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Acinetobacterbeijerinckii and application of acinetobacterbeijerinckii

A bacillus, plant growth promotion technology, applied in the direction of application, bacteria, animal repellent, etc., can solve the problems of no growth, low bioavailability, small biomass, etc., and achieve the effect of growth promotion

Inactive Publication Date: 2012-11-21
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also problems in the implementation of phytoremediation, such as low bioavailability of oil and saline-alkali soil, slow or no growth of plants, and small biomass, which all affect the effect of phytoremediation
The joint stress of oil and salinity makes restoration more difficult

Method used

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  • Acinetobacterbeijerinckii and application of acinetobacterbeijerinckii
  • Acinetobacterbeijerinckii and application of acinetobacterbeijerinckii
  • Acinetobacterbeijerinckii and application of acinetobacterbeijerinckii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Embodiment 1, isolation and identification of Acinetobacter beijerinckii LJL-12

[0037] 1. Obtaining of strain LJL-12

[0038] 1. Sampling

[0039] The soil was taken from the rhizosphere soil of barnyardgrass growing in the saline-alkali soil near the oil production plant in Daqing City, Heilongjiang Province. The rhizosphere soil was put into sterilized collection bottles and brought back to the laboratory, and stored in a 4°C refrigerator for later use.

[0040] 2. Screening and purification

[0041] (1) Take 1g of rhizosphere soil sample in 50mL of PAF medium, shake culture at 28°C. PAF medium contains peptone, casein hydrolyzate, MgSO 4 、K 2 HPO 4 and glycerin.

[0042] (2) Take 1mL of the shaken PAF culture solution from (1), put it in 50mL of PAF medium, and culture it with shaking at 28°C.

[0043] (3) Take 1 mL of the PAF culture solution obtained in (2), place it in 50 mL of the DF salt culture solution, and culture it with shaking at 28°C. DF salt med...

Embodiment 2

[0061] Embodiment 2, the ability of Acinetobacter beijerinckii LJL-12 to produce ACC deaminase

[0062] Experimental mechanism: 1-aminocyclopropane-1-carboxylic acid is degraded under the action of ACC deaminase to generate α-butyruvate (α-KA) and ammonia gas.

[0063] The experimental steps are as follows:

[0064] 1. Cultivate Acinetobacter beijerinckii LJL-12 overnight in TSB culture medium, collect the bacteria by centrifugation at 4°C and wash three times with DF culture medium.

[0065] 2. Resuspend the bacteria obtained in step 1 in ADF culture medium, shake and culture at room temperature (21±1°C) for 2 days, collect the bacteria by centrifugation at 4°C, and wash with 0.1mol / L Tris-HCl buffer (pH7.6) three times.

[0066] 3. Resuspend the bacteria obtained in step 2 in 600 μL 0.1mol / L Tris-HCl buffer (pH8.0), add 30 μL toluene and shake rapidly for 30 seconds (using a vortex shaker) to break the cells. The system is the cell extract.

[0067] 4. Group processing ...

Embodiment 3

[0074] Example 3, Ability of Acinetobacter beijerinckii LJL-12 to Produce Growth Hormone (Indoleacetic Acid, IAA)

[0075] 1. Incubate Acinetobacter beijerinckii LJL-12 in DF culture medium at 28°C and 180r min -1 Shake culture for 2 days, and then micro-transfer into DF culture medium supplemented with different concentrations of tryptophan (L-Trp) (the concentration of L-Trp is 0, 50, 100, 200 or 500 μg·mL -1 ) at 28°C, 180r·min -1 Shake culture for 2 days, take a sample to test the OD of the bacterial solution 600 (OD 600nm Numerical value), and the rest of the culture medium was centrifuged at 8000g at room temperature to obtain the supernatant.

[0076] 2. Take 500 μL of the supernatant from step 1, add 2 mL of Salkowski reagent (150 mL of H 2 SO 4 , 250mLddH 2 O and 7.5mL 0.5mol / L FeCl 3 aqueous solution, the rest is water), incubated in the dark at room temperature for 20min and measured the absorbance at 535nm (OD 535nm value).

[0077] The DF culture medium w...

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Abstract

The invention discloses a strain of acinetobacterbeijerinckii and application of the acinetobacterbeijerinckii. The acinetobacterbeijerinckii LJL-12 provided by the invention has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.6291. The acinetobacterbeijerinckii LJL-12 has the following application values that the acinetobacterbeijerinckii LJL-12 can grow in the environment adopting ACC (aminocyclopropane carboxylic acid) as the unique nitrogen source, and meanwhile, the ACC is decomposed; IAA (indole acetic acid) can be synthesized, and in addition, the IAA synthesis quantity is increased along with the concentration increase of L-Trp; siderophores can be synthesized; and the promotion effect is realized on the oat growth in petroleum polluted saline-alkali soil. The acinetobacterbeijerinckii LJL-12 is hopeful to realize the important effect in the petroleum polluted saline-alkali soil plant restoration.

Description

technical field [0001] The invention relates to a strain of Acinetobacter beijerinckii and its application. Background technique [0002] Petroleum has become an indispensable energy source for human life and has made great contributions to human civilization and social progress. However, due to various reasons, oil has entered the environment, causing environmental pollution and endangering human existence. Soil salinization is currently one of the global soil degradation problems. Soil salinity is an important environmental factor affecting plant growth and yield. Salt-alkali stress will affect almost all important life processes of plants, causing serious damage to agricultural production. Great loss. [0003] Phytoremediation of polluted soil has become the focus of attention all over the world because of its low cost, good effect and no secondary pollution. However, there are also problems in the implementation of phytoremediation, such as low bioavailability of oil ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/88C12P17/10C12P1/04A01N63/02A01P21/00C12R1/01
Inventor 郭长虹刘佳莉方芳蔡洪生谢宝明
Owner HARBIN NORMAL UNIVERSITY
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