as a chassis strain, and co-expresses heterologous xylose reductase-xylitol dehydrogenase pathway-related genes, xylose transport-related genes, xylose isomerase-related genes and xylose isomerase genes, and over-expresses endogenous xylulose kinase-related genes, non-oxidative pentose phosphate pathway-related genes, reduced glutathione system genes, superoxide dismutase expression system-related genes, NADPH / NADP + The genes and transcription factors related to the cofactor circulation system were knocked out, the genes related to the competitive metabolic pathway were knocked out, and the bacteria were domesticated with sugar sources and inhibitors as pressure; the engineered bacteria can efficiently metabolize xylose and produce high-concentration ethanol in non-detoxified lignocellulose hydrolysate with high inhibitor concentrations and without any nutrient salt addition.

The present invention relates to a variant microorganism for producing 1,3-propanediol and a 1,3-propanediol production method using same. More specifically, genes of a microorganism are modified and recombined to produce 1,3-propanediol, by improving the citric acid cycle (TCA cycle) and the glycoxylate pathway in a strain having improved pentose phosphate (PP) and Entner-Doudoroff (ED) pathways. Therefore, a high concentration of 1,3-propanediol may be produced by using the variant microorganism according to the present invention.

This invention discloses an engineered bacterial strain for producing xylitol using glucose as the sole carbon source, its construction method, and its applications, belonging to the field of genetic engineering. Using *Escherichia coli* as the starting strain, this invention optimizes the metabolic pathway by knocking out the 2-keto-3-deoxy-6-phosphate glucuronide gene (eda), knocking out the 6-phosphoglucuronide dehydratase gene (edd), and weakening the glucose phosphoisomerase gene (pgi), thus concentrating the carbon flow in the pentose phosphate pathway. Furthermore, it optimizes the metabolic pathway by knocking out or overexpressing endogenous genes such as the ribulokinase gene (araB). Subsequently, the synthesis of xylitol from glucose was experimentally tested by expressing a recombinant expression plasmid containing 2-arabinol dehydrogenase, 4-arabinol dehydrogenase, and xylitol dehydrogenase. This invention, through optimized metabolic pathways, efficiently converts glucose to xylitol without the need for glycerol addition, and significantly increases the yields of both the precursor arabinol and the product xylitol, demonstrating promising production prospects.

The present invention relates to a mutant microorganism for producing 1,3-propanediol and a 1,3-propanediol production method using same. Specifically, the present invention has been recombined to enable producing 1,3-propanediol by enhancing the pentose phosphate (PP) pathway and the Entner-Doudoroff (ED) pathway by manipulating genes of the microorganism. Thus, by using the mutant microorganism according to the present invention, a high concentration of 1,3-propanediol may be produced.

This invention relates to mutant microorganisms for producing 1,3-propanediol and a method for producing 1,3-propanediol using the same. Specifically, the invention involves recombining the genes of microorganisms to enhance the TCA cycle and glycoxylate pathway in strains that strengthen the PP pathway (pentose phosphate pathway) and ED pathway (Entner-Doudoroff pathway), thereby enabling the production of 1,3-propanediol. Therefore, high concentrations of 1,3-propanediol can be produced using the mutant microorganisms according to the invention.

This application relates to the fields of bioengineering and fermentation technology, and discloses a genetically engineered *Escherichia coli* strain that synthesizes L-histidine and its preparation method. The engineered strain uses *Escherichia coli* W3110 as the starting strain and is modified... hisG Gene release from feedback inhibition; knockout ushA and nrdD Genes reduce precursor consumption; inactivation slyA Genes reduce energy waste; heterogeneous integration zwf and gnd Genes and replacement pgi The gene promoter redirects carbon flow to the pentose phosphate pathway, enhancing the supply of key precursors PRPP and reducing agent NADPH; introducing courtE Genes promote product efflux. Combined with an optimized fed-batch fermentation process, this invention effectively solves the problems of insufficient precursor supply, uneven energy distribution, and product inhibition through a systems metabolic engineering strategy, increasing L-histidine yield and sugar-acid conversion rate, making it suitable for industrial production.

The invention discloses a preparation method and an application of an iron-doped zeolite imidazole skeleton nano-drug delivery system (GOx / MTT coated Fe-ZIF-8) coated with glucose oxidase (GOx) and thiazole blue (MTT). The GOx / MTT coated Fe-ZIF-8 is decomposed to release Fe < 2 + >, GOx and MTT in an acidic environment of tumor cells. The released GOx further enhances the acid environment in the tumor while realizing hunger treatment, and damages the pentose phosphate pathway to cause insufficient supply of intracellular NADPH (Nicotinamide Adenine Dinucleotide Phosphate). Subsequently, the free MTT reacts with the NADPH to force the NADPH to be depleted and homocystine to be excessively uptake, so that disulfide in the cells is stressed to damage the activity and function of corresponding protein, and the disulfide death of the cells is caused. In addition, excessive H2O2 and Fe < 2 + > in cells are subjected to a Fenton reaction to generate a large amount of reactive oxygen species (ROS) to destroy intracellular oxidation-reduction balance, so that chemical kinetic treatment is realized. According to the nano-drug delivery system, targeted delivery of tumor cells is achieved, multi-mode collaborative treatment of hunger, disulfur death and chemical kinetics is achieved, toxic and side effects of treatment are reduced, and the tumor treatment effect is improved.

The application belongs to the technical field of traditional Chinese medicine and biological preparation, and discloses a Chinese yam compound peptide preparation, a preparation method and application of the Chinese yam compound peptide preparation in preparation of a medicine for invigorating the spleen and strengthening five internal organs. The preparation is a Chinese yam bioferment after fermentation and Chinese yam compound peptide powder. The Chinese yam compound peptide powder prepared by the application improves the whole body symptoms of a rat with hyperlipidemia of the spleen deficiency type through multi-target regulation of metabolic pathways, such as up-regulation of the phosphopentose pathway, promotion of glutathione metabolism, regulation of the nerve-intestine-axis and synergistic repair of organ functions, so as to achieve the effect of 'five internal organs synergistic repair' from'single spleen invigoration'. Preventive intervention (the protection group) reverses metabolic disorders more significantly than the treatment group by early regulation of glutamine metabolism and ROR alpha rhythm coordination, realizes multi-organ function repair, systematically improves the body damage caused by hyperlipidemia, and finally achieves the effect of invigorating the spleen and strengthening five internal organs, so as to achieve the effect of 'five internal organs synergistic repair' from metabolic regulation.

The invention belongs to the technical field of microbial fermentation and metabolic engineering, and discloses a recombinant strain for producing succinic acid through co-utilization of glucose and xylose as well as a construction method and application of the recombinant strain. The invention provides a genetically engineered bacterium, and the genetically engineered bacterium can simultaneously utilize and metabolize glucose and xylose by enhancing the activity or gene expression of xylose transporter of pichia kudriavzevii, gene expression involved in a xylose utilization pathway and gene expression involved in a phosphopentose pathway. Experiments prove that the genetically engineered bacterium can improve the co-utilization efficiency of xylose and glucose, has high sugar-acid conversion rate (up to 79.1%), and significantly improves the yield of succinic acid.

The invention provides a method for biologically synthesizing hydroxytyrosol from escherichia coli by using levodopa. The method comprises the following steps: by taking escherichia coli as a chassis host bacterium, constructing a way for biologically synthesizing hydroxytyrosol by taking levodopa as a substrate through overexpression of an alcohol reductase gene yqhD, a dopa decarboxylase gene dodc and a tyramine oxidase gene tyo; the method comprises the following steps: knocking out a phenylacetaldehyde oxidase gene feaB and a 4, 5-DOPA external diol dioxygenase gene ygiD, so as to reduce the generation of by-products; nusA tag protein is fused at the N end of tyramine oxidase gene tyo, and catalase gene KatG is overexpressed, so that a synthesis path of hydroxytyrosol is optimized; according to the present invention, the expression of the glucose-6-phosphate isomerase gene pgi is inhibited so as to adjust the carbon flux of the glycolysis pathway and the pentose phosphate pathway, such that the hydroxytyrosol biosynthesis pathway is reinforced, and the efficient hydroxytyrosol biosynthesis is finally achieved by using the levodopa; the invention provides a new scheme for biosynthesis of hydroxytyrosol.

The invention discloses a recombinant Yarrowia lipolytica engineering strain for producing chlorogenic acid and application of the recombinant Yarrowia lipolytica engineering strain, and belongs to the technical field of genetic engineering and bioengineering. According to the invention, a hydroxylation module and a condensation module are constructed in the Yarrowia lipolytica, heterologous synthesis of CGA is realized, supply of NADPH is promoted through optimization of an endogenous pentose phosphate pathway, oxygen intake is increased and a shikimic acid pathway is enhanced by using hemoglobin VHb of heterologous Vitreoscilla, and the yield of chlorogenic acid is further increased. The yield of chlorogenic acid of the constructed engineering bacterium can reach 13.13 g / L, a foundation is laid for subsequent biosynthesis of compounds such as chicoric acid, and the engineering bacterium has potential value and significance for development of synthetic biology.

Inhibitors directed at enzymes of the pentose phosphate pathway of protozoan parasites, e.g., Trypanosoma cruzi glucokinase, are described. Inhibitors are derivatives of gossypol (2,2′-Bis(formyl-1,6,7-trihydroxy-5-isopropyl-3-methylnaphthalene). The inhibitors can specifically target glucokinase and hexokinase of Trypanosoma cruzi (T. cruzi), Trypanosoma brucei (T. brucei), and Leishmania spp. and are useful in treatment of parasitic diseases including Chagas' disease and African sleeping sickness, among others.

The invention relates to the field of biological medicine, and provides application of a pentose phosphate pathway regulator in resisting platelet storage damage. According to the invention, a non-targeted metabonomics technology is utilized to systematically analyze the metabolic change of the singly-collected platelets during the in-vitro storage period. The relationship between the blood platelet storage damage and the blood platelet storage damage is discussed by intervening the significantly enriched metabolic pathway in the blood platelet storage process. Research finds that the pentose phosphate pathway (PPP) has a potential effect in regulating platelet storage damage, and a new research idea is provided for improving the platelet storage quality by intervening the platelet metabolism process.

The invention discloses escherichia coli capable of producing caffeic acid and application, and belongs to the technical field of biological engineering. According to the invention, a production strain E.coli Tyr10 with high yield of tyrosine shikimic acid is used as a chassis, zwf is knocked out to block a phosphopentose pathway, and tktA, rpe and ppnk are over-expressed to improve the yield of L-tyrosine; meanwhile, the yield of the caffeic acid is increased by heterologous expression of FjTAL and SsaTYR, and further, the yield of the caffeic acid further reaches 211 mg / L by performing protein engineering modification on the SsaTYR; finally, in order to reduce the toxicity of the caffeic acid to cells, the tyrosine ammonia lyase coding gene RtTAL is over-expressed and coded, the yield of the caffeic acid is increased to 471 mg / L, and the yield of the caffeic acid reaches 8.01 g / L when the caffeic acid is finally fermented for 38 h in a fermentation tank. The strain has the industrial application potential of fermentation production of caffeic acid.

The invention relates to the technical field of biological medicine, and discloses engineering living body photo-thermal hydrogel as well as a preparation method and application thereof. The engineering living body photo-thermal hydrogel comprises a sodium alginate hydrogel matrix loaded with polypyrrole nanoparticles, and gene engineering modified escherichia coli entrapped in the sodium alginate hydrogel matrix, wherein the gene engineering modified escherichia coli expresses lactate oxidase or glucose oxidase. According to the engineered living body photo-thermal hydrogel provided by the invention, temperature-controlled engineered escherichia coli expressing GOX / LOX is combined with the photo-thermal hydrogel, a triple synergistic anti-tumor effect is triggered through 1064 nm laser, tumors are directly killed through photo-thermal heating, glucose / lactic acid is consumed in real time through GOX / LOX, energy metabolism is blocked, and a pentose phosphate pathway is inhibited; immunogenic cells are induced to die through a metabolite ROS, and whole-body anti-tumor immunity is activated.

The present invention discloses the application of a small molecule inhibitor of cuproptosis in myocardial ischemia-reperfusion injury, which relates to the technical field of myocardial ischemia-reperfusion injury. Specifically reducing or knocking out Cpeb3 expression can significantly promote myocardial repair, improve action potential stability, and reduce mortality. Therefore, Cpeb3 is a specific intervention target for cuproptosis in Gpc6<supgt;+< / supgt> CMs. The small molecule inhibitor of Cpeb3 can significantly reverse the changes in cuproptosis, aerobic respiration, and / or glycolysis triggered by Cpeb3 activation. In addition, the cuproptosis inhibitor can improve the survival rate of cardiomyocytes, reverse the changes in Cu-induced aerobic respiration and / or glycolysis, and reverse the changes in glycolysis, pentose phosphate pathway, and tricarboxylic acid cycle metabolites caused by Cu stimulation. Therefore, the present invention provides a new solution for the prevention and treatment of myocardial ischemia-reperfusion injury and has good application prospects.

The invention discloses a high-yield R-HPPA recombinant beauveria bassiana engineering bacterium, a construction method and application, an NADPH metabolic pathway is enhanced based on a multi-gene synergistic overexpression strategy, and efficient dynamic coupling of cofactor NADPH supply and hydroxylation reaction is realized by systematically regulating and controlling a phosphopentose pathway and a malic enzyme pathway, so that the yield of R-HPPA is increased, and the yield of R-HPPA is increased. According to the present invention, the beauveria bassiana hydroxylation reaction efficiency is improved, the efficient biosynthesis of the R-2-(4-hydroxyphenoxy) propionic acid is achieved, the fermentation process is optimized so as to promote the industrial application, and the new path is provided for breaking through the industrial technology bottleneck of the R-2-(4-hydroxyphenoxy) propionic acid biosynthesis.

The invention belongs to the technical field of traditional Chinese medicines and biological preparations, and discloses a Chinese yam composite peptide preparation, a preparation method and application of the Chinese yam composite peptide preparation in preparation of medicines for strengthening the spleen and the five internal organs. The Chinese yam composite peptide powder prepared by the invention can improve whole body diseases of rats with spleen deficiency type hyperlipemia through multi-target regulation and control of metabolic pathways, for example, up-regulation of a pentose phosphate pathway, promotion of glutathione metabolism, regulation of nerve-intestine-axis, synergistic restoration of visceral organ functions and the like, and the effect of'synergistic restoration of five internal organs' is achieved from'single spleen tonifying '. In addition, preventive intervention (a protection group) is coordinated with ROR alpha rhythm through early regulation of glutamine metabolism, so that compared with a treatment group, metabolic disorder is more remarkably reversed, multi-organ function repair is realized, body injury caused by hyperlipidemia is systematically improved, and finally, the effects of strengthening the spleen and strengthening the five internal organs are achieved, and the effect of'synergistic repair of the five internal organs' is achieved through regulation and control of metabolic roots.

The invention relates to the technical field of construction of recombinant bacteria, in particular to riboflavin-producing recombinant corynebacterium glutamicum as well as a construction method and application thereof. According to the construction method of the recombinant corynebacterium glutamicum for producing the riboflavin, a BsriDCAH gene is introduced, a pgi gene is weakened, and a carbon metabolic flow is reguided to a pentose phosphate pathway from a glycolysis pathway, so that the recombinant corynebacterium glutamicum for producing the riboflavin at high yield is obtained.

The invention provides a construction method and application of recombinant yarrowia lipolytica for synthesizing parthenolide. The construction method of the recombinant yarrowia lipolytica for synthesizing parthenolide comprises the following steps: overexpressing a parthenolide biosynthetic pathway optimized by codons in the yarrowia lipolytica; adaptive P450 enzyme reductase is screened, so that the electron transfer efficiency in a P450 system is improved; a heterologous non-oxidized phosphopentose pathway and an acetyl coenzyme A shuttle pathway are constructed to enhance the cytoplasmic acetyl-CoA level; the ATP-dependent transporter ABC-G1 is expressed to relieve the metabolic burden of the cells; the endoplasmic reticulum size regulation factor INO2 is expressed to restore the stable state of the endoplasmic reticulum, and the heme precursor ALA is added to improve the functional expression of P450 enzyme, so that the efficient synthesis of parthenolide is finally realized. The construction method of the yarrowia lipolytica for biosynthesis of parthenolide provided by the invention is simple to operate, and the constructed yarrowia lipolytica can efficiently produce parthenolide and has higher production and application values.

This invention provides a method for producing allicinol from recombinant *Escherichia coli* using glycerol and glucose as substrates. Using *E. coli* K-12 as the substrate host, a pathway for the synthesis of allicinol from glycerol and glucose is constructed by expressing genes fucA, yqaB, aldO, and rdh, and knocking out the glucose-specific phosphotransferase system IIBC component gene ptsG. Subsequently, genes mzwf, mgnd, alsE, and a6PP are introduced, while genes pfkA, gnd, and edd are knocked out to regulate the flux of the glycolysis and pentose phosphate pathways, thereby directing more carbon sources to the allicinol synthesis pathway. Finally, the formate dehydrogenase gene fdh and the glycerol kinase mutant gene glpK22 are introduced, and the methylglyoxal synthase gene mgsA is knocked out to construct a cofactor NADH cycle system and optimize the allicinol synthesis pathway, thus enabling *E. coli* to efficiently produce allicinol from glycerol and glucose through fermentation.