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Method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid

A docosahexaenoic acid and chemical accelerator technology, applied in the biological field, can solve problems such as unclear metabolic mechanism and limited development and application

Active Publication Date: 2015-06-24
KUNMING ZAONENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the premise of not inhibiting the growth of Cryptidinosa, it is an important direction to improve the fermentation culture by adding various substances that stimulate the accumulation of Cryptidinium oil in the medium, but its metabolic mechanism is not clear, which limits its research. development and application of

Method used

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  • Method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid
  • Method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid
  • Method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid

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Experimental program
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Embodiment 1

[0018] The composition of a heterotrophic medium for Cryptidinium kourii is: glucose 9 g / L, yeast extract powder 2 g / L, sea salt 25 g / L. In said heterotrophic medium, the plant growth regulator was indole acetic acid (IAA) at a concentration of 2.5 mg / L. After culturing at 25 oC for 72 hours, the oil was extracted, and the DHA content of the culture was measured by GC-MS. At the same time, cell metabolites were extracted from parallel cultures, and the changes in intracellular metabolites were detected and analyzed by LC-MS.

Embodiment 2

[0020] The situation is basically the same as that of Example 1, except that the plant growth regulator added therein is naphthaleneoxyacetic acid (BNOA) at a concentration of 4 mg / L.

Embodiment 3

[0022] The situation of Example 1 is basically the same, except that the plant growth regulator added therein is chlorobenzoic acid, and the concentration is 4 mg / L.

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Abstract

The invention provides a method for improving heterotrophic microbial fermentation via multiple chemical promoters to produce docosahexenoic acid (DHA). The method comprises the following steps: with a heterotrophic culture medium of crypthecodinium cohnii as a foundation, adding any chemical promoter into the heterotrophic culture medium; the heterotrophic culture medium is composed of 9g / L of glucose, 2g / L of yeast extract powder and 25g / L of sea salt; the chemical promoter can also be called a plant growth regulator, including indoleacetic acid (IAA), naphthoxyessigsaeure (BNOA), chlorobenzoic acid, salicylic acid (SA), abscisic acid (ABA) and cholamine, and the concentrations of these chemical promoters in the heterotrophic culture medium are as follows: 2.5mg / L of indoleacetic acid (IAA), 4mg / L of naphthoxyessigsaeure (BNOA), 4mg / L of chlorobenzoic acid, 1mg / L of salicylic acid (SA), 20mg / L of abscisic acid (ABA) and 2.5mM cholamine; culturing at 25 DEG C for 72 hours, extracting grease, and measuring the DHA content of the culture by using a gas chromatograph-mass spectrometer, meanwhile, extracting cellular metabolite from a parallel culture, and detecting and analyzing the content change condition of the cellular metabolite by using a metabonomics method.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for improving the fermentative production of DHA by heterotrophic microorganisms by using various chemical accelerators. Background technique [0002] DHA is docosahexaenoic acid (Docosahexaenoic Acid, DHA) is one of the important substances for human brain development and growth, and is an important component of the brain and retina. The largest proportion, accounting for about 50%. Therefore, it plays an important role in the development of the brain and retina of fetuses and infants, as well as the maintenance of adult brain functions. In addition, DHA also has a positive effect on diseases of the cardiovascular system such as coronary heart disease, stroke, hypertension, nervous system diseases such as neurological disorders, Alzheimer's disease and depression. As an essential fatty acid, DHA has more significant effects on enhancing memory and thinking ability and imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6427
Inventor 张卫文陈磊刘璐
Owner KUNMING ZAONENG BIOTECH CO LTD
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