Preparation method of schizochytrium limacinum powder

A technology of Schizochytrium powder and Schizochytrium, which is applied in the field of microorganisms, can solve the problems of inability to maintain the integrity of the cell structure and poor rumen protection effect, and achieve the effects of increasing DHA content, avoiding damage, and improving absorption rate

Active Publication Date: 2020-04-17
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The wall-breaking method selected by this invention is sand grinding and homogenization, which will completely rupture the cells and form cell fragments, and the oil will be released, which cannot maintain the complete structure of the cells. In addition, the rumen-passing protective agents selected by the invention such as shellac and acrylic resin No. Ⅱ, acrylic resin No. Ⅲ, polyvinyl alcohol acetate phthalate, hydroxypropyl methylcellulose phthalate, Opadry or sargassum gel are enteric-coated coating agents, which are easily dissolved in the rumen environment Or decompose, the rumen protection effect is not good

Method used

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  • Preparation method of schizochytrium limacinum powder
  • Preparation method of schizochytrium limacinum powder
  • Preparation method of schizochytrium limacinum powder

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preparation example Construction

[0041] A preparation method of Schizochytrium powder, characterized in that it comprises the following steps,

[0042] Embedding: mix Schizochytrium cells with wall materials, emulsifiers, and antioxidants, stir, shear, and homogenize to obtain a suspension;

[0043] Drying: drying the obtained suspension to obtain Schizochytrium powder with a water content of less than 5%;

[0044] Coating: transfer the obtained dry powder into a coating machine, and spray the coating solution prepared by the coating agent at 30-40°C to obtain Schizochytrium algae powder;

[0045] The Schizochytrium thallus is obtained after the fermentation liquid of the Schizochytrium strain is subjected to acid treatment, alkali treatment or bio-enzyme treatment for wall-breaking pretreatment.

[0046] Further, the Schizochytrium sp.CGMCC No.6843 or Schizochytrium sp.ATCC No.20888 or Schizochytrium sp.ATCC No.20889 or Schizochytrium sp.ATCC No.28209 or Schizochytrium limacinum Honda et YokochiATCC MYA-138...

Embodiment 1

[0065] Embodiment 1: DHA fermented liquid preparation

[0066] The slant-preserved strain of Schizochytrium sp. CGMCC No.6843 was inserted into a 2L shake flask containing 400 mL of seed medium, and cultured at a temperature of 25° C. at a speed of 200 rpm for 24 hours to complete the activation culture of the strain. According to the inoculum amount of 0.5%, the shake flask seed liquid is connected to the first-level seed fermenter equipped with the sterilized seed medium, and the cultivation temperature is 28°C, the ventilation volume is 1vvm, and the stirring speed is 50rpm, and the cultivation is carried out for 30h, and the first-level seed expansion cultivation is completed. . According to the inoculation amount of 5%, the seed liquid of the first-level seed tank is connected to the second-level seed fermentation tank equipped with sterilized seed medium, and the culture temperature is 28 ℃, the ventilation rate is 1vvm, and the stirring speed is 75rpm. Cultivate for 24h...

Embodiment 2

[0069] Embodiment 2: the preparation of Schizochytrium powder A

[0070] Get embodiment 1 gained DHA fermented liquid 10m 3 , add caustic soda to adjust the pH value to 10.0, raise the temperature to 50°C, stir for 60 min, then add hydrochloric acid to adjust the pH value to neutral. Then add starch sodium octenyl succinate 50kg, whey protein 50kg, monoglyceride 50kg, butyl hydroxyanisole 0.5kg, propyl gallate 0.25kg, vitamin E 0.25kg, stir at 40°C for 30min, 2900r / Min cutting for 10 minutes, after obtaining a stable suspension, go to spray drying, the spray drying inlet air temperature is 160-180°C, and the outlet air temperature is 70-80°C, and the dried bacteria powder is obtained.

[0071] Add 150kg of polyacrylic acid resin No. IV and 50kg of ethyl cellulose into 1000L of ethanol, stir and dissolve evenly to obtain a coating solution. The above-mentioned dried bacteria powder is moved into a coating machine, sprayed into the coating solution, and the temperature when t...

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Abstract

The invention discloses a preparation method of schizochytrium limacinum powder. The preparation method comprises the following steps: mixing schizochytrium limacinum thalli with a wall material, an emulsifier and an antioxidant, stirring, shearing and homogenizing to obtain a suspension; drying the obtained suspension to obtain schizochytrium limacinum powder of which the water content is less than 5%; transferring the obtained dry powder into a coating machine, and spraying a coating solution prepared from a coating agent at 30-40 DEG C to obtain schizochytrium limacinum powder; wherein theschizochytrium limacinum thallus is obtained by performing acid treatment or alkali treatment or wall-breaking pretreatment of biological enzyme treatment on fermentation liquor after schizochytrium limacinum strain fermentation. The prepared schizochytrium limacinum powder is used for feeding animals, can be prevented from being degraded in rumens of the animals, and can be absorbed by the animals more easily after passing through the rumens, so that milk of the animals is rich in DHA.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a preparation method of Schizochytrium powder. Background technique [0002] Schizochytrium algae powder is the algae powder obtained by fermenting and culturing the Schizochytrium fungus and drying the fermented liquid. Because it is rich in DHA and other polyunsaturated fatty acid oils, adding it to animal feed can greatly promote the growth of animals. [0003] DHA, the English abbreviation of DocosaHexaenoic Acid (DocosaHexaenoic Acid), commonly known as "brain gold", is a very important polyunsaturated fatty acid for the human body, and plays a vital role in the nervous system and visual development of babies , It also plays an important role in the heart, brain, eyes, and immune system of adults. [0004] DHA has been widely used in dairy products such as infant formula milk powder and children's milk. At present, DHA fortification is mostly achieved by adding DHA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/10A23K10/12A23K10/14A23K40/30A23K20/147A23K20/158A23K20/10A23K20/111A23K20/174A23K10/30A23K20/163
CPCA23K50/10A23K10/12A23K10/14A23K40/30A23K20/147A23K20/158A23K20/10A23K20/111A23K20/174A23K10/30A23K20/163
Inventor 吴轶王炳荣徐鲁明廖炜程韩雯王跃飞
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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