Production process of cyclic adenosine monophosphate

Abstract

A production process of cyclic adenosine monophosphate is disclosed. The production process is characterized by comprising: a step 1) of filtering by utilization of a ceramic membrane to remove impurities, namely a step of filtering a cyclic adenosine monophosphate fermentation broth by a ceramic-membrane filtering device to leave hyphae, suspended matters, colloid and macromolecule organic compounds in filter residue so as to obtain a fermentation broth dialyzate; a step 2) of performing continuous fluid separation, namely a step of feeding the fermentation broth dialyzate into a continuous fluid separation system, allowing effective components to be adsorbed by resin and inactive components to be separated, and resolving and eluting the resin by using an eluant to obtain a cyclic adenosine monophosphate crude product; a step 3) of desalination and concentration, namely a step of subjecting the crude product to desalination and concentration to obtain a high-purity high-concentration cyclic adenosine monophosphate solution; and a step 4) of concentration and drying, namely a step of subjecting the cyclic adenosine monophosphate solution to vacuum concentration and drying to obtain the high-purity cyclic adenosine monophosphate. The production process is advantaged by reducing the cost, increasing the yield, improving product quality, and achieving continuous production.

Description

technical field [0001] The invention relates to a production field for preparing cyclic adenosine monophosphate, in particular to a production process of cyclic adenosine monophosphate. Background technique [0002] Cyclic adenosine monophosphate (cAMP for short) is one of the important contents of current molecular biology research and has the function of a second messenger. It is a protein kinase activator, which is a derivative of nucleotides. It is an important substance with physiological activity that exists widely in the human body. It is produced by adenosine triphosphate under the catalysis of adenylyl cyclase, and can regulate various cellular functions. functional activity. As the second messenger of hormones, hormones play a role in regulating physiological functions and substance metabolism in cells, can change the function of cell membranes, and promote calcium ions in the reticulum sarcoplasm to enter muscle fibers, thereby enhancing myocardial contraction an...

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of large solvent loss, low yield, high cost, small output and serious environmental pollution when using a separation column in the prior art for the separation and purification of cyclic adenosine monophosphate. Provide an improved production of cyclic adenosine monophosphate, in order to achieve the purpose of reducing production costs, shortening the production cycle, and improving product purity and yield

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