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Laying hen daily ration capable of improving quality of eggs

A laying hen and seed technology, applied in the field of laying hen diets, can solve problems such as limited fish resources, damage to platelets, and human hazards, and achieve the effects of wide application, fast deposition speed, and enhanced memory

Inactive Publication Date: 2014-08-27
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many problems in fish oil and vegetable raw materials (linseed, flax oil, etc.) as the source of producing DHA eggs: fish resources are limited, it is impossible to use fish to produce fish oil without restriction; polyunsaturated fatty acids in fish oil It is easy to oxidize, and it must be prepared and used immediately when adding it. The oxidized products of EPA and DHA contained in fish oil not only make eggs have a fishy smell, but also have great harm to the human body; the origin of fish oil and the preparation process of fish oil will affect The quality of fish oil makes it difficult to control the quality of fish oil, which ultimately affects the quality of eggs
At present, the content of DHA in Thraustochytrium produced by conventional fermentation is not high, and the oil contains a lot of EPA. EPA will affect the growth and development of fetuses, children and adolescents, and destroy platelets, making blood difficult to coagulate and other side effects.

Method used

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  • Laying hen daily ration capable of improving quality of eggs
  • Laying hen daily ration capable of improving quality of eggs
  • Laying hen daily ration capable of improving quality of eggs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Fermentation process 1 of embodiment 1 Thraustochytrium

[0021] 1. Medium

[0022] 1.1 Seed medium (per L)

[0023] Glucose 20g, yeast powder 2g, NaCl25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g, NaHCO 3 0.1g, vitamin B 1 0.01mg, vitamin B 12 0.001 mg.

[0024] 1.2 Fermentation medium (per L)

[0025] Tapioca starch 80g, yeast powder 15g, NaCl 25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g,NaHCO 3 0.1g, vitamin B 1 0.012mg, vitamin B 12 0.0012mg, biotin 0.05mg, initial pH 6.5.

[0026] 2. Training method

[0027] 2.1 Seed culture: Culture in a shaker flask, take a Thraustochytrium slant, add 7ml of sterile water, scrape off the spores with a flat spatula, inoculate them in the seed medium after shaking evenly, at 28°C, 170r / min Under activation for 48h.

[0028] 2.2 Fermentation culture: Under the protection of a fire ring, inoculate the seeds in a 500L fermenter with 300L ferm...

Embodiment 2

[0031] The fermentation process 2 of embodiment 2 Thraustochytrium

[0032] 1. Medium

[0033] 1.1 Seed medium (per L)

[0034] Glucose 15g, yeast powder 2g, NaCl10g, MgSO 4 ·7H 2 O4g, KCl0.5g, KH 2 PO 4 0.05g, CaCO 3 0.15g, (NH 4 ) 2 SO 4 0.15g, NaHCO 3 0.1g, Vitamin B 1 0.005mg, vitamin B 12 0.003mg.

[0035] 1.2 Fermentation medium (per L)

[0036] Tapioca starch 50g, yeast powder 10g, NaCl 20g, MgSO 4 ·7H 2 O4g, KCl, 0.5g, KH 2 PO 4 0.05g, CaCO 3 0.1g, (NH 4 ) 2 SO 4 0.1g,NaHCO 3 0.05g, vitamin B 1 0.02mg, vitamin B 12 0.002mg, biotin 0.1mg, initial pH 6.0.

[0037] 2. Training method

[0038] 2.1 Seed culture: Culture in a shaker flask, take a Thraustochytrium slant, add 7ml of sterile water, scrape off the spores with a flat spatula, inoculate them in the seed medium after shaking evenly, at 25°C, 180r / min Under activation for 60h.

[0039] 2.2 Fermentation culture: Under the protection of a fire ring, inoculate the seeds in a 500L fermenter with...

Embodiment 3

[0042] Fermentation process 3 of embodiment 3 Thraustochytrium

[0043] 1. Medium

[0044] 1.1 Seed medium (per L)

[0045] Glucose 20g, yeast powder 2g, NaCl25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g, NaHCO 3 0.1g, vitamin B 1 0.01mg, vitamin B 12 0.001mg.

[0046] 1.2 Fermentation medium (per L)

[0047] Tapioca starch 80g, yeast powder 15g, NaCl 25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g,NaHCO 3 0.1g, vitamin B 1 0.012mg, vitamin B 12 0.0012mg, biotin 0.05mg, initial pH 6.5.

[0048] 2. Training method

[0049] 2.1 Seed culture: Culture in a shaker flask, take a Thraustochytrium slant, add 7ml of sterile water, scrape off the spores with a flat spatula, inoculate them in the seed medium after shaking evenly, at 28°C, 170r / min Under activation for 48h.

[0050] 2.2 Fermentation culture: Under the protection of a fire ring, inoculate the seeds in a 500L fermenter with 300L ferme...

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Abstract

The invention discloses a laying hen daily ration capable of improving quality of eggs. The laying hen daily ration contains thraustochytrium powder. A method for fermenting the thraustochytrium comprises the following steps; (1) culturing seeds, namely, inoculating thraustochytrium spores in a seed culture medium to activate; (2) fermenting to culture, namely, inoculating the activated seeds into a fermentation tank for fermentation and culture, after being cultured for 36-60 hours, adding 1-4% cocinic acid into the fermentation tank in a uniform speed so as to increase the DHA (Docosahexaenoic Acid) content, wherein the temperature within 84-108 hours in the front culture period is 22-25 DEG C, and the temperature in the later period is 28-30 DEG C for inhibiting generation of EPA (Timnodonic Acid). Due to addition of the dry thraustochytrium powder into the daily ration, the DHA content of eggs is directly increased, the DHA deposition velocity is fast, the time for enriching and stabilizing the DHA in eggs is shortened, and the eggs are free of EPA and thus are applicable to more people.

Description

technical field [0001] The invention relates to a diet for laying hens that can improve the quality of eggs, in particular to a diet for laying hens containing dry powder of Thraustochytrium fungus. Background technique [0002] DHA (Docosahexaenoic acid, namely docosahexaenoic acid) is an omega-3 long-chain polyunsaturated fatty acid (omega-3PUFA). Its health effects on the human body have been widely studied and proven, including anti-inflammatory, reducing The occurrence of cardiovascular diseases such as atherosclerosis, inhibiting tumor growth, and contributing to fetal growth and development. In recent years, DHA-enhanced products have been continuously developed and entered the fields of biopharmaceuticals and nutritional health products. At present, they are mainly extracted from deep-sea fish oil or algae oil to form capsule preparations or powder preparations through microencapsulation technology. By adding raw materials rich in unsaturated fatty acids in poultry ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K1/00A23K1/16A23K1/18C12R1/645A23K10/18A23K50/75
Inventor 班甲李浩洋陈骏佳朱文浩钟志才徐晓燕曹惠华班雯婷王帆周正雄张卫东
Owner GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
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