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High-yield DHA schizochytrium limacinum strain as well as culture method and application thereof

A technology of Schizochytrium and a culture method, applied in the field of microorganisms, can solve the problems of low DHA fermentation yield and the like, and achieve the effects of high fermentation yield, high DHA content, and increased yield

Active Publication Date: 2015-01-28
SHANDONG BAILONG CHUANGYUAN BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the deficiency of the low yield of the existing DHA fermentation, and provides a high-yield DHA strain of Schizochytrium and its cultivation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: Mutation breeding of Schizochytrium BLCY-01

[0036] The wild strain of Schizochytrium sp. BLCY-01 of the present invention is isolated from the Yellow Sea waters of Qingdao City, Shandong Province. The seawater sterilized by filter membranes in advance is placed in the culture medium that has been sterilized, and pine pollen is used to make The bait is cultured at 20-30°C for 2-3 days, streaked on the plate with pollen and cultivated until colonies are formed, picked a single colony and streaked repeatedly until a pure culture with consistent cell shape is obtained, and inoculated in test tubes and glycerol tubes for storage. A wild strain of Schizochytrium sp. BLCY-01 was obtained.

[0037] The components per liter of screening medium are as follows:

[0038] Glucose 20g, yeast extract 5g, peptone 5g, sea crystal 15g, penicillin 200mg, streptomycin 200mg.

[0039] The wild strain of Schizochytrium sp. BLCY-01 was mutagenized and selected by means of ul...

Embodiment 2

[0057] The culture method of above-mentioned Schizochytrium sp. (Schizochytrium sp.) BLCY-01, the steps are as follows:

[0058] (1) Inoculate Schizochytrium sp. BLCY-01 in the activation medium, activate and culture at 20-30°C for 24-48 hours to obtain the activated strain;

[0059] (2) Take the activated bacterial strain obtained in step (1), inoculate it in the seed liquid medium, and cultivate it for 24-48 hours at 20-30° C. and 200-240 r / min to obtain Schizochytrium sp. .) BLCY-01 bacterial liquid.

[0060] The components per liter of the activation medium are as follows:

[0061] Glucose 30g, peptone 10g, yeast extract powder 5g, sea crystal 15g, water to 1L, pH natural.

[0062] The components per liter of the seed liquid culture medium are as follows:

[0063] Glucose 30g, peptone 10g, yeast extract powder 5g, sea crystal 15g, dipotassium hydrogen phosphate 1.5g, anhydrous magnesium sulfate 0.5g, soybean oil 0.3g, water to 1L, pH natural.

Embodiment 3

[0064] Example 3: Effects of different carbon source concentrations on the biomass and DHA oil content of Schizochytrium BLCY-01:

[0065] Configure the medium according to the different carbon source concentrations shown in Table 2, each 500ml Erlenmeyer flask contains 100ml of medium, inoculate 5ml (v / v) of the seed solution into it respectively, shake and cultivate at 27°C and 220rpm for 54h, measure Schizochytrium biomass and DHA oil content. The results are shown in Table 2.

[0066] Table 2: Effects of different carbon source concentrations on the biomass and DHA oil content of Schizochytrium BLCY-01

[0067] Glucose (g / L)

[0068] 40

[0069] As can be seen from Table 2, when the initial glucose concentration was 30g / L, the bacterial biomass was the highest; as the glucose concentration increased, the biomass gradually decreased, which may be caused by the glucose effect; while the DHA content was 90g / L L is the highest, at this time the growth of ...

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Abstract

The invention relates to a high-yield DHA schizochytrium limacinum strain as well as a culture method and an application thereof. Schizochytrium limacinum (Schizochytrium sp.) BLCY is reserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms on July, 15, 2014, wherein the address is China Academy of Sciences Institute of Microbiology, #3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No:9461. The invention further relates to a culture method and an application of the strain. By optimizing the components of the culture medium and the culture conditions, the biomass of the schizochytrium limacinum after being fermented for 54-72 hours reaches 30-45 g / L, the DHA content reaches 8.6-12.5 g / L, which is higher than the fermentation yields of microorganisms reported in the existing patents and literatures.

Description

technical field [0001] The invention relates to a high-yielding DHA-producing Schizochytrium strain, a culture method and application thereof, and belongs to the technical field of microorganisms. Background technique [0002] With the development and evolution of modern living habits and dietary culture, people's awareness and requirements for food health care are also increasing, and the awareness of the importance of polyunsaturated fatty acids (PUFAs) to human health has reached a fever pitch. And docosahexaenoic acid (DHA), the molecular formula is C 22 h 32 o 2 , is favored as an important member of the omega-3 series of polyunsaturated fatty acids (PUFA). Studies have shown that: DHA contains a special structure of five unsaturated bonds, so it has important physiological functions for human health. For example: regulate blood lipids, regulate the immune system, prevent and treat cardiovascular diseases, cancer and other functions; in addition, DHA is also the mai...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12N1/14C12P7/6427C12N1/145C12R2001/645
Inventor 黎丽窦光朋干昭波霍文严魏巍
Owner SHANDONG BAILONG CHUANGYUAN BIO TECH
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