Application of schizochytrium limacinum and preparation thereof in increasing of content of DHA in milk of ruminants

A technology for ruminants and Schizochytrium, applied in the biological field, can solve the problems of difficult processing technology, decreased milk fat, and reduced dietary feed intake

Active Publication Date: 2018-12-25
XIAMEN HUISON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rumen protection technologies for fatty acids on the market mainly include coating, hydrogenation, calcification, etc. Not only are the processing techniques difficult and the produ

Method used

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  • Application of schizochytrium limacinum and preparation thereof in increasing of content of DHA in milk of ruminants
  • Application of schizochytrium limacinum and preparation thereof in increasing of content of DHA in milk of ruminants
  • Application of schizochytrium limacinum and preparation thereof in increasing of content of DHA in milk of ruminants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the separation and identification of schizopot HS01

[0056] 1. Separation of schizopot HS01

[0057] 1. The inventor of the present application collected fissure pots from many mangrove forests in Yunxiao County, Zhangzhou City, Fujian Province, and mixed them to obtain a mixture; inoculated 0.5mL of the mixture into 5mL of screening liquid medium, and then cultured at 25°C and 200rpm / min 2d, obtain culture liquid.

[0058] 2. Spread the cultured bacteria solution obtained in step 1 evenly on the screening plate, and culture it at 25°C for 2 days to produce a single colony.

[0059] 3. After completing step 2, pick a single colony and inoculate it into 5 mL of fermentation medium, and then culture at 25°C and 200 rpm / min for 2 days to obtain a culture liquid.

[0060] 4. Take the cultured bacteria liquid obtained in step 3, centrifuge at 4°C and 2000 rpm for 5 minutes, and collect the bacteria.

[0061] 5. Take 1.0-2.0 g of bacteria to a stoppered meas...

Embodiment 2

[0075] Embodiment 2, the preparation of Schizochytrium powder

[0076] The steps of preparing Schizochytrium powder by using the Schizochytrium HS01 of Example 1 are as follows, the experiment is repeated three times, and the results are averaged:

[0077] 1. Preparation of culture medium

[0078] Shake flask culture medium 1 is made up of solute and solvent, and solvent is water, and solute and its concentration are 60g / L glucose, yeast extract 5g / L respectively; Shake flask culture medium 2 is made up of solute and solvent, and solvent is water, and solute and its concentration Concentrations were 150g / L glucose and 25g / L yeast extract.

[0079] Seed medium 1 is composed of solute and solvent, the solvent is water, the solute and its concentration are glucose 60g / L, yeast extract 8g / L, yeast powder 3g / L, Na 2 SO 4 5g / L, KCl 0.5g / L, MgSO 4 1.0g / L, K 2 SO 4 0.5g / L, KH 2 PO 4 1.0g / L, (NH 4 ) 2 SO 4 2.0g / L, CaCl 2 0.5g / L, CuSO 4 0.001g / L, ZnSO 40.001g / L, use ...

Embodiment 3

[0118] The Schizochytrium powder of embodiment 3, embodiment 2 can improve DHA content in milk

[0119] In this example, cows were fed the Schizochytrium powder of Example 2, and the DHA content in milk was detected to determine the effect of the Schizochytrium powder on the DHA content in milk. The experiment was repeated three times.

[0120] 1. Feeding method:

[0121] Select 60 healthy Holstein dairy cows with no significant difference in body weight and month age, and divide them into six groups randomly, 10 cows in each group, that is, free-range feeding experimental group 1, free-range feeding experimental group 2, free-range feeding blank control group, captive breeding experimental group 1. Captive breeding experimental group 2 and captive blank control group.

[0122] Firstly, the cows in each experimental group were reared in the pre-feeding period (the pre-feeding period was 15 days, and the feeding amount of Schizochytrium algae powder was gradually increased acc...

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Abstract

The invention discloses application of schizochytrium limacinum and preparation thereof in increasing of content of DHA in milk of ruminants. The preservation number of the schizochytrium limacinum atChina General Microbiological Culture Collection Center is CGMCC No. 13746. By schizochytrium limacinum powder prepared from the schizochytrium limacinum of the invention, the content of DHA in milkof the ruminants can be increased. The natural-source high-DHA-content milk is organic, safe and stable, is easy to absorb, and is safe and effective for people to take natural DHA, demands of consumers can be catered and met, and thus, the schizochytrium limacinum and the schizochytrium limacinum powder can be widely applied to the field of common food and the field of livestock breeding.

Description

technical field [0001] The invention relates to the application of Schizochytrium sp. and its preparation in increasing the DHA content in ruminant milk in the field of biotechnology. Background technique [0002] DHA (docosahexaenoic acid) belongs to the omega-3 series of polyunsaturated fatty acids (omega-3PUFAs), and is an important component of human cell membranes and nerve tissues. It plays an important role in promoting the growth and development of infants, preventing cardiovascular diseases, inhibiting and treating certain cancers, and ensuring the normal work of the nervous system. [0003] With the improvement of living standards and consumption levels and the deepening of the research on the physiological function of DHA, more and more attention has been paid to the DHA intake of different groups of people. According to the "Chinese DHA Consumption Survey" completed by the China Food Association, the daily direct DHA intake of the Chinese public from food is onl...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12P7/6427C12N1/125C12R2001/89
Inventor 陈礼毅钟惠昌陈水荣
Owner XIAMEN HUISON BIOTECH
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