Method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid
A docosahexaenoic acid and chemical accelerator technology, applied in the biological field, can solve problems such as unclear metabolic mechanism and limited development and application
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Embodiment 1
[0018] The composition of a heterotrophic medium for Cryptidinium kourii is: glucose 9 g / L, yeast extract powder 2 g / L, sea salt 25 g / L. In said heterotrophic medium, the plant growth regulator was indole acetic acid (IAA) at a concentration of 2.5 mg / L. After culturing at 25 oC for 72 hours, the oil was extracted, and the DHA content of the culture was measured by GC-MS. At the same time, cell metabolites were extracted from parallel cultures, and the changes in intracellular metabolites were detected and analyzed by LC-MS.
Embodiment 2
[0020] The situation is basically the same as that of Example 1, except that the plant growth regulator added therein is naphthaleneoxyacetic acid (BNOA) at a concentration of 4 mg / L.
Embodiment 3
[0022] The situation of Example 1 is basically the same, except that the plant growth regulator added therein is chlorobenzoic acid, and the concentration is 4 mg / L.
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