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Method for synchronously separating and measuring endogenous abscisic acid, gibberellins and auxin in turfgrass

A technology of synchronous separation and abscisic acid, applied in material separation, measuring devices, and analysis of materials, etc., can solve the problem of long time consumption, low recovery rate, complicated detection operation of plant endogenous hormones, and failure to meet the needs of rapid detection of turfgrass endogenous hormones and other problems, to achieve the effect of high recovery rate, cost saving and high detection sensitivity

Inactive Publication Date: 2014-05-28
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology

[0007] The purpose of the present invention is mainly to provide a method for synchronous separation and determination of turfgrass endogenous abscisic acid for the detection of plant endogenous hormones, which is complex in operation, time-consuming and low in recovery rate, and cannot meet the needs of rapid detection in the detection of turfgrass endogenous hormones. , gibberellin and auxin methods

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  • Method for synchronously separating and measuring endogenous abscisic acid, gibberellins and auxin in turfgrass
  • Method for synchronously separating and measuring endogenous abscisic acid, gibberellins and auxin in turfgrass

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Embodiment Construction

[0023] Below in conjunction with embodiment describe in detail:

[0024] 1. Test material: Bermudagrass material treated with salt stress (treatment concentration: 150 mmol / L NaCl) for 30 days (selected as C136 Bermudagrass from the Lawn Germplasm Resource Garden of Wuhan Botanical Garden, Chinese Academy of Sciences).

[0025] 2. Detection steps

[0026] 1) Extraction of endogenous hormones

[0027] A. Extraction: Take 0.5g fresh sample, grind it with liquid nitrogen, then add 5 ml of extraction solution, and extract overnight at -20°C to obtain the extraction solution;

[0028] The extract is methanol: formic acid: water = 15:1:4, according to v / v, plus 40 mg / ml 2,6-di-tert-butyl-4-methylphenol as an antioxidant;

[0029] B. Centrifugation: Centrifuge the extract at 15,000 rpm and 4°C for 10 minutes, collect the supernatant and store it in a new tube at -20°C, extract the residue with 5ml of extract at -20°C for 1 hour, and centrifuge. Collect the supernatant and mix it w...

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Abstract

The invention discloses a method for synchronously separating and measuring an endogenous abscisic acid, gibberellins and auxin in turfgrass, and relates to the technical field of plant endogenous hormone analysis. The method provided by the invention comprises the steps as follows: (1) fast extracting the three major hormones namely, the endogenous abscisic acid, the gibberellins and the auxin; (2) solid phase extracting and eluting; (3) conducting high efficient liquid chromatogram-mass spectrometry. According to the method provided by the invention, the pretreatment is easy and consumes less time, the consumption of a chemical reagent is little, the cost is saved effectively, the detection is high in sensitivity, good in selectivity and requires no derivatization, and as a separation extraction method, a chromatograph separation condition and a mass spectrometric detection method enable such three major hormones as the endogenous abscisic acid, the gibberellins and the auxin to be measured directly and synchronously in the crude extract of a turfgrass sample, the method has a high recovery rate reaching 94.6-96.5%.

Description

technical field [0001] The invention relates to the field of determination of plant endogenous hormones, in particular to a method for synchronously separating and measuring endogenous abscisic acid, gibberellin and auxin of lawn grass. Background technique [0002] With the development of my country's economy and the strengthening of ecological environment awareness, as well as the acceleration of urbanization and the improvement of urban greening standards, the development of the turf industry has ushered in a good opportunity, and the demand for turfgrass continues to increase. As an important part of the urban ecosystem, the lawn can purify the air, sterilize, reduce noise, prevent soil erosion and improve the microclimate in the prevention and control of pollution and the improvement of the ecological environment, and has a strong ornamental and recreational value. . In today's frequent smog, how to improve our living environment has a long way to go, and turfgrass pla...

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Application Information

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IPC IPC(8): G01N30/02
Inventor 傅金民胡龙兴娄燕宏
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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