102 results about "Adenosine monophosphate.cyclic" patented technology

The present invention is directed to the use of a PDE4 inhibitor and a glucocorticoid to treat peripheral B-cell neoplasms. In particular, the present invention provides a method of treating individuals (e.g. patients) diagnosed with peripheral B-cell leukemias by administering pharmaceutical compositions comprising Type 4 cyclic adenosine monophosphate phosphodiesterase inhibitors and a glucocorticoid. Preferably, the combination of the PDE4 inhibitor and the glucocorticoid has a synergistic effect on apoptosis such that the level of apoptosis induced is greater than the level that would be expected by simply adding a PDE4 inhibitor to a glucocorticoid.

The invention discloses a method and application for inducing human umbilical cord mesenchyme stem cells to be differentiated into testicular interstitial cells. The method comprises the following step of culturing human umbilical cord mesenchyme stem cells of patients suffering from adenovirus and carrying mice steroidogenic factor-1 genes in a DMEM-F12 culture solution containing 0.3-3ng/ml of luteinizing hormone, 200-800mu M of dibutyryl cyclic adenosine monophosphate, 5*10<-6>-5*10<-4>M of all-trans retinoic acid (ATRA), 10mU/ml of human chorionic gonadotropin and 2.4uM of adrenocorticotrophic hormone for a week. Induced by the method in the invention, the human umbilical cord mesenchyme stem cells can be differentiated into testicular interstitial cells in vitro and provides important sources of cells for treating testosterone shortage by the cell replacing method or the genetic method.

The present invention relates to a technological process for extracting cyclophosphoadenosine from jujube. Said technological process includes the following steps: breaking, soaking, filtering soaking liquor, using ultrasonic technique to extract filter residue, combining extracts to obtain clear jujube juice; making the clear jujube juice be passed through ion exchange column, then making the eluent be again passed through ion exchange column, making the obtained secondary eluent be passed through chromatographic column, collecting solution containing cyclophosphoadenosine concentrating and crystallizing in ethyl alcohol so as to obtain high-purity cyclophosphoadenosine.

The invention relates to a method for extracting cyclic adenosine monophosphate (cAMP) from red jujubes, which comprises the steps of cleaning of the red jujubes, soaking of the red jujubes at normal temperature, precooking and pulping, zymohydrolysis on pectin mixed enzyme, filter pressing, anion resin combination, formic acid elution, nano-filtration and interception of formic acid with nano-filtration membrane, and purification, thus obtaining a finished product. The method solves the problems of complex current process, long period, low product purity and the like, and has the advantages of rich raw material sources, simple process, large processing amount, high extraction rate, high product purity and the like; the product is a natural non-toxic product; the finished product cAMP can be used for medical raw materials or used as food additive; the extracted byproduct red jujube juice can be continuously and deeply processed to promote the industrial chain of comprehensive development and achieve pollution-free production; the modern nano-filtration technology is applied to industrial production, therefore, not only the cost is reduced, but also a better path for deeply processing the red jujube raw material is provided, and the consumption of energy is greatly reduced; and the method not only has important economic benefit, but also has remarkable social benefits.

The invention provides a technique extracting cyclic adenosine monophosphate (cAMP) from Chinese dates. The Chinese dates are diacolated by water or ethanol solution or heated to extract; the extracting solution is connected to a resin column with macroporous adsorptive resins (OU-1 adsorptive resin) to absorb and is washed by the ethanol water or desorbed by acid water; the pH value of the stripping liquid is adjusted by sodium hydroxide and the stripping liquid is connected to the macroporous adsorptive resin column, and is desorbed by the ethanol with certain desorption after rinsing. The Chinese dates extractive product containing cAMP more than 1 per cent is gained after condensing and drying the stripping liquid. The invention is simple in technology, good in product quality, easy in preparation, high in extraction yield, free from three wastes and environment pollution, abundant in raw material source, low in cost and great in economic benefits.

The invention discloses a method for extracting cyclic adenosine monophosphate from dates, which comprises: pulverizing dates, adding water, mixing into pulp, microwaving the data pulp under the microwave power of 150-900 W for 10-450 seconds, microwaving the data pulp at 20-100 DEG C under the microwave power of 100-250 W for 10-60 minutes, carrying out the solid-liquid separation on the data pulp, and concentrating the separated liquid to obtain the concentrated cyclic adenosine monophosphate. The method enables all points in the data pulp to get the same action by utilizing the high-energyfunction of microwaves and the cavitation of the ultrasonic vibrations, so as to reduce the bonding force of the cyclic adenosine monophosphate and the data substrate, shorten the extraction time andincrease the extraction rate. In the extraction process, water is used as an extractant and no harmful chemical reagent for assistant extraction is added, so that the method can not cause the environmental pollution. Meanwhile, the method has the advantages of simple extraction technique and high extraction rate, and is suitable for industrial production.

The present invention provides a method of preparing cyclic adenosine monophosphate, which includes the use of Arthrobacter sp. as an engineering bacterium, and the addition of precursors, inhibitors of glycolytic pathway and/or activators of tricarboxylic acid cycle into a fermentation medium, so as to acquire the cyclic adenosine monophosphate by fermentation. Further provided is a use of precursors, inhibitors of glycolytic pathway and/or activators of tricarboxylic acid cycle for preparing cyclic adenosine monophosphate by Arthrobacter sp.. The present invention inhibits the excessive glycolysis, increases the flux of pentose phosphate pathway, and enhances the tricarboxylic acid cycle by the addition of precursors, inhibitors of glycolytic pathway and/or activators of tricarboxylic acid cycle, so as to redistribute rationally the metabolic flux of central metabolic pathways, make the carbon flux more efficiently direct to the target product - cyclic adenosine monophosphate, and synthesize cyclic adenosine monophosphate by using carbon source efficiently.

The invention discloses a preparation process of red date condensed juice high in cAMP content. The preparation process includes the following steps of firstly, conducting separating, washing and smashing; secondly, conducting hot water extraction; thirdly, conducting cooling and heat preservation enzymic method oriented conversion; fourthly, adding composite enzyme preparations; fifthly, inactivating enzymes; sixthly, conducting centrifugation; seventhly, conducting filtration; eighthly, conducting concentration and pasteurization; ninthly, conducting filling. Compared with the prior art, the preparation process has the advantages that the process is simple, the production period is shortened, and the energy loss is reduced; extraction is conducted after smashing is conducted, and therefore extraction of nutritional ingredients in red dates can be improved; by means of the extraction and enzymolysis combined method, the juice yield is greatly improved, the processing time is greatly shortened, and the cyclic adenosine monophosphate content in red date juice is improved; date peel is utilized, and the cAMP content is improved; preparations of pectinase, cellulose and protease are used in a composite mode, and the hydrolysis capacity of enzymic preparations is improved; the step-by-step enzymolysis and extraction process is adopted, and interference between related enzyme preparations is avoided.

The invention provides mixed type drink which takes Chinese yam and Chinese date as main raw materials, wherein Chinese date is rich in nutrient components such as protein, fat, sugar, carotene, vitamin B, vitamin C, vitamin P, calcium, phosphorus, Ferrum, cyclic adenosine monophosphate and the like; and Chinese yam contains amylase, polyphenol oxidase and other substances, is beneficial to spleen and stomach digestion and absorption function, also contains various nutrients, has functions of enhancing body and nourishing kidney and replenishing essence, is suitable for the weaker after illness, and improves human immune. The Chinese yam-Chinese date juice drink comprises the following raw materials in parts by weight: 180-220 parts of Chinese yam, 45-55 parts of Chinese date, 90-110 parts of xylitol, 1-3 parts of a stabilizer, 0.5-1.2 parts of an antioxidant and 630-700 parts of water. The drink prepared by the technical scheme has high nutrient components and good taste; and two raw materials are mixed to meet the need of health preservation for people and achieve mutual promotion and complementation.

The invention provides a method for fermentation production of cyclic adenosine monophosphate by controlling the dissolved oxygen content at two phases. The method adopts the two-phase dissolved oxygen content control manner to carry out batch fermentation in order to produce the cyclic adenosine monophosphate, fermentation strains are Arthrobacter sp having a preservation number of CGMCC No.3584, the dissolved oxygen in a thallus growth phase is 20-30%, and the dissolved oxygen is 5-10% after the thallus amount stabilizes. The method provided by the invention has the advantages of substantial improvement of the output of the cyclic adenosine monophosphate, no need of the addition of extra equipment in the fermentation process, saving of the electric energy for stirring and air supply in the later fermentation phase, and suitableness for industrialized production.

The invention relates to a method for extracting red date cyclic adenosine monophosphate through an ultrasonic composite membrane technology. The method solves the problem that the existing extraction technology has the defects of complex processes, low extraction rate, high industrialization difficulty and low product purity. The method comprises the following steps of removing date pits of cleaned red dates, crushing the red dates obtained by the previous step, adding an appropriate amount of an ethanol solvent into the crushed red dates, carrying out ultrasonic reinforcement extraction, repeatedly carrying out ultrasonic extraction of extracted filter residues, merging the extract obtained by the previous step, removing macromolecules of bacteria, proteins, pectin, polysaccharides and starch, and monosaccharides of glucose and fructose respectively through ultrafiltration and nanofiltration, intercepting cyclic adenosine monophosphate-rich concentrate, and carrying out pressure reduction condensation and spray drying to obtain 30% of a powdery cyclic adenosine monophosphate crude product. The method provided by the invention is easy for operation, is convenient for extraction, is fast, has short extraction time and a high cyclic adenosine monophosphate extraction rate, and is suitable for industrialized production. A product obtained by the method has high purity, is purely natural and does not produce chemical pollution. Therefore, the method provides an effective approach for comprehensive development of red date further processing.

The invention discloses a preparation method and application of a cigarette spina date seed extract. The method comprises the following steps: obtaining a mixed bacteria liquid by mixing a Raoultella planticola seed culture solution with a Bacillus methylotrophicus VJ4-1 seed culture solution; inoculating the mixed bacteria liquid to a mixed extract, and obtaining a fragmentation broth through fragmentation; and performing deposition pumping filtration on the fragmentation broth, performing pressure-reduction concentration on a filtrate, and removing a solvent so as to prepare the cigarette spina date seed extract with relative density of 1.20 to 1.36. According to the invention, the mixed extract composed of a spine date seed extract, a grifola frondosus extract and a lychee seed extract is taken as the only carbon source, a nitrogen source and a substrate, alkaloid, triterpenes, flavonoids, seventeen amino acids and the like are included, ferulic acid, vitamin C, plant sterol, cyclic adenosine monophosphate and the like are also included, compared to a single carbon source, a microbial enzyme system is more easily utilized for fermentation and fragrance production, the source is wide and easily available, and the method and application also have the advantages of naturalness, environmental protection and zero harm.

The invention discloses a crystallization method of cyclic adenosine monophosphate. The crystallization method comprises the following steps: adding an organic solvent used as an anti-solvent into 10-400g/L of a cyclic adenosine sodium phosphate aqueous solution at pH of 7.0-9.0 under the condition of stirring, carrying out pumping filtration after crystallization is completely finished, washing crystals by the use of ethanol, and carrying out vacuum drying to obtain cyclic adenosine sodium phosphate crystals; dissolving the above cyclic adenosine sodium phosphate crystals in water to obtain a saturated solution of the cyclic adenosine sodium phosphate crystals, adjusting pH of the solution to 4.0-5.5 by the use of acid while stirring, carrying out crystal growing for 0.5-8h, adjusting pH to 1.0-2.5, carrying out pumping filtration after crystallization is completely finished, washing crystals by the use of ethanol, and carrying out vacuum drying to obtain cyclic adenosine monophosphate crystals. By the adoption of the method provided by the invention, quality of the final product is obviously improved, yield of the crystal product is stably raised, and granular size of the crystals can be controlled by changing crystallization stirring speed or temperature. The method provided by the invention is simple to operate, has good repeatability, and is suitable for industrial production of cyclic adenosine sodium phosphate.

The invention discloses a method for extracting cyclic adenosine monophosphate and application of cyclic adenosine monophosphate, and belongs to the technical field of extraction of biological active substances. The method provided by the invention comprises the following steps: adjusting the pH of a fermentation liquor by using an alkaline liquor; performing centrifugal separation to obtain a supernatant; concentrating the supernatant, and performing first discoloration; fractionally precipitating a discolored solution by using an organic solvent; dissolving an obtained precipitate, and performing second discoloration; performing micro porous filtration, and concentrating a second discolored solution; adding the organic solvent for precipitating the concentrated second discolored solution; and performing centrifugal separation, and drying the precipitate in vacuum to obtain a finished product. By adopting the method provided by the invention, the conventional ionic exchange and resin chromatography processes can be replaced, and the waste water amount can be lowered by 82.5 percent; meanwhile, the production period is shortened greatly, and the production time is shortened by 36 percent; and the prepared cyclic adenosine monophosphate is stable in quality, can be over 97 percent in purity, and can be widely applied to the fields of medicine, foods, feeds and the like.

The invention discloses compound nourishing wine and a making method thereof. The compound nourishing wine comprises a nutrient extracting solution, wherein the nutrient extracting solution comprises a black date extracting solution prepared by performing solid fermentation on red dates, a black Chinese wolfberry fruit extracting solution, a black bean extracting solution, a black sesame extracting solution and a black garlic extracting solution; black dates obtained by performing solid fermentation on the red dates are characterized in that cane sugar content is lowered, fructose and glucose contents are increased, and fructo-oligosaccharide content is increased; total acid is increased, and mainly oxalic acid and citric acid are increased; flavonoid content and polyphenol content are high; cyclic adenosine monophosphate is abundant, and important physiological activity is achieved; moreover, the black date extracting solution obtained by performing solid fermentation on the red dates is obtained through treatment under an ultrahigh pressure of 100 to 150Mpa for 20 to 30 seconds; compared with an untreated black date, the treated black date has the advantages that the juice yield is increased by 10 to 15 percent, the active substance extracting rate is increased by 30 to 40 percent, and the effect of the nourishing wine is brought into play.

The invention discloses red date healthcare wine which is made of the following raw materials in parts by weight: 45-55 parts of red dates, 15-25 parts of sorghum, 0.1-0.3 part of lycium barbarum, 0.1-0.2 part of astragalus membranaceus, 0.03-0.06 part of donkey-hide gelatin, 1-3 parts of pearl barley and 1-3 parts of small red beans. The making method comprises the following steps: firstly, mixing the sorghum, the pearl barley and the small red beans, boiling, and fermenting so as to obtain a sorghum mixture; putting boiled water into crushed red date granules, further uniformly mixing with lycium barbarum, astragalus membranaceus and donkey-hide gelatin, cooling, fermenting, distilling, sealing, and leaving to stand at a cold and dry place with good air ventilation, thereby obtaining thered date healthcare wine. The red date healthcare wine contains phenolic substances, vitamins, carotene, cyclic adenosine monophosphate, iron and the like, and has the effects of clearing lung and clearing heat, enriching blood and generating muscle, preventing aging, reducing blood pressure and promoting digestion.

A production process of cyclic adenosine monophosphate is disclosed. The production process is characterized by comprising: a step 1) of filtering by utilization of a ceramic membrane to remove impurities, namely a step of filtering a cyclic adenosine monophosphate fermentation broth by a ceramic-membrane filtering device to leave hyphae, suspended matters, colloid and macromolecule organic compounds in filter residue so as to obtain a fermentation broth dialyzate; a step 2) of performing continuous fluid separation, namely a step of feeding the fermentation broth dialyzate into a continuous fluid separation system, allowing effective components to be adsorbed by resin and inactive components to be separated, and resolving and eluting the resin by using an eluant to obtain a cyclic adenosine monophosphate crude product; a step 3) of desalination and concentration, namely a step of subjecting the crude product to desalination and concentration to obtain a high-purity high-concentration cyclic adenosine monophosphate solution; and a step 4) of concentration and drying, namely a step of subjecting the cyclic adenosine monophosphate solution to vacuum concentration and drying to obtain the high-purity cyclic adenosine monophosphate. The production process is advantaged by reducing the cost, increasing the yield, improving product quality, and achieving continuous production.

The invention provides a preparation method of a jujube extract. The jujube extract is obtained by the processes of cleaning jujubes, pre-cooking, pulping, and performing ultrasonic breaking, enzymolysis, biological fermentation, flash-evaporation concentration and biological fermentation. According to the preparation method of the jujube extract, provided by the invention, active ingredients in jujubes are not extracted, separated and purified, and jujube essence can be efficiently prepared by using a biological fermentation technology, an enzyme technology, an ultrasonic-assisted crushing technology, a flash evaporation concentration technology and a spray-drying technology, thereby having the efficacies of jujube polysaccharide, flavone, saponin, cyclic adenosine monophosphate and other functional factors; meanwhile, the technical difficulty in active substance separation, preparation and research is solved.

The invention discloses a method for preparing cyclic adenosine monophosphate by adenylate cyclase. The method comprises the following steps that the adenylate cyclase serves as a catalyst, adenosinetriphosphate serves as a substrate, and in the presence of Mg <2+>, a reaction solution containing the cyclic adenosine monophosphate is obtained. According to the adenylate cyclase, an amino acid sequence as shown in SEQ ID NO.14 is provided, the stability is good, the half-life period is long at medium and low temperatures, ATP can be efficiently catalyzed to generate cAMP in one step at mediumtemperature, a substrate conversion rate reaches 90 % or above, the advantages of simple industry, mild conditions, short period, few by-products and the like are achieved, the clean and pollution-free effects are achieved, and the energy conservation, consumption reduction and emission reduction in the cAMP production process can be realized.