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Preparation method and application of cigarette spina date seed extract

The technology for extracting sour jujube kernels and sour jujube kernels is applied in the field of tobacco flavors, and can solve the problems of single aroma taste, insufficient aroma intensity, complicated extraction steps, etc. Creamy sweet effect

Inactive Publication Date: 2017-04-05
HUBEI CHINA TOBACCO IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the defects that the extraction steps of chemically synthesized tobacco extract are complicated, the environment is seriously polluted, the aroma and taste of the tobacco extract is single and the aroma intensity is still insufficient, the present invention provides a preparation method and application of the extract of Chinese jujube seed for tobacco

Method used

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  • Preparation method and application of cigarette spina date seed extract
  • Preparation method and application of cigarette spina date seed extract

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Experimental program
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Effect test

Embodiment 1

[0034] The preparation method of Suanzaoren extract 1 for tobacco comprises the following steps:

[0035] 1) Reflux extraction of Semen jujube Semen and water at a mass ratio of 1:20, then filter for 3 hours, and sterilize the filtrate at a high temperature of 115°C for 25 minutes to obtain Semen Semanthus Semen Semen Jujube Extract;

[0036] 2) Weighing 80 parts of Jujube Seed Extract, 20 parts of Grifola frondosa Extract and 10 parts of Litchi Kernel Extract by weight and parts, and mix them uniformly to obtain a mixed extract;

[0037] 3) Strain activation: inoculate Raoultella planticola VP4-4, preservation number CCTCCNO: M 2012005, on the slant medium of Raoultella planticola, and obtain the activated strain after static culture at 38°C for 24 hours. Place in a 4°C refrigerator; wherein, the slant culture medium of Raoultella phytogenes is: wort: 120mL, sucrose: 20g / L, agar: 15g / L.

[0038]4) Preparation of Raoultella phyticum seed culture solution: inoculate the activa...

Embodiment 2

[0045] The preparation method of Suanzaoren extract 2 for tobacco comprises the following steps:

[0046] 1) Reflux extraction of Semen jujube Semen and water at a mass ratio of 1:30, then filter for 2 hours, and sterilize the filtrate at 115° C. for 25 minutes; the extract of Semen Semanthus Semen Semen Semanae is obtained;

[0047] 2) Weighing 60 parts of Jujube Seed Extract, 30 parts of Grifola frondosa Extract and 10 parts of Litchi Kernel Extract by weight and parts, and mix them uniformly to obtain a mixed extract;

[0048] 3) Strain activation: Inoculate Raoultella planticola VP4-4, preservation number CCTCCNO: M 2012005, on the slant medium of Raoultella planticola, and culture it statically at 30°C for 72 hours to obtain the activated strain. Place in a refrigerator at 4°C; among them, the Raoultella vegetative slant medium is: wort: 120mL, sucrose: 20g / L, agar: 15g / L;

[0049] 4) Preparation of Raoultella vegetative seed culture solution: Inoculate the activated str...

Embodiment 3

[0056] The preparation method of Suanzaoren extract 3 for tobacco comprises the following steps:

[0057] 1) Reflux extraction of Semen jujube Semen and water at a mass ratio of 1:40, then filter for 1 hour, and sterilize the filtrate at a high temperature of 115°C for 25 minutes; the extract of Semen Semanthus Semen Semen Semanthus Semen is obtained;

[0058] 2) Weighing 70 parts of Jujube Seed Extract, 35 parts of Grifola frondosa Extract and 15 parts of Litchi Kernel Extract by weight and parts, and mixed uniformly to obtain a mixed extract;

[0059] 3) Activation of strains: Inoculate Raoultella planticola VP4-4, preservation number CCTCCNO:M 2012005, on the slant medium, and culture at 25°C for 24 hours to obtain activated strains, and place them in the refrigerator; Wherein, the slant medium is: wort juice: 120mL, sucrose: 20g / L, agar: 15g / L.

[0060] 4) Preparation of Raoultella vegetative seed culture solution: Inoculate the activated strain obtained in step 3) into t...

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Abstract

The invention discloses a preparation method and application of a cigarette spina date seed extract. The method comprises the following steps: obtaining a mixed bacteria liquid by mixing a Raoultella planticola seed culture solution with a Bacillus methylotrophicus VJ4-1 seed culture solution; inoculating the mixed bacteria liquid to a mixed extract, and obtaining a fragmentation broth through fragmentation; and performing deposition pumping filtration on the fragmentation broth, performing pressure-reduction concentration on a filtrate, and removing a solvent so as to prepare the cigarette spina date seed extract with relative density of 1.20 to 1.36. According to the invention, the mixed extract composed of a spine date seed extract, a grifola frondosus extract and a lychee seed extract is taken as the only carbon source, a nitrogen source and a substrate, alkaloid, triterpenes, flavonoids, seventeen amino acids and the like are included, ferulic acid, vitamin C, plant sterol, cyclic adenosine monophosphate and the like are also included, compared to a single carbon source, a microbial enzyme system is more easily utilized for fermentation and fragrance production, the source is wide and easily available, and the method and application also have the advantages of naturalness, environmental protection and zero harm.

Description

technical field [0001] The invention relates to the technical field of spices for tobacco, in particular to a preparation method and application of jujube seed extract for tobacco. Background technique [0002] In tobacco products, the aroma and taste of tobacco are very important. Adding spices is a key link in the cigarette process, and it is also an effective means to improve the product style and improve the quality of cigarette aroma. Tobacco flavors can be divided into chemical synthetic flavors and natural flavors. In recent years, the research and development of natural plant flavors has been the focus and hotspot of ingredients because of their Chinese characteristics, natural harmlessness, and easy availability of raw materials. Applying microbial fermentation engineering to the biosynthesis of flavors and fragrances, using Chinese herbal medicine plants as raw materials, various natural flavors can be produced by using microorganisms, which is a good solution to ...

Claims

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Application Information

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IPC IPC(8): A24B15/26A24B3/12C11B9/00C11B9/02
CPCA24B15/26A24B3/12C11B9/00C11B9/02
Inventor 魏敏罗诚浩宋旭艳陈义坤岳海波
Owner HUBEI CHINA TOBACCO IND
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