Mutant strain of Schizochytrium limacinum

A technology of Schizochytrium and mutant strains, which is applied in the field of Schizochytrium mutant strains, can solve the problem of low DHA content in oils and fats, and achieve the effect of simple production process and short fermentation cycle

Inactive Publication Date: 2017-12-29
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the strain has a high DHA yield and has the potential for

Method used

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  • Mutant strain of Schizochytrium limacinum

Examples

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Embodiment 1

[0023] Example 1. Breeding method of Schizochytrium mutant strain

[0024] (1) Take CCTCC NO: M2012074 as the starting strain.

[0025] (2) The above-mentioned strains were cultured in the activation medium, the culture temperature was 28°C, the shaking speed of the shaker was 200r / min, and the culture was 48h to the logarithmic growth phase.

[0026] (3) Take 1ml of the activated seed culture solution of the above step (2) and inject it into a sterile petri dish and irradiate it with an ultraviolet lamp. The irradiation distance of the ultraviolet lamp is 30 cm, the irradiation time is 60 seconds, and the power of the ultraviolet lamp is 30 watts.

[0027] (4) The bacterial liquid after UV mutagenesis is air-dried aseptically to become plaque. Move the petri dish containing plaque aseptically into the high-energy particle beam injection machine, and pass the high-energy N with an energy of 20KeV + Ion beam implantation mutagenesis, N + Ion beam implantation dose is 100*10 17 ions / cm ...

Embodiment 2

[0036] Example 2 Production of docosahexaenoic acid using a mutant strain of Schizochytrium

[0037] (1) Seed activation culture: Take the Schizochytrium mutant strain of the present invention with the deposit number: CCTCC NO: M2012494-inoculate it into an activation medium and cultivate it at a culture temperature of 28°C and a shaking speed of 200 r / min , The culture time is 48h, the activation medium is: glucose 10g / L, sodium glutamate 25g / L, yeast extract 10g / L, sodium chloride 20g / L, magnesium sulfate 0.5g / L, natural pH.

[0038] (2) Seed expansion culture: Inoculate the activated seed culture solution of the above step (1) shaking culture into the expansion medium according to the 10% (volume ratio) inoculum for cultivation. The culture temperature is 28°C, and the culture time is 48 h. The bed shaking speed is 200 rpm, and the expanded medium in the shake flask is: glucose 40g / L, sodium glutamate 25g / L, yeast extract 10g / L, sodium chloride 10g / L, magnesium sulfate 5g / L, p...

Embodiment 3

[0042] Example 3 Production of docosahexaenoic acid using a mutant strain of Schizochytrium

[0043] (1) Seed activation culture: The Schizochytrium mutant strain of the deposit number of the present invention: CCTCC NO: M2012494 is inoculated into an activation medium and cultivated at a culture temperature of 28°C and a shaking speed of 150-250r / min, culture time 48h, the activation medium is: glucose 10g / L, sodium glutamate 25g / L, yeast extract 10g / L, sodium chloride 20g / L, magnesium sulfate 0.5g / L, natural pH.

[0044] (2) Seed expansion culture: inoculate the activated seed culture solution of the above step (1) shaking culture into an expansion medium at 10% (volume ratio) inoculum for cultivation. The volume of the seed tank used is 10L, and the seed tank is cultivated The basic volume is 60% (volume ratio), and the culture process technology is controlled as follows: culture temperature 28℃, stirring speed 150 revolutions / min, aeration volume 1 vvm (L / L.min), culture time...

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Abstract

The invention provides a mutant strain of Schizochytrium limacinum. The mutant strain of Schizochytrium limacinum is preserved in China Center for Type Culture Collection (CCTCC) on December 3rd, 2012, in Wuhan University, Wuhan, Hubei Province, China, with an accession number of CCTCC No. M2012494. In microbial oil produced by using a method which employs the mutant strain of Schizochytrium limacinum, the content of DHA is no less than 50 wt%. The oil contains a proper amount of beta-carotene, and since beta-carotene has antioxidation characteristics like removal of free radicals, so the stability of docosahexenoic acid oil can be enhanced.

Description

Technical field [0001] The invention relates to a mutant strain of Schizochytrium. Background technique [0002] Docosahexaenoic acid (DHA) is a long-chain polyunsaturated fatty acid with important physiological functions, which can promote brain and vision development and improve intelligence, so it is widely added to infant milk powder. In addition, DHA has the physiological functions of preventing and treating cardiovascular diseases, preventing Alzheimer's disease, and inhibiting cell carcinogenesis, so it is widely used in the food industry. [0003] DHA cannot be synthesized in large amounts in the human body, but it is indispensable and needs to be ingested from food. Traditional DHA is derived from deep-sea fish oil, but has the disadvantages of limited resources and high production costs, and its output is far from meeting market demand. At present, marine microalgae, including Schizochytrium, Thraustochytrium, Cryptodinium, etc., can synthesize DHA, and they have the ad...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12P7/6463C12P7/6472C12N1/145C12R2001/645C12N1/14C12P7/64
Inventor 汪志明李翔宇熊圆圆马凡提陆姝欢余超
Owner CABIO BIOTECH WUHAN CO LTD
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