A method for ultralow-temperature storage and renewal cultivation of wild rice stem tips includes steps: taking a subcultured sterile tissue culture seedling, keeping the base portion, culturing in a culture medium free of hormone MS, cutting new sprouts of the base portion, transferring to a culture medium containing 0.3molL<-1> of sucrose and 0.5-5mgL<-1> of abscisic acid to realize culturing, transferring to a culture medium containing 0.7molL<-1> of sucrose and 0.5-5mgL<-1> of abscisic acid to realize preculture, stripping the stem tip, pre-culturing in an MS solid culture medium containing 0.4molL<-1> of sucrose and 2molL<-1> of glycerin, gently dipping in 2-4% calcium-free sodium alginate solution, putting on a loading strip, immersing in an MS liquid culture medium containing 0.1-0.3% of calcium chloride, 0.4molL<-1> of sucrose and 2molL<-1> of glycerin, immobilizing, sucking excessive liquid, treating in a pretreatment solution, transferring to a vitrification protective agent PVS2 to realize ice-bath treatment, and putting into liquid nitrogen to realize storage.The method has the advantages of simplicity, convenience and feasibility in storage, stability, reliability and favorable recovery growth conditions of stored wild rice.