Technological process for extracting cyclic adenosine monophosphate (CAMP) from red dates

Abstract

The invention provides a technological process for extracting cyclic adenosine monophosphate (CAMP) from red dates. The technological process sequentially comprises the following steps: 1, soaking and pulping the red dates, extracting with hot water at the temperature of 65-75 DEG C for 3-5 times, enzymolyzing with pectinase, lixiviating and filter pressing to obtain date juice; 2, mixing the date juice with anion exchange resin in a stirring tank and stirring for specific adsorption, and obtaining the anion exchange resin combined with the CAMP in the date juice; 3, filling the combined resin into an upper chromatographic column, and eluting with methanoic acid solution to obtain methanoic acid eluent; 4, distilling out methanoic acid from the methanoic acid eluent by a rotary evaporator or obtaining the methanoic acid through membrane separation, wherein, the obtained intercept substance contains the CAMP; and 5, purifying the intercept substance by a silicagel column chromatography purification technology, and finally obtaining the high-purity CAMP. The CAMP extracted from the red dates by the process has the advantages of high purity, high production efficiency, low production cost and no environmental pollution, and is applicable to industrial production.

Description

Technical field: [0001] The invention belongs to a process for extracting food extracts, in particular to a process for extracting cyclic adenosine monophosphate from jujube. Background technique: [0002] The cultivated area of ​​jujube in my country exceeds 10 million mu, accounting for 99% of the total cultivated area in the world. Jujube is a fruit with both medicine and food, which is rich in cyclic adenosine monophosphate (CAMP). Cyclic adenosine monophosphate (CAMP) and cyclic guanosine monophosphate (CGMP) are intermediary factors for cell transmission of hormones and transmitters. They are the second messengers of intracellular hormone activities and participate in the metabolism of sugar, fat, protein and other substances. Cyclic adenosine monophosphate (CAMP) has the effect of inhibiting the growth of cancer cells and turning cancer cells into normal cells. It can be used for the treatment of angina pectoris of coronary heart disease and cardiogenic shock of myoc...

AI Technical Summary

Problems solved by technology

[0005] At present, the process of extracting cyclic adenosine monophosphate (CAMP) in foreign laboratories has the following defects: 1. Dowex I-type resin from the United States is used, and the resin is 200-400 mesh only for laboratory research, and the price is expensive

2. To extract cAMP, the amount of formic acid eluent is small, and after NH 4 OH is neutralized, not recycled, direct discharge is wasteful and causes environmental pollution

3. Use thin-layer chromatography to purify cAMP. This method requires scraping off the cAMP zone on the silica gel plate and then separating cAMP. Not only is the operation cumbersome, but it is also not suitable for industrial production.

4. Because the extraction of cAMP is limited to laboratory research, the amount of formic acid eluent is small, and no membrane separation technology has been used