A kind of preparation method of cyclic adenosine monophosphate

A technology of cyclic adenosine monophosphate and tricarboxylic acid, applied in the direction of using bacteria, methods based on microorganisms, biochemical equipment and methods, etc., to achieve the effect of reducing the formation of organic acids

A technology of cyclic adenosine monophosphate and tricarboxylic acid, applied in the direction of using bacteria, methods based on microorganisms, biochemical equipment and methods, etc., to achieve the effect of reducing the formation of organic acids

CN102268469AInactive Publication Date: 2011-12-07NANJING UNIV OF TECH
2 Cites 12 Cited by

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Fermentation culture medium is in ordinary culture medium (based on the weight percent of culture medium, comprises glucose 5%, K 2 HPO 4 1%, KH 2 PO 4 1%, MgSO 4 1%, urea 0.5%, peptone 0.5%), add 0.1g NaF, 0.1g VB1 and 5g hypoxanthine per liter of medium.

[0026] Culture method: insert Arthrobacter A302 into the seed medium (based on the weight percentage of the medium, containing 1% glucose, 1% peptone, 0.5% yeast extract, 1% beef extract, and 0.3% NaCl), the initial pH is 7.0 , cultivated at 30Β° C. and 240 rpm for 18 hours. 10% of the inoculum was inserted into the fermentation medium in a 5L fermenter, the pH was controlled to be 7.0 with NaOH, the dissolved oxygen was controlled to be 30%, and fermentation was carried out at 400 rpm and 30Β° C. for 72 hours. When putting into tanks, the output of cyclic adenosine monophosphate is 10.2g / L, which is 3.25 times higher than the output (2.4g / L) when only using common culture medium.

Embodiment 2

[0028] The fermentation medium was added 6 g of hypoxanthine per liter of medium on the basis of ordinary medium (same as Example 1). The cultivation method is the same as in Example 1. When putting into tanks, the output of cyclic adenosine monophosphate is 4.6g / L, which is 0.92 times higher than the output (2.4g / L) when only using common culture medium.

Embodiment 3

[0030] The fermentation medium was added with 0.2 g of sodium fluoride per liter of medium on the basis of ordinary medium (same as in Example 1). The cultivation method is the same as in Example 1. When putting into tanks, the output of cyclic adenosine monophosphate is 3.2g / L, which is 0.33 times higher than the output (2.4g / L) when only using common culture medium.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention provides a method of preparing cyclic adenosine monophosphate, which includes the use of Arthrobacter sp. as an engineering bacterium, and the addition of precursors, inhibitors of glycolytic pathway and / or activators of tricarboxylic acid cycle into a fermentation medium, so as to acquire the cyclic adenosine monophosphate by fermentation. Further provided is a use of precursors, inhibitors of glycolytic pathway and / or activators of tricarboxylic acid cycle for preparing cyclic adenosine monophosphate by Arthrobacter sp.. The present invention inhibits the excessive glycolysis, increases the flux of pentose phosphate pathway, and enhances the tricarboxylic acid cycle by the addition of precursors, inhibitors of glycolytic pathway and / or activators of tricarboxylic acid cycle, so as to redistribute rationally the metabolic flux of central metabolic pathways, make the carbon flux more efficiently direct to the target product - cyclic adenosine monophosphate, and synthesize cyclic adenosine monophosphate by using carbon source efficiently.

Description

technical field [0001] The invention relates to a preparation method of cyclic adenosine monophosphate, in particular to a method for obtaining cyclic adenosine monophosphate with a high yield by using Arthrobacter as a production bacterium, adding regulating substances to a fermentation medium, and obtaining cyclic adenosine monophosphate with high yield through fermentation. Background technique [0002] Cyclic adenosine monophosphate (cAMP) is an important substance with physiological activity that widely exists in the human body. As a second messenger in cells, it plays an important role in the regulation of metabolism and synthesis of sugar, fat, nucleic acid, protein, etc. . It can be used clinically to treat angina pectoris, myocardial infarction, myocarditis and cardiogenic shock; it also has a certain effect on improving symptoms such as palpitations, shortness of breath and chest tightness of rheumatic heart disease; it can improve the curative effect of acute leuk...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
07 Dec 2011
Publication
CN102268469A
IPC
C12P19/32; C12R1/06
CPC
C12R1/06; C12P19/32; C12N1/205; C12R2001/06
Inventors
应汉杰; ζŸε»Ίζ–°