Hybrid rice pre-harvest sprouting inhibitor, preparation method thereof and method for inhibiting hybrid rice pre-harvest sprouting
A technology for hybrid rice and inhibitors, applied in the field of hybrid rice panicle germination inhibitors and its preparation, to suppress hybrid rice panicle germination, to achieve the effect of eliminating slight germination inhibition, good safety and environmental protection benefits, and extensive resources
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Embodiment 1
[0031] 1. Test material: hybrid rice Qianyou 0508 F 1 seed;
[0032] 2. Preparation of medicaments: prepare the three medicaments into required concentrations according to Table 1, and obtain panicle germination inhibitors A, B, and C respectively;
[0033] 3. Spraying time and method: During the period from the end of milk ripening to the beginning of yellow ripening, spray evenly according to the dosage of 45kg per mu (add a little Tween 20 to the anti-ear germination agent, which is beneficial to adhere to the ears of rice), and spray as a control Equal amount of water.
[0034] Creation of the ear bud environment: the next day after spraying, spray water every 30 minutes from 6:00 am to 6:00 pm every day, and spray water for 5 minutes each time until the leaves drip to keep the ear layer moist. The water spraying lasts for 7-10 days to ensure that the relative humidity in the field is more than 85% during the maturation of rice.
[0035] Sampling method: Sampling is don...
Embodiment 2
[0063] 1. Test material: hybrid rice Qianyou 0508 F 1 seed.
[0064] 2, test agent: panicle germination inhibitor C (with embodiment 1), and single agent D (40mg / kg abscisic acid), single agent E (160mM cyclohexylamine), single agent F (60mg / kg eugenol) And drug G (single agent D and single agent E are mixed in equal volume), agent H (single agent D and single agent F are mixed in equal volume), agent I (single agent E and single agent F are mixed in equal volume).
[0065] 3. Test method: The test was carried out at the Zijingang Campus Farm of Zhejiang University in 2019, and sowed in stages according to the growth period characteristics of the parents to ensure that the parents meet at the flowering period. Three replicate plots were set up for each treatment, with an area of 2.4m 2 .
[0066] Drug preparation: using water as a solvent, prepare panicle germination inhibitor C, single agent D, single agent E, single agent F, agent G, agent H, and agent I.
[0067] Spra...
Embodiment 3
[0083] 1. Test material: hybrid rice Qianyou 0508 F 1 seed.
[0084] 2, test medicament: In the embodiment 1, panicle germination inhibitor C, panicle germination inhibitor J (eugenol concentration 400mg / kg, the concentration of uniconazole is 600mg / kg, and the concentration of maleic hydrazide is 400mg / kg) .
[0085] 3. Test method:
[0086] Drug preparation and seed treatment: rice seeds were sterilized by 0.1% sodium hypochlorite solution and sprayed evenly with clean water (CK) and the test drug respectively, and the seeds were taken out and dried after one hour.
[0087] Germination conditions and index determination: with embodiment 1.
[0088] Statistical analysis: with embodiment 1.
[0089] 4. Test results: The indoor seed soaking test of the panicle germination inhibitor C of the present invention and the existing panicle germination inhibitor J showed that the panicle germination inhibitor C can significantly prolong the germination time of Qianyou 0508 seeds an...
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