Technique for extracting cycli phosphate adenosine from chinese date

A technology of cyclic adenosine monophosphate and a process method, which is applied in the field of extracting cyclic adenosine monophosphate from jujube, can solve the problems of loss of target components, production cost, increase of process steps, difficulty in industrialization, etc., and achieve abundant raw materials and good product quality , no effect of the three wastes

Inactive Publication Date: 2008-01-23
天津欧瑞生物科技有限公司
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

As can be seen from the above, the current method for isolating cyclic adenosine monophosphate from jujube is very cumbersome and difficult to industrialize
Simultaneously, the increase of process steps will inevitably cause t

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: after getting 10g jujube pulverized, extract with 20% ethanol aqueous solution 25 ℃. The extract was cooled to room temperature and then filtered to obtain a transparent crude extract. 10 mL of macroporous resin adsorption resin OU-1 resin (manufactured by Tianjin Ou Rui Biotechnology Co., Ltd., Q / 12XQ0365-2005) was installed in an exchange column with an inner diameter of 1.5 cm. Then rinse with 40ml of deionized water to remove impurities; then rinse with 50ml of 0.5mol / L hydrochloric acid aqueous solution, and adjust the pH of the rinse solution to 4.5 with 8% sodium hydroxide to obtain cAMP desorption solution 1. Then the desorption solution 1 is passed into an exchange column with an internal diameter of 1.5cm that is equipped with 20mL of macroporous adsorption resin ME-2 (produced by Tianjin Orui Biotechnology Co., Ltd., Q / 12XQ0362-2005), washed with 10ml of water, and rinsed with 40ml of 20% ethanol. The aqueous solution was desorbed to obtain a d...

Embodiment 2

[0019] Embodiment 2: After taking 10 g of jujube and pulverizing it, extract it with an aqueous solution at 40°C. The extract was cooled to room temperature and then filtered to obtain a transparent crude extract. Put 10mL of OU-1 resin in an exchange column with an inner diameter of 1.5cm, put 100mL of jujube crude extract on the column, discard the effluent, and then rinse with 60ml of deionized water to remove impurities; then use 70ml of 0.8mol / L The hydrochloric acid aqueous solution was rinsed, and the pH of the eluent was adjusted to 4.5 by 8% sodium hydroxide to obtain cAMP desorption solution 1. Then the desorption solution 1 is passed into an exchange column with a built-in macroporous adsorption resin ME-2 resin 20mL internal diameter of 1.5cm, washed with 10ml of water, and desorbed with 40ml of 20% ethanol aqueous solution to obtain the desorption solution 2, which is concentrated and dried to obtain the desorption solution 2. Jujube extract containing cAMP, dete...

Embodiment 3

[0020] Embodiment 3: After getting 500g jujube and pulverizing, extract with 20% ethanol aqueous solution 50 ℃. The extract was cooled to room temperature and then filtered to obtain a transparent crude extract. Put 500mL of OU-1 resin in an exchange column with an inner diameter of 5cm, put 5000mL of jujube crude extract on the column, discard the effluent, and then rinse with 200ml of deionized water to remove impurities; then use 300ml of 1.0mol / L hydrochloric acid The aqueous solution was rinsed, and the pH of the eluate was adjusted to 4.5 by 8% sodium hydroxide to obtain cAMP desorption solution 1. Then the desorption solution 1 is passed into the exchange column with a built-in macroporous adsorption resin ME-2 resin 1000ml internal diameter of 5cm, washed with 1000ml of water, and desorbed with 2000ml20% ethanol aqueous solution to obtain the desorption solution 2, which is concentrated and dried to obtain the desorption solution 2. Jujube extract of cAMP, determined ...

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PUM

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Abstract

The invention provides a technique extracting cyclic adenosine monophosphate (cAMP) from Chinese dates. The Chinese dates are diacolated by water or ethanol solution or heated to extract; the extracting solution is connected to a resin column with macroporous adsorptive resins (OU-1 adsorptive resin) to absorb and is washed by the ethanol water or desorbed by acid water; the pH value of the stripping liquid is adjusted by sodium hydroxide and the stripping liquid is connected to the macroporous adsorptive resin column, and is desorbed by the ethanol with certain desorption after rinsing. The Chinese dates extractive product containing cAMP more than 1 per cent is gained after condensing and drying the stripping liquid. The invention is simple in technology, good in product quality, easy in preparation, high in extraction yield, free from three wastes and environment pollution, abundant in raw material source, low in cost and great in economic benefits.

Description

technical field [0001] The invention relates to the application of a macroporous adsorption resin in the separation and purification of traditional Chinese medicine components, in particular to a process for extracting cyclic adenosine monophosphate (cAMP) from jujube. Background technique [0002] my country is the country with the most abundant jujube resources in the world, and it is also the only country in the world that exports red dates. Jujube is the ripe fruit of the jujube plant of the Rhamnaceae family (Rhamnaceae). Jujube is also known as jujube, which originates in China. The unique production and cultivation advantages of Chinese jujube resources in the world have huge development space and economic benefits. Cyclic nucleotides are important biologically active substances with the most prominent characteristics in jujube fruits. They are protein kinase activators, an important physiologically active substance that exists widely in organisms, and intermediary f...

Claims

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Application Information

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IPC IPC(8): C07H19/213A61K36/725
Inventor 欧来良王为超张华孔德领
Owner 天津欧瑞生物科技有限公司
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