Laying hen daily ration capable of improving quality of eggs
A laying hen and seed technology, applied in the field of laying hen diets, can solve problems such as limited fish resources, damage to platelets, and human hazards, and achieve the effects of wide application, fast deposition speed, and enhanced memory
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Embodiment 1
[0020] Fermentation process 1 of embodiment 1 Thraustochytrium
[0021] 1. Medium
[0022] 1.1 Seed medium (per L)
[0023] Glucose 20g, yeast powder 2g, NaCl25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g, NaHCO 3 0.1g, vitamin B 1 0.01mg, vitamin B 12 0.001 mg.
[0024] 1.2 Fermentation medium (per L)
[0025] Tapioca starch 80g, yeast powder 15g, NaCl 25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g,NaHCO 3 0.1g, vitamin B 1 0.012mg, vitamin B 12 0.0012mg, biotin 0.05mg, initial pH 6.5.
[0026] 2. Training method
[0027] 2.1 Seed culture: Culture in a shaker flask, take a Thraustochytrium slant, add 7ml of sterile water, scrape off the spores with a flat spatula, inoculate them in the seed medium after shaking evenly, at 28°C, 170r / min Under activation for 48h.
[0028] 2.2 Fermentation culture: Under the protection of a fire ring, inoculate the seeds in a 500L fermenter with 300L ferm...
Embodiment 2
[0031] The fermentation process 2 of embodiment 2 Thraustochytrium
[0032] 1. Medium
[0033] 1.1 Seed medium (per L)
[0034] Glucose 15g, yeast powder 2g, NaCl10g, MgSO 4 ·7H 2 O4g, KCl0.5g, KH 2 PO 4 0.05g, CaCO 3 0.15g, (NH 4 ) 2 SO 4 0.15g, NaHCO 3 0.1g, Vitamin B 1 0.005mg, vitamin B 12 0.003mg.
[0035] 1.2 Fermentation medium (per L)
[0036] Tapioca starch 50g, yeast powder 10g, NaCl 20g, MgSO 4 ·7H 2 O4g, KCl, 0.5g, KH 2 PO 4 0.05g, CaCO 3 0.1g, (NH 4 ) 2 SO 4 0.1g,NaHCO 3 0.05g, vitamin B 1 0.02mg, vitamin B 12 0.002mg, biotin 0.1mg, initial pH 6.0.
[0037] 2. Training method
[0038] 2.1 Seed culture: Culture in a shaker flask, take a Thraustochytrium slant, add 7ml of sterile water, scrape off the spores with a flat spatula, inoculate them in the seed medium after shaking evenly, at 25°C, 180r / min Under activation for 60h.
[0039] 2.2 Fermentation culture: Under the protection of a fire ring, inoculate the seeds in a 500L fermenter with...
Embodiment 3
[0042] Fermentation process 3 of embodiment 3 Thraustochytrium
[0043] 1. Medium
[0044] 1.1 Seed medium (per L)
[0045] Glucose 20g, yeast powder 2g, NaCl25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g, NaHCO 3 0.1g, vitamin B 1 0.01mg, vitamin B 12 0.001mg.
[0046] 1.2 Fermentation medium (per L)
[0047] Tapioca starch 80g, yeast powder 15g, NaCl 25g, MgSO 4 ·7H 2 O5g, KCl1g, KH 2 PO 4 0.1g, CaCO 3 0.2g, (NH 4 ) 2 SO 4 0.2g,NaHCO 3 0.1g, vitamin B 1 0.012mg, vitamin B 12 0.0012mg, biotin 0.05mg, initial pH 6.5.
[0048] 2. Training method
[0049] 2.1 Seed culture: Culture in a shaker flask, take a Thraustochytrium slant, add 7ml of sterile water, scrape off the spores with a flat spatula, inoculate them in the seed medium after shaking evenly, at 28°C, 170r / min Under activation for 48h.
[0050] 2.2 Fermentation culture: Under the protection of a fire ring, inoculate the seeds in a 500L fermenter with 300L ferme...
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