Arthrobacter for fermentative production of cyclic adenosine monophosphate and application thereof

A technology of cyclic adenosine monophosphate and Arthrobacter, applied in the field of microorganisms, can solve the problems limited to the laboratory stage, and achieve the effects of high yield, stable traits, light damage, and wide mutation spectrum

Active Publication Date: 2011-12-07
NANJING BIOTOGETHER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic method can efficiently catalyze adenosine triphosphate to generate cyclic adenosine monophosphate, but this method is only limited to the laboratory stage, and there is still a certain distance from industrialization
[0004] At present, there is no domestic report on the preparation of cyclic adenosine monophosphate by fermentation

Method used

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  • Arthrobacter for fermentative production of cyclic adenosine monophosphate and application thereof
  • Arthrobacter for fermentative production of cyclic adenosine monophosphate and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Taking the cyclic adenosine monophosphate-producing strain isolated from the soil as the starting strain, the N + Ion beam mutagenesis (the vacuum degree in the target chamber of the low-energy ion implanter is 2×10 -4 , the injection energy is 20kev, and the dose is 5×10 13 ions cm -2 .s -1 ) after the spore suspension was diluted 100 times with sterile water, got 0.1ml and spread it on the beef extract plate medium and cultivated it for 3 days at 30°C, from which a single colony was selected and cultivated on the beef extract slant for 2 days. Transfer one loop to a 500mL shake flask containing 30mL liquid seed medium (glucose 10g / L, peptone 10g / L, yeast extract 5g / L, beef extract 10g / L, NaCl 3g / L), at 30°C Incubate on a shaker at 250rpm for 20 hours. Then transfer 4mL to 40mL liquid fermentation medium (glucose 50g / L, K 2 HPO 4 10g / L, KH 2 PO 4 10g / L, MgSO 4 1g / L, urea 5g / L, peptone 5g / L) in a 500mL shake flask, 280rpm shaker fermentation culture at 30°C for...

Embodiment 2

[0033] Transfer the bacterial strain A302 from the beef extract slant to a 500mL shaker containing 30mL liquid seed medium (glucose 20g / L, peptone 8g / L, yeast extract 5g / L, beef extract 15g / L, NaCl 3g / L). In the bottle, cultured on a shaker at 250 rpm at 30°C for 20 hours. Transfer 4mL to 40mL liquid fermentation medium (glucose 40g / L, K 2 HPO 4 10g / L, KH 2 PO 4 5g / L, MgSO 4 4g / L, urea 8g / L, peptone 5g / L) in a 500mL shake flask, 260rpm shaker fermentation culture at 32°C for 72 hours. The yield of cyclic phosphate reached 2.0g / L.

Embodiment 3

[0035] Transfer the bacterial strain A302 from the beef extract slant to a 500mL shaker containing 30mL liquid seed medium (glucose 10g / L, peptone 3g / L, yeast extract 4g / L, beef extract 10g / L, NaCl 3g / L). In the bottle, cultured on a shaker at 250 rpm at 30°C for 20 hours. Transfer 4mL to 40mL liquid fermentation medium (glucose 45g / L, K 2 HPO 4 5g / L, KH 2 PO 4 10g / L, MgSO 4 0.5g / L, urea 3g / L, peptone 5g / L) in the 500mL shaking flask, under 30 ℃ 300rpm shaker fermentation culture 72 hours. The yield of cyclic phosphate reached 3.6g / L.

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Abstract

The invention provides arthrobacter for producing cyclic adenosine monophosphate through fermentation and application thereof. With a preservation number of CGMCC No.3584, the arthrobacter of the invention has a A302 strain which is three times higher than an original strain in terms of the cyclic adenosine monophosphate output. Undergoing over 10 generations of passage, the A302 strain of the invention is stable in producing cyclic adenosine monophosphate.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a high-yield cyclic adenosine monophosphate Arthrobacter and its application. Background technique [0002] Cyclic adenosine monophosphate is an important substance with physiological activity that widely exists in the human body. As a second messenger in cells, it plays an important role in the regulation of metabolism and synthesis of sugar, fat, nucleic acid, protein, etc. It is clinically used to treat angina pectoris, myocardial infarction, myocarditis and cardiogenic shock; it also has a certain effect on improving symptoms such as palpitations, shortness of breath, and chest tightness of rheumatic heart disease; it can improve the curative effect of acute leukemia combined with chemotherapy, and can also be used for acute leukemia Induction remission; in addition, it also has certain curative effect on elderly chronic bronchitis, various hepatitis and psoriasis. Cycl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/32C12R1/06
CPCC12P19/40C12R1/06C12N1/205C12R2001/06
Inventor 应汉杰柏建新陈晓春熊健陈勇
Owner NANJING BIOTOGETHER
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