Preparation method of new natural abscisic acid
A technology of natural abscisic acid and seeds, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of glucose repression, high production cost, low fermentation yield, etc., so as to reduce production cost and improve acid production. rate effect
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Embodiment 1
[0171] Use 25 1000ml Erlenmeyer flasks, each with 300ml medium A, sterilize at 120°C, inoculate the activated abscisic acid high-yielding strain Botrytis cinerea TBC-10CGMCC No.1889 spore suspension after cooling, place at 25°C, Shake flasks were incubated for 24-46 hours. Inoculate the cultured primary seed liquid into a 100 liter fermenter (secondary seed tank) with 50 liters of medium B inside by 13%-15% inoculum, and cultivate it with aeration and stirring at a temperature of 26°C-28°C for 24 hours. -40 hours.
[0172] Use a 1-ton fermenter for the third-stage fermentation. The medium C in the tank is about 500L. After sterilizing with conventional high-pressure hot steam sterilization, inoculate the seed liquid of the second-stage tank with 10%-15% inoculum, and ferment with aeration and stirring for 20 - After 40 hours, feed medium C is fed intermittently, the intermittent time is to feed once every 10-15 hours, and the amount of each feed is 0.1%-0.5% of the total volu...
Embodiment 2
[0177] Use 50 1000ml Erlenmeyer flasks, each with 300ml medium A, sterilize at 120°C, inoculate the activated abscisic acid high-yielding strain Botrytis cinerea TBC-10 CGMCC No.1889 spore suspension after cooling, and place at 25°C , shake flasks for 24-33 hours. Inoculate the cultured primary seed liquid into a 200 liter fermenter (secondary seed tank) with 100 liters of medium B inside by 15%-18% inoculum, and cultivate it with aeration and stirring at a temperature of about 26°C for 24-30 Hour.
[0178] Use a 1-ton fermenter for the third-stage fermentation. The medium C in the tank is about 500L. After sterilizing with conventional high-pressure hot steam sterilization, inoculate the seed liquid of the second-stage tank with 20%-25% inoculum, and ferment with aeration and stirring for 15 - After 24 hours, feed medium C is fed intermittently, the intermittent time is 1-3 times every 20-28 hours, and the amount of each feed is 0.05%-0.3% of the total volume of the fermenta...
Embodiment 3
[0183] Use 30 1000ml Erlenmeyer flasks, each containing 300ml medium A, sterilize at 120°C, inoculate the activated abscisic acid high-yielding strain Botrytis cinerea TBI-9 CGMCC No.0500 spore suspension after cooling, place at 27°C, Shake flasks were incubated for 24-33 hours. Inoculate the cultured primary seed liquid into a 100 liter fermenter (secondary seed tank) with 50 liters of medium B inside by 15%-20% inoculation amount, and cultivate it with aeration and stirring at a temperature of about 26°C for 24-30 Hour.
[0184] Use a 1-ton fermenter for the third-stage fermentation. The medium C in the tank is about 500L. After sterilizing with conventional high-pressure hot steam sterilization, inoculate the seed liquid of the second-stage tank with 10%-15% of the inoculation amount, and ferment with aeration and stirring for 15 - After 24 hours, continuously feed medium C at a rate of 0.1-5 L / h until 10 hours before stopping fermentation (lower tank). Add inositol conti...
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