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Composite germ pulp, and production method and application thereof

A production method and technology of compound bacteria, which are applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of polluting the environment, high costs, hindering the development of the amino acid production industry, etc., and improve the survival rate. , Improve quality and inhibit the growth of pathogenic bacteria in water

Active Publication Date: 2012-02-15
宜兴市天石饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Amino acid waste slurry contains a large amount of organic pollutants such as carbohydrates, organic heteroacids, and proteins. Direct discharge will pollute the environment, and high costs will be required to treat and discharge after reaching the standard, which has become an important issue hindering the development of the amino acid production industry.

Method used

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  • Composite germ pulp, and production method and application thereof
  • Composite germ pulp, and production method and application thereof
  • Composite germ pulp, and production method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0049] The amino acid waste slurry obtained from Jiangsu Hanguang Bioengineering Co., Ltd. was used as the raw material. The waste slurry mainly contains carbohydrates, organic acids, proteins, amino acids, B vitamins, nitrogen, phosphorus, potassium and other nutrients, with a total solid content of 6.7%.

[0050] Production steps:

[0051] (1) Paenibacillus polymyxa liquid spawn preparation:

[0052] The preparation method of the liquid medium used (taking 1000ml as an example): Weigh 100g of potatoes, cut into 1cm cubes, boil in 1000ml of pure water for 30min, filter, take the filtrate, add purified water to 1000ml, add glucose 10g, hydrogen phosphate Dipotassium 2.5g, potassium dihydrogen phosphate 1.5g, adjust the pH to 6.5-7.0 with acetic acid or sodium hydroxide, and sterilize at 121°C for 30 minutes; when the temperature is lowered to 31°C, place the inoculated strains on a shaking table and adjust the temperature to 30°C for shaking culture; The shaker rotates at 11...

preparation Embodiment 2

[0059] Production steps:

[0060] (1) Paenibacillus polymyxa liquid spawn preparation:

[0061] The preparation method of the liquid medium used (taking 1000ml as an example): Weigh 100g of potatoes, cut them into 1cm cubes, put them in 1000ml of distilled water and boil for 30min, filter, take the filtrate, add water to make up to 1000ml, add 10g of glucose, dipotassium hydrogen phosphate 2.5g, potassium dihydrogen phosphate 1.5g, adjust the pH to 6.5-7.0 with sodium hydroxide, sterilize at 121°C for 30min; min, cultured for 24 hours, and made into liquid strains, the content of Paenibacillus polymyxa in the bacterial liquid was ≥1×10 10 CFU / ml;

[0062] (2) Preparation of liquid strain of Rhodobacter sphaeroides:

[0063] The preparation method of the liquid medium used (taking 1000ml as an example): in 1000ml of distilled water, add 1g of ammonium sulfate, 200mg of magnesium sulfate, 100mg of ferrous sulfate, 5mg of manganese sulfate, 60mg of zinc sulfate, 4mg of copper ...

preparation Embodiment 3

[0068] Composite microbial granules used for purification of aquaculture water are produced using composite bacterial slurry as raw material.

[0069] formula:

[0070] Composite bacteria slurry (prepared in Example 1) 1kg;

[0071] Zeolite powder (80 mesh) 7kg.

[0072] Preparation steps:

[0073] (1) Stir and mix the compound bacterium slurry of above-mentioned formula quantity with zeolite powder, make the loose mixture that zeolite powder and bacterium slurry mix;

[0074] (2) Send the above-mentioned powdery mixture into a granulator to make Φ2-Φ5mm granules, and dry at 50°C until the water content is less than 20% to obtain the finished product of composite microbial granules.

[0075] The obtained granules contain Bacillus polymyxa ≥ 3 × 10 8 CFU / g, Rhodobacter sphaeroids ≥2×10 8 CFU / g.

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Abstract

The invention discloses composite germ pulp, and a production method and an application thereof, which belong to the field of microorganism application. The composite germ pulp is prepared in the following way that amino acid waste pulp is used as a fermentation substrate, paenibacillus polymyxa with the inoculation quantity being 6 percent and rhodobacter sphaeroides with the inoculation quantity being 4 percent are inoculated, the fermentation is carried out for 24h at the pH value being 6.5 to 7.0 and the temperature being 30 DEG C and 34 DEG C under the oxybiontic condition, and the composite germ pulp is obtained. The paenibacillus polymyxa content in the composite germ pulp is higher than or equal to 5*10<9> CFU / ml, the rhodobacter sphaeroides content is higher than or equal to 3*10<9> CFU / ml, and the composite germ pulp can be applied to culture water body purification or organic fertilizer preparation. Compared with the prior art, the composite germ pulp uses the amino acid waste pulp as the fermentation substrate, has the characteristics of low production cost, energy saving, environment protection and the like and can be widely applied to the culture water body purification and organic fertilizer production.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to a composite bacteria slurry, its production method and its application. Background technique [0002] At present, there are nearly 100 enterprises engaged in the production of amino acids in my country, and the annual output has exceeded 3 million tons. The main raw materials for amino acid production are corn and rice; the annual consumption is 4.326 million tons, and every ton of amino acid produced produces 7-10 tons of waste slurry. Amino acid waste slurry contains a large amount of organic pollutants such as carbohydrates, organic heteroacids, and proteins. Direct discharge will pollute the environment. However, it will cost a lot to discharge after treatment, which has become an important problem hindering the development of the amino acid production industry. How to fully recycle the waste amino acid slurry has become a technical problem that those skilled in the ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C05F11/08C12R1/01
Inventor 王栋白志辉吴美君李磊张艳凌德霞关晓欢陆震蒋杰
Owner 宜兴市天石饲料有限公司
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