Rhodobacter sphaeroides strain and preparation method and application thereof
A technology of Rhodobacter sphaeroides and strains, which is applied in the field of production of Rhodobacter sphaeroides coenzyme Q10, can solve the problem of low yield of products with genetic characteristics of strains, and achieve the effects of increasing production and reducing costs
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[0043] 1. Strains and experimental materials
[0044] All the strains and materials in this example were purchased and sold in the market. For example, Rhodobacter sphaeroides 2.4.1 was purchased from ATCC (strain number ATCC-17023), and Acinetobacter calcoaceticus was purchased from ATCC (strain number ATCC-31926). Plasmid and genome extraction kit were purchased from TIANGEN company. The PCR product recovery kit was purchased from Omega, USA. T 4 Ligase was purchased from NEB, USA. The chemical reagents were purchased from Sinopharm Chemical Reagent Co., Ltd., all of which were of analytical grade.
[0045] 2. Construction of pBBR1MCS-2-pcaK vector:
[0046] The p-hydroxybenzoic acid transporter (pcaK) gene was cloned from the genome of Acinetobacter calcium acetate:
[0047] Acinetobacter calcium acetate was inoculated into a 50mL Erlenmeyer flask containing LB medium and cultured overnight for 8 hours. The bacteria were collected by centrifugation. The genome of Acinetobacter...
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