Method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid
A docosahexaenoic acid and chemical accelerator technology, applied in the biological field, can solve problems such as unclear metabolic mechanism and limited development and application
Active Publication Date: 2015-06-24
KUNMING ZAONENG BIOTECH CO LTD
View PDF1 Cites 3 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
On the premise of not inhibiting the growth of Cryptidinosa, it is an important direction to improve the fermentation culture by adding various substances that stimulate the accumulation of Cryptidinium oil in the medium, but its metabolic mechanism is not clear, which limits its research. development and application of
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0018] The composition of a heterotrophic medium for Cryptidinium kourii is: glucose 9 g / L, yeast extract powder 2 g / L, sea salt 25 g / L. In said heterotrophic medium, the plant growth regulator was indole acetic acid (IAA) at a concentration of 2.5 mg / L. After culturing at 25 oC for 72 hours, the oil was extracted, and the DHA content of the culture was measured by GC-MS. At the same time, cell metabolites were extracted from parallel cultures, and the changes in intracellular metabolites were detected and analyzed by LC-MS.
Embodiment 2
[0020] The situation is basically the same as that of Example 1, except that the plant growth regulator added therein is naphthaleneoxyacetic acid (BNOA) at a concentration of 4 mg / L.
Embodiment 3
[0022] The situation of Example 1 is basically the same, except that the plant growth regulator added therein is chlorobenzoic acid, and the concentration is 4 mg / L.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more PUM
Login to view more Abstract
The invention provides a method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid (DHA). The method comprises the following steps: with a heterotrophic culture medium of crypthecodinium cohnii as a foundation, adding any chemical promoter into the heterotrophic culture medium; the heterotrophic culture medium is composed of 9g/L of glucose, 2g/L of yeast extract powder and 25g/L of sea salt; the chemical promoter can also be called a plant growth regulator, including indoleacetic acid (IAA), naphthoxyessigsaeure (BNOA), chlorobenzoic acid, salicylic acid (SA), abscisic acid (ABA) and cholamine, and the concentrations of these chemical promoters in the heterotrophic culture medium are as follows: 2.5mg/L of indoleacetic acid (IAA), 4mg/L of naphthoxyessigsaeure (BNOA), 4mg/L of chlorobenzoic acid, 1mg/L of salicylic acid (SA), 20mg/L of abscisic acid (ABA) and 2.5mM cholamine; culturing at 25 DEG C for 72 hours, extracting grease, and measuring the DHA content of the culture by using a gas chromatograph-mass spectrometer, meanwhile, extracting cellular metabolite from a parallel culture, and detecting and analyzing the content change condition of the cellular metabolite by using a metabonomics method.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a method for improving the fermentative production of DHA by heterotrophic microorganisms by using various chemical accelerators. Background technique [0002] DHA is docosahexaenoic acid (Docosahexaenoic Acid, DHA) is one of the important substances for human brain development and growth, and is an important component of the brain and retina. The largest proportion, accounting for about 50%. Therefore, it plays an important role in the development of the brain and retina of fetuses and infants, as well as the maintenance of adult brain functions. In addition, DHA also has a positive effect on diseases of the cardiovascular system such as coronary heart disease, stroke, hypertension, nervous system diseases such as neurological disorders, Alzheimer's disease and depression. As an essential fatty acid, DHA has more significant effects on enhancing memory and thinking ability and imp...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more Application Information
Patent Timeline
Login to view more Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6427
Inventor 张卫文陈磊刘璐
Owner KUNMING ZAONENG BIOTECH CO LTD
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Try Eureka
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap