A method for separating cyclic adenosine monophosphate

A technology of cyclic adenosine monophosphate and anion exchange column, which is applied in the field of separation of cyclic adenosine monophosphate, can solve the problems of small adsorption capacity, poor separation and purification effect, etc., achieve less resin usage, high product recovery rate, and elution process simple effect

Active Publication Date: 2011-12-07
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the purpose of the present invention is to overcome the problems of small adsorption capacity and poor separation and purification effect of the method for separating cyclic adenosine using cation exchange resins in the prior art, and to provide a method for separating cyclic adenosine with large adsorption capacity and good separation and purification effect. Adenosine method

Method used

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  • A method for separating cyclic adenosine monophosphate
  • A method for separating cyclic adenosine monophosphate

Examples

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Embodiment 1

[0023] This example is used to illustrate the method for separating cyclic adenosine monophosphate provided by the present invention.

[0024] Fill the fixed bed with 30g of anion exchange resin (Amberlite IRA95), equilibrate and put the cyclic adenosine monophosphate supernatant liquid with a concentration of 5g / L on the column. After 500mL, the adsorption is saturated, and then the impurities are washed with 1mol / L ammonia water (detected by HPLC It is determined that there is no impurity flowing out), after the washing is completed, it is eluted with 0.05mol / L HCl, the volume of the eluent is 898mL, the concentration is 2.71g / L, the purity is 95.1%, and the yield is 97.4%.

Embodiment 2

[0026] This example is used to illustrate the method for separating cyclic adenosine monophosphate provided by the present invention.

[0027] Use 50g of anion exchange resin (Amberlite IRA93) to fill the fixed bed, put the cyclic adenosine monophosphate supernatant liquid with a concentration of 5.5g / L on the column after equilibrium, absorb and saturate after 791mL, and then wash impurities with 0.004mol / L ammonia water (with HPLC detection determines that there is no impurity to flow out), after washing the impurities, then use 0.2mol / L HCl to elute, the volume of the eluent is 1.563L, the concentration is 2.71g / L, the purity is 94.3%, and the yield is 97.4% %.

Embodiment 3

[0029] This example is used to illustrate the method for separating cyclic adenosine monophosphate provided by the present invention.

[0030]Fill the fixed bed with 100g of anion-exchange resin (Amberlite IRA95), and put the cyclic adenosine monophosphate supernatant liquid with a concentration of 4.89g / L on the column after equilibrium, after 1.820L, the adsorption is saturated, and then use 0.001mol / L sodium hydroxide aqueous solution To wash impurities, after the washing is completed, 0.05mol / L HCl is used for elution, and the volume of the eluent is 3.249L. The eluate was synchronously concentrated and desalted with a nanofiltration membrane with a molecular weight cut-off of 150 Daltons, crystallized and dried to obtain cyclic adenosine monophosphate with a purity of 95.2% and a yield of 96.6%.

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Abstract

The invention relates to a method for separating cyclic adenosine monophosphate, which comprises: absorbing solution of cyclic adenosine monophosphate by anion exchange column; purifying the anion exchange column absorbing the solution of cyclic adenosine monophosphate with alkaline solution serving as a purifying agent; and eluting the anion exchange column by using an acid solution as an eluting agent. The method provided by the invention separates 3',5'-cyclic adenosine monophosphate by using a novel anionite; and compared with the conventional method which separates 3',5'-cyclic adenosine monophosphate by using cation exchange resin, the method provided by the invention has the advantages of high absorption capacity, simple subsequent eluting process and high product yield. When the method is used for the industrial production of 3',5'-cyclic adenosine monophosphate, the separation and purification processes are simple, the production period is short, and the consumption resin is small.

Description

technical field [0001] The invention relates to a method for separating cyclic adenosine monophosphate. Background technique [0002] Cyclic adenosine monophosphate, also known as 3′, 5′-cyclic adenosine monophosphate (3′, 5′-cyclic adenosine monophosphate, referred to as cAMP), is a protein kinase activator and a derivative of nucleotides. It is an important substance with physiological activity that widely exists in the human body. It is produced by adenosine triphosphate under the catalysis of adenylyl cyclase and can regulate various functional activities of cells. As the second messenger of hormones, hormones play a role in regulating physiological functions and substance metabolism in cells, can change the function of cell membranes, and promote calcium ions in the reticulum sarcoplasm to enter muscle fibers, thereby enhancing myocardial contraction and promoting the respiratory chain Oxidase activity can improve myocardial hypoxia, relieve symptoms of coronary heart ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H19/213C07H1/06C07H1/08
Inventor 应汉杰钱文斌陈晓春柏建新陈勇熊健林晓清
Owner NANJING UNIV OF TECH
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